Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	983,852	T→C	*321Q (TAA→CAA)	unknown →	Cys regulon transcriptional activator CysB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	983,852	0	T	C	100.0%	129.6 / NA	46	*321Q (TAA→CAA)	unknown	Cys regulon transcriptional activator CysB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

GGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGTGGTGAAAAGATTATTTGATGACGTCAGATTGTAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑CCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGAC  >  1/983606‑984064                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                             ggTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCaaa                                                                                                                                                                                                                           >  1:184710/1‑250 (MQ=255)       ggCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTa                                                                                                                                                                                                                    >  1:117563/1‑251 (MQ=255)        gCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTaa                                                                                                                                                                                                                   >  1:464236/1‑251 (MQ=255)           ttATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTg                                                                                                                                                                                                                >  1:741424/1‑251 (MQ=255)             aTGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGaa                                                                                                                                                                                                              <  1:198974/251‑1 (MQ=255)                                        gATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTACGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTg                                                                                                                                                                                   >  1:409637/1‑251 (MQ=255)                                                        cACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑cc‑‑c                                                                                                                                                                >  1:523159/1‑250 (MQ=255)                                                        cACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑cc‑‑c                                                                                                                                                                >  1:111759/1‑250 (MQ=255)                                                                gCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑Cg                                                                                                                                                               <  1:577010/243‑1 (MQ=255)                                                                    aTCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccccccccctt                                                                                                                                                  <  1:365144/250‑5 (MQ=255)                                                                      cATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑cccccccccttttt                                                                                                                                               >  1:867162/1‑244 (MQ=255)                                                                      cATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccc                                                                                                                                                          <  1:522670/240‑1 (MQ=255)                                                                             tCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACa                                                                                                                                          <  1:642887/251‑1 (MQ=255)                                                                                      gCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAg                                                                                                                                 >  1:613909/1‑251 (MQ=255)                                                                                       cATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAgg                                                                                                                                <  1:556314/251‑1 (MQ=255)                                                                                       cATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAg                                                                                                                                 <  1:383449/250‑1 (MQ=255)                                                                                        aTGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                              <  1:187663/251‑1 (MQ=255)                                                                                        aTGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                              <  1:672866/251‑1 (MQ=255)                                                                                                                      ttCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTTACATTTATATAGG‑AAGCAAAATGTCAAAATTACaacaacctga                                                                                                 >  1:69374/1‑246 (MQ=255)                                                                                                                           gCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATTTTGG‑AAGCAAAAAGACAACTTTATAACAAC‑ATATTTaaaa                                                                                          >  1:292920/1‑251 (MQ=255)                                                                                                                              tAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                              <  1:683959/213‑1 (MQ=255)                                                                                                                               aaTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCt                                                                                      <  1:533749/251‑1 (MQ=255)                                                                                                                                aTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAACATTTCTATATG‑AAGCAAAATGTAAAATTTAAAAAAAC‑TTATTgttgtttctt                                                                                     >  1:351842/1‑242 (MQ=255)                                                                                                                                aTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTTATATTGG‑a                                                                                                                              >  1:421314/1‑211 (MQ=255)                                                                                                                                   atatGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAACATTTATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTGAAAACGCttttt                                                                                  >  1:777274/1‑250 (MQ=255)                                                                                                                                      tgtatgATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTg                                                                                              <  1:688084/236‑1 (MQ=255)                                                                                                                                                                   cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CCCACC‑‑CCCCCCCCTTTTACTTACCTTAATATAGGAAAACAAAA‑ATCAAAATTACAACAACTTTATT‑AAAACTCTATTGAAAAACTCACTACAATTACACCAAAAAcccat                                                  >  1:328806/1‑248 (MQ=255)                                                                                                                                                                   cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTa                                                                <  1:398688/237‑1 (MQ=255)                                                                                                                                                                   cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCCTTTTACTAAAATTGATATAGG‑AAGAAAAACAACAAATTTAAAACAAC‑TTATTTAAAAAGATTTTGaaa                                                                               >  1:95225/1‑222 (MQ=255)                                                                                                                                                                     cTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGa                                                <  1:487425/251‑1 (MQ=255)                                                                                                                                                                            cGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACaa                                         <  1:253814/251‑1 (MQ=255)                                                                                                                                                                                aaaaCGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAAcc                                      <  1:770802/250‑1 (MQ=255)                                                                                                                                                                                       ggAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTTACATTTATATAg                                                                                                                                 >  1:290236/1‑154 (MQ=255)                                                                                                                                                                                             ggCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTTATATAGG‑AAGCAAAATGACAAATTTACAACCAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGcggcgggtgg                        >  1:140676/1‑247 (MQ=255)                                                                                                                                                                                                   gCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAac                                                                                                                                                                               >  1:95756/1‑100 (MQ=255)                                                                                                                                                                                                   gCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACACCCC‑‑CC‑‑CCCCCCCCTTTTTCTCCTACTTATATAGT‑ATGCAAAAAGTCAAATTTACAACACC‑ATTTTTCAAACGCCTTTTAAAAAAGCAATTAAAATACACCAAAAACCACTCACGTACAAAcccccctcttttaaacacat                    >  1:133774/1‑232 (MQ=255)                                                                                                                                                                                                     gTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAggggg                >  1:29933/1‑248 (MQ=255)                                                                                                                                                                                                          cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACACCCC‑‑CC‑‑CCCCCCCCTTTTTCTCACATTTATATAGG‑AAGCAAAAAGCCCAATTTACAACAAC‑TTATTTAAAAAGCTTTTGTAAAAAACACTGAAATTACACCAAAAAACATTTACGTACAAAACACTGCGGCGGTTGTAGAggagaattaa           >  1:244908/1‑243 (MQ=255)                                                                                                                                                                                                          cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAAAATTGATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTTAAAAAGATTTTTTAAAAAGCACTGAAATTAAACCAAAAAAAATTTACGAACAAAATCGTGCGGCGGTTGaaaggggggttgaa           >  1:221628/1‑239 (MQ=255)                                                                                                                                                                                                          cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGaa           <  1:536102/251‑1 (MQ=255)                                                                                                                                                                                                            cGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAgg         <  1:806928/251‑1 (MQ=255)                                                                                                                                                                                                            cGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCAATTACGTACAAACCCGTGCGGCGGTTGAAGAGGGGATTGaaag         >  1:622811/1‑249 (MQ=255)                                                                                                                                                                                                              aCAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccccc                                                                                                                                                        <  1:37799/106‑1 (MQ=255)                                                                                                                                                                                                                 ataatGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTTAAAACGCTTTTGAAAAAAGCACTGAAATTACAACAAAAACAATTAACGTACAAACACATGCGGCGGGTGAAGAAGGGATTGAAGGCTTgg    >  1:373522/1‑251 (MQ=255)                                                                                                                                                                                                                 ataatGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCCTTTTTCTTACATTTCTATAGG‑AAGAAAAATGTTAAATTTACAACAAC‑TTATTTAAAAAGCTTTTTTAAAACGCACTGAAAATACACCAAAAAACATTGACGTACAAACCCAAGCCGCGGTTGGAGGGGGGATAGAAGGCtgtt    >  1:448571/1‑248 (MQ=255)                                                                                                                                                                                                                                             cGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGC‑CCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGAc  <  1:343970/224‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                             GGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGTGGTGAAAAGATTATTTGATGACGTCAGATTGTAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑CCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGAC  >  1/983606‑984064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑