Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	993,070	T→C	L99L (CTA→CTG)	unknown ←	Cytochrome c‑type biogenesis protein CcdA homolog, associated with MetSO reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	993,070	0	T	C	100.0%	185.8 / NA	64	L99L (CTA→CTG)	unknown	Cytochrome c‑type biogenesis protein CcdA homolog, associated with MetSO reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/29);  total (35/29)

TAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCTAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGACAAAAAGG  >  1/992833‑993314                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     tAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAAt                                                                                                                                                                                                                                          <  1:763627/250‑1 (MQ=255)                       aagagTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATgg                                                                                                                                                                                                                     <  1:7232/249‑1 (MQ=255)                           gTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCt                                                                                                                                                                                                                              <  1:465854/236‑1 (MQ=255)                           gTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATgg                                                                                                                                                                                                                     <  1:678164/245‑1 (MQ=255)                                     cGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCa                                                                                                                                                                                                     <  1:365039/251‑1 (MQ=255)                                                     aCATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTTCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCaat                                                                                                                                                                                     <  1:430496/251‑1 (MQ=255)                                                                    atAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTAt                                                                                                                                                                       >  1:626162/1‑250 (MQ=255)                                                                     tAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAAtt                                                                                                                                                                                                                          <  1:249889/198‑1 (MQ=255)                                                                          gCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTaaa                                                                                                                                                                >  1:462664/1‑251 (MQ=255)                                                                            aCCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGa                                                                                                                                                              <  1:674730/251‑1 (MQ=255)                                                                               gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGAt                                                                                                                                                                                                 >  1:479943/1‑213 (MQ=255)                                                                               gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                                           >  1:805417/1‑251 (MQ=255)                                                                               gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                                           >  1:131658/1‑251 (MQ=255)                                                                               gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                                           >  1:595463/1‑251 (MQ=255)                                                                               gTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATa                                                                                                                                                           >  1:755430/1‑251 (MQ=255)                                                                                    gagCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATGCAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCATTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCaataa                                                                                                                                                                                   <  1:248999/222‑1 (MQ=255)                                                                                      gCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGc                                                                                                                                                     >  1:385909/1‑250 (MQ=255)                                                                                                             cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATAc                                                                                                                                                                                                                                >  1:639583/1‑152 (MQ=255)                                                                                                             cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCCGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTa                                                                                                                             >  1:442052/1‑251 (MQ=255)                                                                                                             cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATa                                                                                                                                                                                                <  1:228972/184‑1 (MQ=255)                                                                                                             cGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATc                                                                                                                                            <  1:517070/236‑1 (MQ=255)                                                                                                               ccAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAgg                                                                                                                           <  1:515793/251‑1 (MQ=255)                                                                                                                  gAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATg                                                                                                                                                                                 <  1:505848/194‑1 (MQ=255)                                                                                                                        aTGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATTCCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                                                    <  1:208938/249‑1 (MQ=255)                                                                                                                        aTGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAg                                                                                                                  <  1:804925/251‑1 (MQ=255)                                                                                                                          gCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGt                                                                                                                >  1:3510/1‑251 (MQ=255)                                                                                                                                    gTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                                                    >  1:108122/1‑237 (MQ=255)                                                                                                                                    gTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATaaa                                                                                                      >  1:698078/1‑251 (MQ=255)                                                                                                                                        aaTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTc                                                                                                                                                                                                                 <  1:867622/140‑1 (MQ=255)                                                                                                                                        aaTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGAt                                                                                                         >  1:78439/1‑244 (MQ=255)                                                                                                                                         aTACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGt                                                                                                                <  1:463287/236‑1 (MQ=255)                                                                                                                                           aCAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCaa                                                                                               <  1:358026/251‑1 (MQ=255)                                                                                                                                                gtgtCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGc                                                                                                                                                                                            <  1:406188/153‑1 (MQ=255)                                                                                                                                                  gtCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACaa                                                                                        >  1:666404/1‑251 (MQ=255)                                                                                                                                                        ccAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAggc                                                                                  <  1:308279/251‑2 (MQ=255)                                                                                                                                                           aGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAAtt                                                                                                                                                       >  1:719636/1‑179 (MQ=255)                                                                                                                                                            ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAAt                                                                                                                                                        <  1:646822/177‑1 (MQ=255)                                                                                                                                                            ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGa                                                                                                                    >  1:154482/1‑213 (MQ=255)                                                                                                                                                            ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACaaaaa                                                                                     <  1:117058/244‑1 (MQ=255)                                                                                                                                                            ggCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAgg                                                                                   <  1:812558/246‑1 (MQ=255)                                                                                                                                                             gCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGaa                                                                                                                                                                                                           <  1:212730/125‑1 (MQ=255)                                                                                                                                                                gAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGa                                                                                                          <  1:706982/219‑1 (MQ=255)                                                                                                                                                                gAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATaa                                                                          >  1:201167/1‑251 (MQ=255)                                                                                                                                                                  aGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATg                                                                                                                                                                                 >  1:138680/1‑146 (MQ=255)                                                                                                                                                                  aGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGcc                                                                                                                               >  1:472216/1‑196 (MQ=255)                                                                                                                                                                   ggTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTAACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAAttt                                                                       >  1:215570/1‑251 (MQ=255)                                                                                                                                                                        gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCg                                                                                                                                                                     <  1:162642/152‑1 (MQ=255)                                                                                                                                                                        gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTa                                                                  >  1:873809/1‑251 (MQ=255)                                                                                                                                                                        gCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTa                                                                  >  1:235894/1‑251 (MQ=255)                                                                                                                                                                               gAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAAc                                                                                                     <  1:866549/209‑1 (MQ=255)                                                                                                                                                                               gAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCtt                                                           >  1:638989/1‑251 (MQ=255)                                                                                                                                                                                            aaaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACg                                                                                                                     >  1:170449/1‑180 (MQ=255)                                                                                                                                                                                            aaaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGcc                                              <  1:837812/251‑1 (MQ=255)                                                                                                                                                                                             aaGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCaa                                                                                                                                                                                                                                           <  1:852334/61‑1 (MQ=255)                                                                                                                                                                                                     cTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAAcc                                                                                                    >  1:461713/1‑188 (MQ=255)                                                                                                                                                                                                     cTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGaaataaat                                     >  1:877462/1‑251 (MQ=255)                                                                                                                                                                                                       tttGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCg                                   >  1:417415/1‑251 (MQ=255)                                                                                                                                                                                                                 gTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAAc                                                                                          >  1:851346/1‑186 (MQ=255)                                                                                                                                                                                                                 gTTTTTACTTCAACTACTTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATggg                         >  1:128471/1‑251 (MQ=255)                                                                                                                                                                                                                                actTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGa          >  1:194538/1‑251 (MQ=255)                                                                                                                                                                                                                                actTTGGTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGa          >  1:18226/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGt                                                                                                                                                                    >  1:600725/1‑91 (MQ=255)                                                                                                                                                                                                                                      gTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAgg                                                                                   >  1:70567/1‑172 (MQ=255)                                                                                                                                                                                                                                      gTTCTTTCCAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCAt                                                                            >  1:404301/1‑179 (MQ=255)                                                                                                                                                                                                                                        tctttccAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAGAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGACAAAAagg  <  1:422890/251‑1 (MQ=255)                                                                                                                                                                                                                                        tctttccAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGACAAAAagg  <  1:778791/251‑1 (MQ=255)                                                                                                                                                                                                                                        tctttccAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGACAAAAagg  <  1:358046/251‑1 (MQ=255)                                                                                                                                                                                                                                         ctttccAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGa                                            <  1:848664/208‑1 (MQ=255)                                                                                                                                                                                                                                         ctttccAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAgg             <  1:673080/239‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     TAACTCTTGTGAGAATAAAGAGAAGAGTACAAACGGCGTTGCCAAACCTAATACATAAACCAACATCATAGAAGCACCGTATAGAGCGGAACCTTCATCGCCGGACAACGCCAGAACAGATGCTAAAATCGGTCCAATACAAGGTGTCCAGCCAAGGCTGAAGGTAAGCCCTAGGAAAAAGGCTTCAAAAGAGGCACTTTTGCCTTCAGTTTTTACTTCAACTACTTTGGTTCTTTCTAGGAAATTCAATTTGATAATACCTAATTGATGGATTCCCAGAATAATCACGATAATGCCGGCAATAATGCGCACGTTATCGTTAAAGAATAAATTGCCTAAAAATCCGAAGCTGAAGCCTAGGCTGACGAAGGTTAAAGATAAACCTGCAATGAACAAAAAGGTGTTCATAATTTTTCTACCGCCTTTCGCCAAGATGCCGAAATAAATCGGGATAATGGGGAAAATGCAAGGCGACAAAAAGG  >  1/992833‑993314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑