Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,003,979	G→T	W2C (TGG→TGT)	unknown →	Possible protease sohB (EC 3.4.21.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,003,979	0	G	T	100.0%	107.1 / NA	39	W2C (TGG→TGT)	unknown	Possible protease sohB (EC 3.4.21.‑)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/16);  total (23/16)

GTGAACTGTCTGCCAGAATACTGGATTTTAATACAAGAACGTTGTTCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGGTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTAAAAGCGGCGGAAAAAGCCGAGAAAAAAGCGGAAAAAG  >  1/1003733‑1004220                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gtgAACTGTCTGCCAGAATACTGGATTTTAATACAAGAACGTTGTTCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCgg                                                                                                                                                                                                                                               >  1:51541/1‑251 (MQ=255)                gAATACTGGATTTTAATACAAGAACGTTGTTCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGtt                                                                                                                                                                                                                                >  1:245892/1‑251 (MQ=255)                    aCTGGATTTTAATACAAGAACGTTGTTCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAAGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATgg                                                                                                                                                                                                                            >  1:430975/1‑251 (MQ=255)                                            gttCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCa                                                                                                                                                                                                     >  1:170477/1‑250 (MQ=255)                                                       tccttcctAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTtg                                                                                                                                                                                         <  1:774157/251‑1 (MQ=255)                                                           tcctAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGAt                                                                                                                                                                                     >  1:38117/1‑251 (MQ=255)                                                                      tgaGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAACGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGCGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTgg                                                                                                                                                                          >  1:79627/1‑251 (MQ=255)                                                                                      tCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATCTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGc                                                                                                                                                          <  1:442166/251‑1 (MQ=255)                                                                                       cATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCa                                                                                                                                                         >  1:512609/1‑251 (MQ=255)                                                                                                                            ttttGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTgg                                                                                                                    <  1:361033/251‑1 (MQ=255)                                                                                                                                     gCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCg                                                                                                           >  1:59523/1‑251 (MQ=255)                                                                                                                                        cTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACtt                                                                                                                                                                                          <  1:826543/169‑1 (MQ=255)                                                                                                                                        cTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCAc                                                                                                                              >  1:124402/1‑229 (MQ=255)                                                                                                                                        cTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTgg                                                                                                                    <  1:194651/239‑1 (MQ=255)                                                                                                                                          aTTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTg                                                                                                                  >  1:786195/1‑239 (MQ=255)                                                                                                                                                    ggTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAAt                                                                                            >  1:704172/1‑251 (MQ=255)                                                                                                                                                                                      gCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATgg                                                                               <  1:805478/230‑1 (MQ=255)                                                                                                                                                                                             ggAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTg                                                                                                                  <  1:828248/188‑1 (MQ=255)                                                                                                                                                                                                  ggCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGa                                              >  1:459724/1‑251 (MQ=255)                                                                                                                                                                                                       ctcCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAAt                                         >  1:75669/1‑251 (MQ=255)                                                                                                                                                                                                       ctcCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAAt                                         >  1:35171/1‑251 (MQ=255)                                                                                                                                                                                                         ccTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTgg                                                                                                                    <  1:839679/174‑1 (MQ=255)                                                                                                                                                                                                         ccTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGc                                                                  <  1:462107/224‑1 (MQ=255)                                                                                                                                                                                                         ccTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTa                                       >  1:585945/1‑251 (MQ=255)                                                                                                                                                                                                         ccTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTa                                       >  1:609137/1‑251 (MQ=255)                                                                                                                                                                                                              aaTCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGc                                                                                                                                                          >  1:26442/1‑131 (MQ=255)                                                                                                                                                                                                              aaTCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGc                                                                                                                                                          <  1:124401/131‑1 (MQ=255)                                                                                                                                                                                                              aaTCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAAt                                                                       >  1:345300/1‑214 (MQ=255)                                                                                                                                                                                                              aaTCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAATCGATTTTCATCTCACCGAAGACGAATTAAAAgc                                  <  1:428263/251‑1 (MQ=255)                                                                                                                                                                                                               aTCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTAcc                                                                                                            >  1:348186/1‑176 (MQ=255)                                                                                                                                                                                                                   ggTCTCTTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTg                                                                                                                                                           >  1:9552/1‑125 (MQ=255)                                                                                                                                                                                                                      ctctTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCAtc                                                        >  1:375772/1‑221 (MQ=255)                                                                                                                                                                                                                        ctTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTgg                                                                                                                    <  1:287623/159‑1 (MQ=255)                                                                                                                                                                                                                        ctTGTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCAtctc                                                      <  1:662098/221‑1 (MQ=255)                                                                                                                                                                                                                           gTTAATAATATGAAGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCAtc                                                        <  1:70862/216‑1 (MQ=255)                                                                                                                                                                                                                                        aGGAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTAAAAGCGGCGGAAAAAGCCGAGAAAAAAGCgg        >  1:2124/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggAAAAATTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATcc                                                                                                   <  1:302335/159‑1 (MQ=255)                                                                                                                                                                                                                                            aaaaTTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTtg                                                                                                                                                                                         <  1:844103/70‑1 (MQ=255)                                                                                                                                                                                                                                              aaTTATGTGTTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTAAAAGCGGCGGAAAAAGCCGAGAAAAAAGCGGAAAAAg  >  1:146969/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTGAACTGTCTGCCAGAATACTGGATTTTAATACAAGAACGTTGTTCATTTTGTTCCTTCCTAAATGAATGAGTGAAAAGTGCGGTCATTTTAACGAATGTTTTTTTTAAATAAAGGCTTTTCTTTTGAAAAGCACTATTAACGATGGGTTAATAATCCGATTTATTTGAGAGATAAAGTAGCTTTAAGGAAAGGCTTCTCCTATAATCTGGTCTCTTGTTAATAATATGAAGGAAAAATTATGTGGTCGGAAATTTTTATTGGTTATGGTGTATTTCTTTTGGAAGTTATCACGCTATTACTTGTGATCGCCGCCGTGGTCGCGATGATTATTGCAATTAAACAGAAAAAAGCGCATTTGCACGGTGAATTGGTGATTACCGATTTATCCAAGGAATTTGAGGAAAATGGTAAAAAATTAAGCGATTTTCATCTCACCGAAGACGAATTAAAAGCGGCGGAAAAAGCCGAGAAAAAAGCGGAAAAAG  >  1/1003733‑1004220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑