Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	983,820	T→C	V310A (GTG→GCG)	unknown →	Cys regulon transcriptional activator CysB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	983,820	0	T	C	100.0%	140.4 / NA	50	V310A (GTG→GCG)	unknown	Cys regulon transcriptional activator CysB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/20);  total (30/20)

TCAAAACCTATGTCCGTTTAGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGTGGTGAAAAGATTATTTGATGACGTCAGATTGTAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑CCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGG  >  1/983580‑984062                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           tCAAAACCTATGTCCGTTTAGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGa                                                                                                                                                                                                                                                  <  1:191313/251‑1 (MQ=255)               cGTTTAGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGt                                                                                                                                                                                                                                    >  1:762555/1‑251 (MQ=255)                    aGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCTCCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGAt                                                                                                                                                                                                                               >  1:791035/1‑251 (MQ=255)                    aGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGAt                                                                                                                                                                                                                               >  1:85310/1‑251 (MQ=255)                      gCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTg                                                                                                                                                                                                                             >  1:271082/1‑251 (MQ=255)                           ggTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCaaa                                                                                                                                                                                                                         >  1:184710/1‑250 (MQ=255)                                 ggCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTa                                                                                                                                                                                                                  >  1:117563/1‑251 (MQ=255)                                  gCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTaa                                                                                                                                                                                                                 >  1:464236/1‑251 (MQ=255)                                     ttATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTg                                                                                                                                                                                                              >  1:741424/1‑251 (MQ=255)                                       aTGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGaa                                                                                                                                                                                                            <  1:198974/251‑1 (MQ=255)                                                                  gATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTACGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTg                                                                                                                                                                                 >  1:409637/1‑251 (MQ=255)                                                                                  cACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑cc‑‑c                                                                                                                                                              >  1:523159/1‑250 (MQ=255)                                                                                  cACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑cc‑‑c                                                                                                                                                              >  1:111759/1‑250 (MQ=255)                                                                                          gCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑Cg                                                                                                                                                             <  1:577010/243‑1 (MQ=255)                                                                                              aTCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccccccccctt                                                                                                                                                <  1:365144/250‑5 (MQ=255)                                                                                                cATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑cccccccccttttt                                                                                                                                             >  1:867162/1‑244 (MQ=255)                                                                                                cATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccc                                                                                                                                                        <  1:522670/240‑1 (MQ=255)                                                                                                       tCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACa                                                                                                                                        <  1:642887/251‑1 (MQ=255)                                                                                                                gCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAg                                                                                                                               >  1:613909/1‑251 (MQ=255)                                                                                                                 cATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAgg                                                                                                                              <  1:556314/251‑1 (MQ=255)                                                                                                                 cATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAg                                                                                                                               <  1:383449/250‑1 (MQ=255)                                                                                                                  aTGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                            <  1:187663/251‑1 (MQ=255)                                                                                                                  aTGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                            <  1:672866/251‑1 (MQ=255)                                                                                                                                                ttCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTTACATTTATATAGG‑AAGCAAAATGTCAAAATTACaacaacctga                                                                                               >  1:69374/1‑246 (MQ=255)                                                                                                                                                     gCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATTTTGG‑AAGCAAAAAGACAACTTTATAACAAC‑ATATTTaaaa                                                                                        >  1:292920/1‑251 (MQ=255)                                                                                                                                                        tAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑a                                                                                                                            <  1:683959/213‑1 (MQ=255)                                                                                                                                                         aaTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCt                                                                                    <  1:533749/251‑1 (MQ=255)                                                                                                                                                          aTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAACATTTCTATATG‑AAGCAAAATGTAAAATTTAAAAAAAC‑TTATTgttgtttctt                                                                                   >  1:351842/1‑242 (MQ=255)                                                                                                                                                          aTTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTTATATTGG‑a                                                                                                                            >  1:421314/1‑211 (MQ=255)                                                                                                                                                             atatGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAACATTTATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTGAAAACGCttttt                                                                                >  1:777274/1‑250 (MQ=255)                                                                                                                                                                tgtatgATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTg                                                                                            <  1:688084/236‑1 (MQ=255)                                                                                                                                                                                             cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CCCACC‑‑CCCCCCCCTTTTACTTACCTTAATATAGGAAAACAAAA‑ATCAAAATTACAACAACTTTATT‑AAAACTCTATTGAAAAACTCACTACAATTACACCAAAAAcccat                                                >  1:328806/1‑248 (MQ=255)                                                                                                                                                                                             cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTa                                                              <  1:398688/237‑1 (MQ=255)                                                                                                                                                                                             cACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCCTTTTACTAAAATTGATATAGG‑AAGAAAAACAACAAATTTAAAACAAC‑TTATTTAAAAAGATTTTGaaa                                                                             >  1:95225/1‑222 (MQ=255)                                                                                                                                                                                               cTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGa                                              <  1:487425/251‑1 (MQ=255)                                                                                                                                                                                                      cGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACaa                                       <  1:253814/251‑1 (MQ=255)                                                                                                                                                                                                          aaaaCGTGGAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAAcc                                    <  1:770802/250‑1 (MQ=255)                                                                                                                                                                                                                 ggAACGGGCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTTACATTTATATAg                                                                                                                               >  1:290236/1‑154 (MQ=255)                                                                                                                                                                                                                       ggCAGAGCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTTATATAGG‑AAGCAAAATGACAAATTTACAACCAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGcggcgggtgg                      >  1:140676/1‑247 (MQ=255)                                                                                                                                                                                                                             gCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAac                                                                                                                                                                             >  1:95756/1‑100 (MQ=255)                                                                                                                                                                                                                             gCGTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACACCCC‑‑CC‑‑CCCCCCCCTTTTTCTCCTACTTATATAGT‑ATGCAAAAAGTCAAATTTACAACACC‑ATTTTTCAAACGCCTTTTAAAAAAGCAATTAAAATACACCAAAAACCACTCACGTACAAAcccccctcttttaaacacat                  >  1:133774/1‑232 (MQ=255)                                                                                                                                                                                                                               gTATGCACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAggggg              >  1:29933/1‑248 (MQ=255)                                                                                                                                                                                                                                    cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACACCCC‑‑CC‑‑CCCCCCCCTTTTTCTCACATTTATATAGG‑AAGCAAAAAGCCCAATTTACAACAAC‑TTATTTAAAAAGCTTTTGTAAAAAACACTGAAATTACACCAAAAAACATTTACGTACAAAACACTGCGGCGGTTGTAGAggagaattaa         >  1:244908/1‑243 (MQ=255)                                                                                                                                                                                                                                    cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTTCTAAAATTGATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTTAAAAAGATTTTTTAAAAAGCACTGAAATTAAACCAAAAAAAATTTACGAACAAAATCGTGCGGCGGTTGaaaggggggttgaa         >  1:221628/1‑239 (MQ=255)                                                                                                                                                                                                                                    cACGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGaa         <  1:536102/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAgg       <  1:806928/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGACAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCAATTACGTACAAACCCGTGCGGCGGTTGAAGAGGGGATTGaaag       >  1:622811/1‑249 (MQ=255)                                                                                                                                                                                                                                        aCAATAATGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑ccccc                                                                                                                                                      <  1:37799/106‑1 (MQ=255)                                                                                                                                                                                                                                           ataatGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAAAAAC‑TTATTTAAAACGCTTTTGAAAAAAGCACTGAAATTACAACAAAAACAATTAACGTACAAACACATGCGGCGGGTGAAGAAGGGATTGAAGGCTTgg  >  1:373522/1‑251 (MQ=255)                                                                                                                                                                                                                                           ataatGCGGTGAAAAGATTATTTGATGACGTCAGATTGCAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CGCCCCCCCCCCTTTTTCTTACATTTCTATAGG‑AAGAAAAATGTTAAATTTACAACAAC‑TTATTTAAAAAGCTTTTTTAAAACGCACTGAAAATACACCAAAAAACATTGACGTACAAACCCAAGCCGCGGTTGGAGGGGGGATAGAAGGCtgtt  >  1:448571/1‑248 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           TCAAAACCTATGTCCGTTTAGGCTTAGGTGTGGGCATTATGGCGTCCATGGCGCATACGCCGGCGGATGTTGATTTGGTGGCACTTAAAGCGGATCATTTATTCCGCGCCAGCATGACGCAAATCGCCTTTAAACACGGCGCTTTCCTGCGTAATTATATGTATGATTTTATCAACTATTTTTCACCGCACTTAACCCGTGAAAACGTGGAACGGGCAGAGCGTATGCACGACAATAATGTGGTGAAAAGATTATTTGATGACGTCAGATTGTAAACTTTGTAATTGAAAAGTTGGCTTTTCGCACATTGAATTTGCAACGACGAACA‑‑CC‑‑CG‑‑CCCCCCCTTTTTACTAACATTGATATAGG‑AAGCAAAATGTCAAATTTACAACAAC‑TTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGG  >  1/983580‑984062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑