Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	996,804	A→G	*87Q (TAA→CAA)	unknown ←	UPF0102 protein YraN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	996,804	0	A	G	100.0%	111.0 / NA	42	*87Q (TAA→CAA)	unknown	UPF0102 protein YraN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/12);  total (30/12)

ACTTTATTACCACGCAACAGGCAGGCAATCAGGTGTTGCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTATGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCTTTTTGTTCAAATC  >  1/996560‑997031                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     aCTTTATTACCACGCAACAGGCAGGCAATCAGGTGTTGCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTgccag                                                                                                                                                                                                                                >  1:213425/1‑251 (MQ=255)  cTTTATTACCACGCAACAGGCAGGCAATCAGGTGTTGCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTgccag                                                                                                                                                                                                                                >  1:62589/1‑250 (MQ=255)  cTTTATTACCACGCAACAGGCAGGCAATCAGGTGTTGCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTgccag                                                                                                                                                                                                                                >  1:825833/1‑250 (MQ=255)                                      gCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGccatcggagg                                                                                                                                                                                                        <  1:239407/238‑10 (MQ=255)                                              tCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAAt                                                                                                                                                                                    >  1:21470/1‑250 (MQ=255)                                                     tttGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCtt                                                                                                                                                                            >  1:321704/1‑251 (MQ=255)                                                             cTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGc                                                                                                                                                                    <  1:457997/251‑1 (MQ=255)                                                               gAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGcg                                                                                                                                                                   >  1:103637/1‑249 (MQ=255)                                                                                aaataTGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑Attt                                                                                                                                                >  1:144304/1‑251 (MQ=255)                                                                                       aGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGtt                                                                                                                                         >  1:546860/1‑251 (MQ=255)                                                                                        gTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGATGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCTGCATTCAGCCAGCGTCGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGACCCAAAATGATC‑ATTTTTACGTTg                                                                                                                                        >  1:161373/1‑251 (MQ=255)                                                                                                      cTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACaa                                                                                                                          <  1:839190/251‑1 (MQ=255)                                                                                                      cTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACaa                                                                                                                          <  1:635223/251‑1 (MQ=255)                                                                                                        cATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAAt                                                                                                                        >  1:372055/1‑251 (MQ=255)                                                                                                         atatAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATa                                                                                                                       >  1:360129/1‑251 (MQ=255)                                                                                                         atatAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATa                                                                                                                       >  1:444911/1‑251 (MQ=255)                                                                                                               aTCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATgg                                                                                                                 >  1:739303/1‑251 (MQ=255)                                                                                                                     gAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGa                                                                                                                    >  1:865731/1‑242 (MQ=255)                                                                                                                           gtttTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGc                                                                                                                                                                    >  1:35991/1‑189 (MQ=255)                                                                                                                               tGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACAt                                                                                                 <  1:419692/251‑1 (MQ=255)                                                                                                                                     gTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGt                                                                                                                                                                                                                         <  1:524985/126‑1 (MQ=255)                                                                                                                                     gTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATgg                                                                                                                 >  1:430980/1‑229 (MQ=255)                                                                                                                                     gTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTaa                                                                                           >  1:834545/1‑251 (MQ=255)                                                                                                                                        aaaGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAAt                                                                                         <  1:120046/250‑1 (MQ=255)                                                                                                                                        aaaGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATc                                                                                        <  1:416427/251‑1 (MQ=255)                                                                                                                                                          aaGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTAttt                                                                                                            >  1:278949/1‑213 (MQ=255)                                                                                                                                                            gTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑Atttt                                                                                                                                               <  1:596829/176‑1 (MQ=255)                                                                                                                                                             ttGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGaaa                                                                     <  1:421000/249‑1 (MQ=255)                                                                                                                                                                     ccATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACaa                                                                                                                          >  1:389175/1‑188 (MQ=255)                                                                                                                                                                     ccATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTttggttg                                                            >  1:717170/1‑250 (MQ=255)                                                                                                                                                                              gTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAGGT‑CTGCCGGATTCAGGCATCTTTGATGGTTTTGGCAATCTTCGCTTTCCACGGCAGGTCCAAAATGATCAATTTTTCCG‑TGGCTCACTTCCACCAATACCATTGGATTTTGGTCCAAAATAATTAAATCCAATTCGGCCCCCTTGAAATTTTTGTTGGCGGCCAGaaa                                                 >  1:276079/1‑251 (MQ=255)                                                                                                                                                                              gTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCtt                                                                                                                                                                                            >  1:896484/1‑114 (MQ=255)                                                                                                                                                                                       ggTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACaa                                                                                                                          >  1:750009/1‑170 (MQ=255)                                                                                                                                                                                       ggTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATg                                          >  1:874181/1‑250 (MQ=255)                                                                                                                                                                                        gTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGc                                         >  1:889754/1‑250 (MQ=255)                                                                                                                                                                                        gTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGc                                         >  1:608480/1‑250 (MQ=255)                                                                                                                                                                                        gTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑A‑TTTTACGGTGGCGCACTTCCACAAATACGATGGGATTTTGTTCCAACa                                                                                                  >  1:157688/1‑192 (MQ=255)                                                                                                                                                                                               cAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGAt                                 <  1:656361/251‑1 (MQ=255)                                                                                                                                                                                                                     gCAATCGGTGTCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCttttt           >  1:188626/1‑251 (MQ=255)                                                                                                                                                                                                                              gtCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCTTTTTGTTCAAAt   >  1:149436/1‑250 (MQ=255)                                                                                                                                                                                                                              gtCTTCTAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCTTTTTGTTCAAAt   >  1:97851/1‑250 (MQ=255)                                                                                                                                                                                                                               tcttctAAACTTTGTTCCCGTTGTGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCTTTTTGTTCAAAtc  <  1:665067/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     ACTTTATTACCACGCAACAGGCAGGCAATCAGGTGTTGCGTCGCTTCGACGATTTGTGCGCTGAGCAGGCTTTTTGAATAAATATGAGTTTTAATATTTTCCTCATATAAATCATTGAGTTTGTTTTGCATAGTGAAAGAATATTAATCAAGAAAGTTGGAGATCCATTGAATGTCTTGATCGGTTTTACCAAACGCGACCAAATCGAAACGGCAATCGGTGTCTTCTAAACTTTGTTCCCGTTATGCCAGCCAAAGTGCTGCCGCATTCAGCCAGCGTTGTTGCTTTTGCCAATCTACGCTTTCCACGGCAGAGCCAAAATGATC‑ATTTTTACGTTGGCGCACTTCCACAAATACGATGGTATTTTGTTCCAACATAATTAAATCCAGTTCGCCGCACTTGAAATTTTGGTTGGCGGCGAGAAATTTCATGCCTTTAGATTCTAAAAAGAGGCGAGCTTTTTGTTCAAATC  >  1/996560‑997031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑