Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,005,262	T→C	V14A (GTA→GCA)	unknown →	Outer membrane protein A precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,005,262	0	T	C	100.0%	126.5 / NA	46	V14A (GTA→GCA)	unknown	Outer membrane protein A precursor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

GATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAATTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGTAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGTTTATTC  >  1/1005026‑1005488                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   gATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCtt                                                                                                                                                                                                                      >  1:872490/1‑251 (MQ=255)                           cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTa                                                                                                                                                                                                           <  1:866680/236‑1 (MQ=255)                           cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTaaa                                                                                                                                                                                            >  1:780165/1‑251 (MQ=255)                           cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTaaa                                                                                                                                                                                            >  1:691544/1‑251 (MQ=255)                            tATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAaag                                                                                                                                                                                           <  1:114305/251‑1 (MQ=255)                                                                   aCTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCt                                                                                                                                                     >  1:310245/1‑250 (MQ=255)                                                                    cTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACt                                                                                                                                                                                                             <  1:94957/193‑1 (MQ=255)                                                                    cTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCa                                                                                                                                                   >  1:677299/1‑251 (MQ=255)                                                                       tAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAAt                                                                                                                                                <  1:43991/251‑1 (MQ=255)                                                                       tAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAAt                                                                                                                                                <  1:718607/251‑1 (MQ=255)                                                                             gTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGaaaa                                                                                                                                          >  1:436531/1‑251 (MQ=255)                                                                             gTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGaaaa                                                                                                                                          >  1:393364/1‑251 (MQ=255)                                                                                           aTACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTaagtaa                                                                                                                                                                                                      >  1:642926/1‑177 (MQ=255)                                                                                                  gCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACgg                                                                                                                     >  1:692296/1‑251 (MQ=255)                                                                                                       aaTTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGc                                                                                                                 >  1:394576/1‑250 (MQ=255)                                                                                                                      aTTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAAt                                                                                                                             >  1:201781/1‑223 (MQ=255)                                                                                                                      aTTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGc                                                                                                 <  1:183859/251‑1 (MQ=255)                                                                                                                                           ttattaTATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTa                                                                                                                                                           >  1:346470/1‑172 (MQ=255)                                                                                                                                             attatATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATg                                                                          <  1:62795/251‑1 (MQ=255)                                                                                                                                                atatCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATc                                                                                                                            <  1:895767/198‑1 (MQ=255)                                                                                                                                                atatCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACgg                                                                                                                     <  1:305813/205‑1 (MQ=255)                                                                                                                                                  atCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGa                                                                     >  1:90637/1‑251 (MQ=255)                                                                                                                                                    cTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGaaa                                                                   >  1:142186/1‑251 (MQ=255)                                                                                                                                                            ccTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCt                                                                                                                                                                                                                       <  1:551653/95‑1 (MQ=255)                                                                                                                                                            ccTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATg                                                           >  1:87824/1‑251 (MQ=255)                                                                                                                                                                 ggATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTa                                                                            >  1:725982/1‑229 (MQ=255)                                                                                                                                                                 ggATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATaa                                                      >  1:429088/1‑251 (MQ=255)                                                                                                                                                                    ttGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGAc                                                                                      >  1:865884/1‑216 (MQ=255)                                                                                                                                                                      gTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCg                                                                                                                                                                            >  1:57864/1‑128 (MQ=255)                                                                                                                                                                      gTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACAttt                                                                                                                                >  1:432393/1‑172 (MQ=255)                                                                                                                                                                      gTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGtt                                                                                                       <  1:867721/197‑1 (MQ=255)                                                                                                                                                                      gTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGa                                                   <  1:480722/249‑1 (MQ=255)                                                                                                                                                                      gTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGAt                                                  >  1:488282/1‑250 (MQ=255)                                                                                                                                                                       ttCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATc                                                 <  1:243163/250‑1 (MQ=255)                                                                                                                                                                       ttCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATc                                                 <  1:621455/250‑1 (MQ=255)                                                                                                                                                                                          aGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAAc                                      <  1:679111/242‑1 (MQ=255)                                                                                                                                                                                          aGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTg                               <  1:254543/249‑1 (MQ=255)                                                                                                                                                                                                         aaTTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGa                                                   <  1:673852/214‑1 (MQ=255)                                                                                                                                                                                                             aTCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAg          >  1:84492/1‑251 (MQ=255)                                                                                                                                                                                                               cacaTTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGt        <  1:128674/251‑1 (MQ=255)                                                                                                                                                                                                                 caTTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGttt      >  1:795906/1‑251 (MQ=255)                                                                                                                                                                                                                    ttCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCt                                                                                                                                                                                                                       <  1:393732/39‑1 (MQ=255)                                                                                                                                                                                                                     tCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGTTTATTc  <  1:82029/251‑1 (MQ=255)                                                                                                                                                                                                                     tCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGTTTATTc  <  1:184202/251‑1 (MQ=255)                                                                                                                                                                                                                     tCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGTTTATTc  <  1:114256/251‑1 (MQ=255)                                                                                                                                                                                                                         attatCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAg                                                                                                                                                             >  1:15700/1‑92 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   GATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAATTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGTAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGCCGAACTGGGACAATGTTCGTATGATTGAAAAAGGCATGAATAAAGATCAGCTTTATAACTTGATTGGTCGTCCGCACTTCGCTGAAGGTTTATTC  >  1/1005026‑1005488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑