Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	997,446	C→T	G449S (GGC→AGC)	unknown ←	Penicillin‑binding protein activator LpoA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	997,446	0	C	T	100.0%	161.5 / NA	59	G449S (GGC→AGC)	unknown	Penicillin‑binding protein activator LpoA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/27);  total (32/27)

TCATTGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCCTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGAATGCCGTCACGCCACATTTTGTTGGCGGCGGATTCCGC  >  1/997202‑997689                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    tCATTGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGTGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCaa                                                                                                                                                                                                                                               >  1:528939/1‑251 (MQ=255) tCATTGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCaa                                                                                                                                                                                                                                               >  1:604843/1‑251 (MQ=255)  cATTGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAg                                                                                                                                                                                                                                              >  1:753053/1‑251 (MQ=255)    ttGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTc                                                                                                                                                                                                                                            <  1:484906/251‑1 (MQ=255)           ggTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAAt                                                                                                                                                                                                                                     >  1:217834/1‑251 (MQ=255)                  taataaCCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCAc                                                                                                                                                                                                                              <  1:81121/251‑1 (MQ=255)                           ggAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCAc                                                                                                                                                                                                                              <  1:212863/242‑1 (MQ=255)                                  gCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCAc                                                                                                                                                                                                                              <  1:901582/235‑1 (MQ=255)                                   cACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATaa                                                                                                                                                                                                             <  1:195600/251‑1 (MQ=255)                                       cATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGcc                                                                                                                                                                                                         <  1:639860/251‑1 (MQ=255)                                            cATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGt                                                                                                                                                                                                    >  1:645500/1‑251 (MQ=255)                                              tataAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTAc                                                                                                                                                                                                  <  1:779266/251‑1 (MQ=255)                                                taAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGc                                                                                                                                                                                                <  1:252001/251‑1 (MQ=255)                                                            ggAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGa                                                                                                                                                                                    >  1:647183/1‑251 (MQ=255)                                                                          tGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATaa                                                                                                                                                                                                             <  1:723789/212‑1 (MQ=255)                                                                           ggTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACAt                                                                                                                                                                     >  1:893157/1‑251 (MQ=255)                                                                                         cTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTg                                                                                                                                                       <  1:313200/251‑1 (MQ=255)                                                                                           ttttGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATaa                                                                                                                                                                                                             >  1:724616/1‑195 (MQ=255)                                                                                           ttttGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGcc                                                                                                                                                                                                         >  1:30584/1‑199 (MQ=255)                                                                                           ttttGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTa                                                                                                                                                     >  1:530680/1‑251 (MQ=255)                                                                                              tGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAata                                                                                                                                                  <  1:747014/251‑1 (MQ=255)                                                                                                          gAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAatatttgatatttg                                                                                                                                       >  1:397581/1‑250 (MQ=255)                                                                                                          gAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAatatttgatatttg                                                                                                                                       <  1:244041/250‑1 (MQ=255)                                                                                                            aTCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGcc                                                                                                                                                                                                         <  1:123221/182‑1 (MQ=255)                                                                                                             tCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATc                                                                                                                                   <  1:747187/251‑1 (MQ=255)                                                                                                                   tCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGc                                                                                                                              >  1:832046/1‑250 (MQ=255)                                                                                                                           gAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAAtt                                                                                                                     >  1:517287/1‑251 (MQ=255)                                                                                                                                        cTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAatatttgatatt                                                                                                                                         <  1:377045/218‑1 (MQ=255)                                                                                                                                            aTTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTg                                                                                                                                                                                                     <  1:307960/154‑1 (MQ=255)                                                                                                                                                   tCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAAtgcg                                                                                              <  1:756851/250‑1 (MQ=255)                                                                                                                                                   tCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAAtg                                                                                                <  1:563736/248‑1 (MQ=255)                                                                                                                                                         catcaATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAAtgcggttgcgg                                                                                       >  1:461699/1‑251 (MQ=255)                                                                                                                                                           tcaATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTg                                                                                     >  1:859672/1‑251 (MQ=255)                                                                                                                                                              aTAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCCCCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAAttg                                                                                                                    >  1:428157/1‑217 (MQ=255)                                                                                                                                                              aTAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGt                                                                                  >  1:138643/1‑251 (MQ=255)                                                                                                                                                              aTAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGt                                                                                  >  1:54794/1‑251 (MQ=255)                                                                                                                                                                aaaCGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATc                                                                                                                                                                    >  1:573255/1‑167 (MQ=255)                                                                                                                                                                aaaCGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTg                                                                                >  1:623538/1‑251 (MQ=255)                                                                                                                                                                  aCGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAg                                                                                                                                                                                                           >  1:367400/1‑126 (MQ=255)                                                                                                                                                                  aCGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGgtgt                                                                               >  1:585306/1‑250 (MQ=255)                                                                                                                                                                     gTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACg                                                                                                           >  1:69738/1‑219 (MQ=255)                                                                                                                                                                        ttCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCa                                                                                                                                                                                                                                                <  1:717518/83‑1 (MQ=255)                                                                                                                                                                        ttCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAAt                                                                        <  1:473431/251‑1 (MQ=255)                                                                                                                                                                                 gTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCg                                                               >  1:754194/1‑251 (MQ=255)                                                                                                                                                                                             gTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGcc                                                                                                                                                                                                         >  1:7225/1‑101 (MQ=255)                                                                                                                                                                                                 ctgcGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCgg                                               <  1:33856/251‑1 (MQ=255)                                                                                                                                                                                                            ggCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCAc                                                           >  1:480308/1‑228 (MQ=255)                                                                                                                                                                                                             gCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATc                                                                       >  1:793250/1‑215 (MQ=255)                                                                                                                                                                                                                  cAATTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCAcc                                                                                                                                                                 <  1:890018/120‑1 (MQ=255)                                                                                                                                                                                                                   aaTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGaa                                       >  1:145826/1‑241 (MQ=255)                                                                                                                                                                                                                    aTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCACGTCGGATAATTCTTCCCTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGAATGCCGTCACg                             >  1:623688/1‑250 (MQ=255)                                                                                                                                                                                                                    aTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTc                                                                                                                                                                                                                                            <  1:802051/43‑1 (MQ=255)                                                                                                                                                                                                                    aTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGcc                                                                                                                                                                                                         >  1:703761/1‑78 (MQ=255)                                                                                                                                                                                                                    aTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTg                                                                                                                               >  1:294487/1‑152 (MQ=255)                                                                                                                                                                                                                    aTTTAATGTTGTTGCCGCTGTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGAATGCCGTCACGc                            <  1:191959/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAAt                                                                        >  1:746034/1‑188 (MQ=255)                                                                                                                                                                                                                                       gTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCAt                                                  <  1:784125/210‑1 (MQ=255)                                                                                                                                                                                                                                       gTTATCAATCACGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGAATGCCg                                  <  1:496261/226‑1 (MQ=255)                                                                                                                                                                                                                                              atcaCGCTTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCCCCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCCCCGCGACCATCGGTTCGCGATTGCCGTCGCGCCACATTTTGTTGGCGGCGGATTCCGc  <  1:688701/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TCATTGAAGTGGTTGATTAATAACCAGGAATCAGCACCCATCGCATATAAACGCATTAAGGAATAATCGTTATTGGTTTCGGTCGCCACTTTTTGATATTGCGCGGAATCCGTATCTTTAAAGAATGGAATATCGCTGAATTGCAGTCCGTTCATCAATAAACGGTATTCCGGACCGTTATTGGAAGAGTTGCTGCGTGAGCTGGCGTACAATTTAATGTTGTTGCCGCTGTTATCAATCACGCCTTTCAAGTCGGATAATTCTTCACTTTTCGCAACGATATAAAGCCCTTGTACGCTTTGTCCTTGGAGCGCAACGCCGACATCACCGGCTTGGTTGTAATATTTGATATTTGCATCGGTGCCGGCTAATTGTTGCCAACGCACATTAAATGCGGTTGCGGTGCGTTGTCCTAAATCATTTTGCGGCACCGCGACCATCGGTTCGCGAATGCCGTCACGCCACATTTTGTTGGCGGCGGATTCCGC  >  1/997202‑997689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑