Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,025,097	T→G	T439P (ACG→CCG)	unknown ←	Cardiolipin synthase, bacterial type ClsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,025,097	0	T	G	100.0%	195.0 / NA	71	T439P (ACG→CCG)	unknown	Cardiolipin synthase, bacterial type ClsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/34);  total (37/34)

TACGACCGTGGCGGATTAAATAAAAAGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGTATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAATCGTTTTCTTTCGGCAGAATAATGGACACCTCCACGCCA  >  1/1024850‑1025335                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               tACGACCGTGGCGGATTAAATAAAAAGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATc                                                                                                                                                                                                                                             <  1:721952/251‑1 (MQ=255)            cGGATTAAATAAAAAGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTccaccacc                                                                                                                                                                                                                                   <  1:162286/250‑1 (MQ=255)               aTTAAATAAAAAGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAg                                                                                                                                                                                                                               <  1:707344/251‑1 (MQ=255)                        aaaGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTc                                                                                                                                                                                                                        >  1:743794/1‑249 (MQ=255)                                       ttCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGc                                                                                                                                                                                                       <  1:791680/251‑1 (MQ=255)                                           ttattaTCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGcctg                                                                                                                                                                                                    >  1:422567/1‑247 (MQ=255)                                           ttattaTCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTgcg                                                                                                                                                                                                   >  1:553124/1‑251 (MQ=255)                                           ttattaTCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTgcg                                                                                                                                                                                                   >  1:185702/1‑251 (MQ=255)                                           ttattaTCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTgcg                                                                                                                                                                                                   >  1:834312/1‑251 (MQ=255)                                             attatCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTc                                                                                                                                                                                                                             >  1:49074/1‑223 (MQ=255)                                                atCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCatat                                                                                                                                                                                              >  1:307421/1‑251 (MQ=255)                                                  ccTTGAATAAATGGTAACGAGAAAAAAACCCCGAGAAAATCAACCGCCCTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATcc                                                                                                                                                                                            <  1:778915/251‑1 (MQ=255)                                                      gATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTccacc                                                                                                                                                                                                                                      >  1:818801/1‑205 (MQ=255)                                                      gATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATg                                                                                                                                                                                         >  1:459640/1‑250 (MQ=255)                                                       aTTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGt                                                                                                                                                                                        <  1:562400/250‑1 (MQ=255)                                                       aTTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGt                                                                                                                                                                                        <  1:819555/250‑1 (MQ=255)                                                       aTTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAACTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGt                                                                                                                                                                                        <  1:449920/250‑1 (MQ=255)                                                            aTGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCaaa                                                                                                                                                                                                                     >  1:862495/1‑216 (MQ=255)                                                            aTGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACgg                                                                                                                                                                                  >  1:315160/1‑251 (MQ=255)                                                            aTGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCAATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACgg                                                                                                                                                                                  >  1:410978/1‑251 (MQ=255)                                                                     agaTAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCaaa                                                                                                                                                                                                                     <  1:718209/207‑1 (MQ=255)                                                                     agaTAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGAAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGAcc                                                                                                                                                                          <  1:63016/250‑1 (MQ=255)                                                                      gaTAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCaa                                                                                                                                                                        >  1:38673/1‑251 (MQ=255)                                                                        tAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTc                                                                                                                                                                                                                             >  1:308319/1‑196 (MQ=255)                                                                                    gaAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTAt                                                                                                                                                          >  1:718782/1‑251 (MQ=255)                                                                                    gaAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTAt                                                                                                                                                          >  1:516813/1‑251 (MQ=255)                                                                                     aaaaTCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTa                                                                                                                                                           <  1:457063/249‑1 (MQ=255)                                                                                                 aCTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACt                                                                                                                                             <  1:208020/251‑1 (MQ=255)                                                                                                    ttttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGGTCTTCCACCACCATCGTCACTTCAAAATTCAATAAAAATCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACtttt                                                                                                                                          >  1:580854/1‑251 (MQ=255)                                                                                                    ttttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATcc                                                                                                                                                                                            >  1:96661/1‑201 (MQ=255)                                                                                                    ttttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATg                                                                                                                                                                                         >  1:213637/1‑204 (MQ=255)                                                                                                    ttttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACtttt                                                                                                                                          >  1:499328/1‑251 (MQ=255)                                                                                                      ttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTTATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAg                                                                                                                                        <  1:521557/251‑1 (MQ=255)                                                                                                      ttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAg                                                                                                                                        <  1:175461/251‑1 (MQ=255)                                                                                                      ttACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAg                                                                                                                                        <  1:634334/251‑1 (MQ=255)                                                                                                       tactacAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAgt                                                                                                                                       >  1:734350/1‑251 (MQ=255)                                                                                                       tactacAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAgt                                                                                                                                       >  1:465322/1‑251 (MQ=255)                                                                                                           acaacaGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGc                                                                                                                                   <  1:788343/251‑1 (MQ=255)                                                                                                                  cGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAAcc                                                                                                                            <  1:209696/251‑1 (MQ=255)                                                                                                                        gAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCaa                                                                                                                                                       <  1:162916/218‑1 (MQ=255)                                                                                                                                                  tGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAAc                                                                                                                             <  1:731539/218‑1 (MQ=255)                                                                                                                                                      ttGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTc                                                                                                                                                                                                                             >  1:172441/1‑118 (MQ=255)                                                                                                                                                      ttGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATg                                                                                                                                                     >  1:66474/1‑190 (MQ=255)                                                                                                                                                        gATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTccaccac                                                                                                                                                                                                                                    >  1:379409/1‑109 (MQ=255)                                                                                                                                                                   cGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTccaccacca                                                                                                                                                                                                                                  <  1:520206/100‑1 (MQ=255)                                                                                                                                                                   cGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCaaa                                                                           >  1:621098/1‑251 (MQ=255)                                                                                                                                                                     aTTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTa                                                                                                                                                           >  1:543458/1‑169 (MQ=255)                                                                                                                                                                             aCTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGa                                                                 >  1:215512/1‑251 (MQ=255)                                                                                                                                                                              cTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGAc                                                                <  1:837601/251‑1 (MQ=255)                                                                                                                                                                              cTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGAc                                                                <  1:116025/251‑1 (MQ=255)                                                                                                                                                                                 ggCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTg                                                             >  1:406409/1‑251 (MQ=255)                                                                                                                                                                                     tGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAg                                                                                       <  1:90322/221‑1 (MQ=255)                                                                                                                                                                                      gATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATg                                                                                                                                                     <  1:420592/158‑1 (MQ=255)                                                                                                                                                                                      gATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAatcat                                                                               <  1:546328/228‑1 (MQ=255)                                                                                                                                                                                      gATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGAc                                                                <  1:786456/243‑1 (MQ=255)                                                                                                                                                                                      gATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAg                                                        >  1:149080/1‑251 (MQ=255)                                                                                                                                                                                               aTTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGc                                                                                                                                                                               <  1:433957/123‑1 (MQ=255)                                                                                                                                                                                               aTTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAg                                                                                                                                        <  1:773079/162‑1 (MQ=255)                                                                                                                                                                                               aTTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAatc                                                                                 >  1:463168/1‑217 (MQ=255)                                                                                                                                                                                               aTTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACcat                                                >  1:724918/1‑250 (MQ=255)                                                                                                                                                                                                ttCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACcatca                                              <  1:731958/251‑1 (MQ=255)                                                                                                                                                                                                       ttgttgATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAAt                                        >  1:231175/1‑250 (MQ=255)                                                                                                                                                                                                         gttgATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAAc                                                                                                                                                                                    <  1:858195/108‑1 (MQ=255)                                                                                                                                                                                                         gttgATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAg                                                                                                                                        >  1:714274/1‑152 (MQ=255)                                                                                                                                                                                                            gATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTa                                                                                                                                                                                      <  1:218792/103‑1 (MQ=255)                                                                                                                                                                                                            gATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAATCGtttt                                  >  1:573601/1‑251 (MQ=255)                                                                                                                                                                                                                    cTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTc                                                                                                                                                                                                                             <  1:715410/56‑1 (MQ=255)                                                                                                                                                                                                                    cTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAATCGTTTTCTTTCGGc                          <  1:595480/251‑1 (MQ=255)                                                                                                                                                                                                                      tCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACcat                                                <  1:565489/227‑1 (MQ=255)                                                                                                                                                                                                                                             ggCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGc                                                                                                                                                                               >  1:410107/1‑77 (MQ=255)                                                                                                                                                                                                                                             ggCGAACGCCGGATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAATCGTTTTCTTTCGGCAGAATAATGGACACCTccacgcca  >  1:247577/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               TACGACCGTGGCGGATTAAATAAAAAGTCAGATTTTTGTTCATTATTATCCTTGATTAAATGTTATCGAGATAAAAACACCGAGAAAATCAACCGCACTTTTTACTACAACAGCGGGCTGAAGAGGAAAAACAATTTTTCGATAATGCGTTGATAAACGGAACGATTTGCCCACTTGGCATGATCAGGTAATTCTGAATTGTTGATGTAACCTTCGTGGAGCAAGGAAATTTCATTGGCGAACGCCGTATCTTCCACCACCATAGTCACTTCAAAATTCAATAAAAAGCTGCGCATATCCATGTTAACGGTGCCGACCAAAGCGAGCTTGTTATCAATGAGCATACTTTTAGTGTGCAATAACCCTTTCTTGAAATTATAAATTTTTACGCCGGCGGCAAGTAAATCATCAAAAAAGGTACGACTTGCCCAGCGTACCATCATGGAATCGTTTTCTTTCGGCAGAATAATGGACACCTCCACGCCA  >  1/1024850‑1025335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑