Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,032,504	C→T	V30I (GTA→ATA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,032,504	0	C	T	100.0%	66.5 / NA	27	V30I (GTA→ATA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/16);  total (11/16)

TTTTAAAACTTTTCGCATTTTTTATTCTTGTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATACGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATAGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGT  >  1/1032321‑1032726                                                                                                                                                                                        |                                                                                                                                                                                                                               ttttAAAACTTTTCGCATTTTTTATTCTTGTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATg                                                                                                                                                                               <  1:139795/233‑1 (MQ=255)                   tttttATTCTTGTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAg                                                                                                                                                              <  1:164242/232‑1 (MQ=255)                             tGTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGt                                                                                                                                 >  1:504807/1‑251 (MQ=255)                              gTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAg                                                                                                                                                              <  1:846559/221‑1 (MQ=255)                              gTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAg                                                                                                                                                              <  1:463484/221‑1 (MQ=255)                              gTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGtt                                                                                                                                >  1:714315/1‑251 (MQ=255)                                cTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTa                                                                                                                               >  1:512036/1‑250 (MQ=255)                                           tAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAAc                                                                                                                   <  1:614234/251‑1 (MQ=255)                                                                    cgcCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                          <  1:641532/251‑1 (MQ=255)                                                                      cccTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATg                                                                                                                                                                               <  1:704003/164‑1 (MQ=255)                                                                      cccTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTc                                                                                                                 >  1:521142/1‑226 (MQ=255)                                                                           gTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGCTCGGTGTTTTACATCCATAAAATGAAAATAATATGAAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGTGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGta                                                                                   >  1:832206/1‑251 (MQ=255)                                                                                 aTTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                             <  1:809627/251‑1 (MQ=255)                                                                                 aTTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                             <  1:730142/251‑1 (MQ=255)                                                                                    ggTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAg                                                                                     >  1:312579/1‑240 (MQ=255)                                                                                    ggTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                             <  1:768109/248‑1 (MQ=255)                                                                                          gTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAgg                                                                    >  1:664625/1‑251 (MQ=255)                                                                                                              aTTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACt                                                                                                                                                                                    >  1:87784/1‑119 (MQ=255)                                                                                                              aTTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                          >  1:139164/1‑209 (MQ=255)                                                                                                                     aTTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAAc                                                                                       <  1:900721/205‑1 (MQ=255)                                                                                                                          cccTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCt                                                                                                   <  1:715202/188‑1 (MQ=255)                                                                                                                               gTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGAc                                                                                                           <  1:222845/175‑1 (MQ=255)                                                                                                                                                    tGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATgg                                          <  1:764738/219‑1 (MQ=255)                                                                                                                                                            gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAAc                                                                                                                   <  1:393433/138‑1 (MQ=255)                                                                                                                                                            gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACt                                                                                                                  <  1:544446/139‑1 (MQ=255)                                                                                                                                                            gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                          >  1:707265/1‑163 (MQ=255)                                                                                                                                                            gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGgtgt  >  1:147877/1‑251 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                                               TTTTAAAACTTTTCGCATTTTTTATTCTTGTCTATTCTTCAATAAAAAAGGACGCCGTTTTTTCTTGCGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATACGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATAGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGT  >  1/1032321‑1032726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑