Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	991,367	T→C	M1M (ATG→GTG) †	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	991,367	0	T	C	100.0%	23.4 / NA	14	M1M (ATG→GTG) †	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/14);  total (0/14)

TATTGCCCTTGATAGTTGAGGTTTGTCTTAATCGTAATTCTTTGGGCGCCATCAAATGCCTGCCAGTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACATAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCA  >  1/991123‑991452                                                                                                                                                                                                                                                     |                                                                                      tatTGCCCTTGATAGTTGAGGTTTGTCTTAATCGTAATTCTTTGGGCGCCATCAAATGCCTGCCAGTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCGCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATAc                                                                                 <  1:822005/251‑1 (MQ=37)                                                 ccATCAAATGCCTGCCAGTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTc                                 <  1:578119/251‑1 (MQ=18)                                                                  gTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTc                                 <  1:383881/234‑1 (MQ=17)                                                                  gTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTc                                 <  1:786596/234‑1 (MQ=17)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACGCAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:496910/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:416003/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:46835/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:528193/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:547462/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:739613/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:788928/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:810133/250‑1 (MQ=14)                                                                   ttATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                <  1:894489/250‑1 (MQ=14)                                                                                cTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCa  <  1:341697/251‑1 (MQ=11)                                                                                                                                                                                                                                                     |                                                                                      TATTGCCCTTGATAGTTGAGGTTTGTCTTAATCGTAATTCTTTGGGCGCCATCAAATGCCTGCCAGTTATTGACTTTATCTTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACATAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCA  >  1/991123‑991452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑