Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	976,846	A→C	I427L (ATT→CTT)	unknown →	Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) @ Dihydrolipoamide dehydrogenase (EC 1.8.1.4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	976,846	0	A	C	100.0%	242.2 / NA	87	I427L (ATT→CTT)	unknown	Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) @ Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/42);  total (45/42)

CGTCAATCGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTATTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGCTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACTTTATCAAACAAATAAAGTG  >  1/976610‑977092                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       cGTCAATCGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATg                                                                                                                                                                                                                                          >  1:250590/1‑251 (MQ=255)    cAATCGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGt                                                                                                                                                                                                                                       <  1:797182/251‑1 (MQ=255)        cGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTtgtg                                                                                                                                                                                                                                   >  1:649795/1‑251 (MQ=255)        cGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTtgtg                                                                                                                                                                                                                                   >  1:474768/1‑251 (MQ=255)          cTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGa                                                                                                                                                                                                                                  >  1:131587/1‑250 (MQ=255)          cTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGa                                                                                                                                                                                                                                  <  1:512422/250‑1 (MQ=255)          cTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGa                                                                                                                                                                                                                                  <  1:328610/250‑1 (MQ=255)           ttACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGAcg                                                                                                                                                                                                                                <  1:146930/251‑1 (MQ=255)                    gCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACa                                                                                                                                                                                                                       >  1:87496/1‑251 (MQ=255)                      cGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACAt                                                                                                                                                                                                                      >  1:651381/1‑250 (MQ=255)                        gAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGa                                                                                                                                                                                                                                              >  1:99864/1‑224 (MQ=255)                        gAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGt                                                                                                                                                                                                                                       >  1:601445/1‑231 (MQ=255)                        gAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCg                                                                                                                                                                                                                    >  1:585327/1‑250 (MQ=255)                        gAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCg                                                                                                                                                                                                                    >  1:497238/1‑250 (MQ=255)                         aaGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGc                                                                                                                                                                                                                   <  1:107055/250‑1 (MQ=255)                          aGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCAt                                                                                                                                                                                                                 >  1:350620/1‑251 (MQ=255)                                   ggtaggtaAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATc                                                                                                                                                                                                         >  1:608379/1‑250 (MQ=255)                                     taggtaAAACCGAGCAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCTTCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCa                                                                                                                                                                                                       <  1:519440/250‑1 (MQ=255)                                       ggtaAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACg                                                                                                                                                                                                     >  1:698978/1‑250 (MQ=255)                                         taAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGcc                                                                                                                                                                                                   <  1:4067/250‑1 (MQ=255)                                                       aaTGTAAAGCCGAAGGCGTAATCTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTAACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTacac                                                                                                                                                                                    <  1:421832/251‑1 (MQ=255)                                                       aaTGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTacac                                                                                                                                                                                    <  1:140379/251‑1 (MQ=255)                                                       aaTGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTaca                                                                                                                                                                                     >  1:861232/1‑250 (MQ=255)                                                        aTGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACttt                                                                                                                                                                                        <  1:461179/246‑1 (MQ=255)                                                        aTGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACg                                                                                                                                                                                   >  1:394406/1‑251 (MQ=255)                                                        aTGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAAGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTacac                                                                                                                                                                                    >  1:424389/1‑250 (MQ=255)                                                          gTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACt                                                                                                                                                                                                            >  1:690432/1‑224 (MQ=255)                                                            aaaggcggaGGCCTAAACTACGAAAGCCCCAACCTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATc                                                                                                                                                                               <  1:663786/243‑1 (MQ=255)                                                                     ggCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATcc                                                                                                                                                                                                        >  1:755093/1‑217 (MQ=255)                                                                      gCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCtt                                                                                                                                                                     >  1:386146/1‑251 (MQ=255)                                                                           aaCTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTACCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCgg                                                                                                                                                                 >  1:804912/1‑250 (MQ=255)                                                                                    aTCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGcc                                                                                                                                                                                                   >  1:39961/1‑207 (MQ=255)                                                                                    aTCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGcc                                                                                                                                                                                                   >  1:69919/1‑207 (MQ=255)                                                                                      cGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTg                                                                                                                                                     >  1:8836/1‑251 (MQ=255)                                                                                        cAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAg                                                                                                                                                   <  1:469553/251‑1 (MQ=255)                                                                                        cAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAg                                                                                                                                                   <  1:822289/251‑1 (MQ=255)                                                                                            cTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGcc                                                                                                                                                                                                   >  1:38835/1‑199 (MQ=255)                                                                                                 cATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGggtcggtc                                                                                                                                          <  1:410588/251‑1 (MQ=255)                                                                                                     ggCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCg                                                                                                                                                                          <  1:391544/215‑1 (MQ=255)                                                                                                          cTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGAc                                                                                                                                    <  1:589609/248‑1 (MQ=255)                                                                                                             ccGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCt                                                                                                                              <  1:601197/251‑1 (MQ=255)                                                                                                                  cGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATcc                                                                                                                         <  1:701829/251‑1 (MQ=255)                                                                                                                       aaTCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAg                                                                                                                    <  1:463456/251‑1 (MQ=255)                                                                                                                       aaTCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAg                                                                                                                    <  1:757346/251‑1 (MQ=255)                                                                                                                           gCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAg                                                                                                                                                   >  1:163165/1‑216 (MQ=255)                                                                                                                                            cGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAg                                                                                                                                                   <  1:884552/199‑1 (MQ=255)                                                                                                                                            cGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCt                                                                                                                              >  1:379036/1‑220 (MQ=255)                                                                                                                                            cGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTgttt                                                                                               >  1:876518/1‑248 (MQ=255)                                                                                                                                            cGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTgttt                                                                                               >  1:138404/1‑248 (MQ=255)                                                                                                                                             ggTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATcc                                                                                                                         >  1:363405/1‑224 (MQ=255)                                                                                                                                             ggTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTgttt                                                                                               >  1:576669/1‑247 (MQ=255)                                                                                                                                              gTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCa                                                                                             >  1:570221/1‑251 (MQ=255)                                                                                                                                                        aCTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGAc                                                                                                                                    >  1:9227/1‑202 (MQ=255)                                                                                                                                                             ttttCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGcc                                                                              <  1:331080/251‑1 (MQ=255)                                                                                                                                                                 cGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCttt                                                                           >  1:855221/1‑250 (MQ=255)                                                                                                                                                                 cGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTc                                                                          <  1:273692/251‑1 (MQ=255)                                                                                                                                                                   aTAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGc                                                                                                                   <  1:400966/208‑1 (MQ=255)                                                                                                                                                                     aaaGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAaataa                                                                                                      <  1:67054/219‑1 (MQ=255)                                                                                                                                                                        gACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTggg                                                                   >  1:15888/1‑251 (MQ=255)                                                                                                                                                                        gACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTggg                                                                   >  1:852166/1‑251 (MQ=255)                                                                                                                                                                              cATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGt                                                                                                                                                                            <  1:804024/140‑1 (MQ=255)                                                                                                                                                                              cATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAgg                                                               >  1:893705/1‑249 (MQ=255)                                                                                                                                                                                   cgTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTAt                                                        <  1:674239/251‑1 (MQ=255)                                                                                                                                                                                   cgTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTAt                                                        <  1:585864/251‑1 (MQ=255)                                                                                                                                                                                    gTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATCGGTTGTGACGCGGTAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGCGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGATAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTAtt                                                       >  1:36335/1‑251 (MQ=255)                                                                                                                                                                                     tACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTAtt                                                       <  1:729375/250‑1 (MQ=255)                                                                                                                                                                                     tACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTg                                                      <  1:284076/251‑1 (MQ=255)                                                                                                                                                                                     tACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTg                                                      <  1:155714/251‑1 (MQ=255)                                                                                                                                                                                                 ccATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTc                                          <  1:97568/251‑1 (MQ=255)                                                                                                                                                                                                 ccATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTc                                          <  1:469189/251‑1 (MQ=255)                                                                                                                                                                                                    tcgtcgGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGt                                       <  1:73454/251‑1 (MQ=255)                                                                                                                                                                                                     cgtcgGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATcc                                                                                                                         >  1:58051/1‑168 (MQ=255)                                                                                                                                                                                                          gCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTa                                                                                          >  1:468679/1‑194 (MQ=255)                                                                                                                                                                                                           cACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATcc                                                                                                                         <  1:145045/162‑1 (MQ=255)                                                                                                                                                                                                           cACCAATGCCGGTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATcc                                                                                                                         <  1:152054/162‑1 (MQ=255)                                                                                                                                                                                                                     ggTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCa                       >  1:549228/1‑250 (MQ=255)                                                                                                                                                                                                                      gTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAAttt                                                                                                   <  1:530431/173‑1 (MQ=255)                                                                                                                                                                                                                      gTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTAt                                                        >  1:74364/1‑216 (MQ=255)                                                                                                                                                                                                                      gTGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCAc                      <  1:650491/250‑1 (MQ=255)                                                                                                                                                                                                                       tGAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACt                     <  1:295832/250‑1 (MQ=255)                                                                                                                                                                                                                        gAATTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACttt                   >  1:205902/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTGTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACTTTAt                 <  1:478265/251‑1 (MQ=255)                                                                                                                                                                                                                             gTTAGGCGAAATTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAAcc                                  >  1:722763/1‑231 (MQ=255)                                                                                                                                                                                                                                     aaaTTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGtat                                               <  1:689536/210‑1 (MQ=255)                                                                                                                                                                                                                                     aaaTTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTc                                          >  1:302576/1‑215 (MQ=255)                                                                                                                                                                                                                                     aaaTTGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACTTTATCAAACAAATaa      >  1:31992/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGGTCTTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGTTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACTTTATCAAACAAATAAAGTg  <  1:396080/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       CGTCAATCGCTTACACCGAGCCGGAAGTAGCGTGGGTAGGTAAAACCGAGAAAGAATGTAAAGCCGAAGGCGTAAACTACGAAATCGCAAACTTCCCATGGGCAGCTTCCGGTCGTGCAATCGCTTCCGACTGCGCCGACGGTATGACCAAACTGATTTTCGATAAAGACACGCATCGCGTACTTGGCGGCGCCATCGTCGGCACCAATGCCGGTGAATTGTTAGGCGAAATTGGTATTGCCATCGAAATGGGTTGTGACGCGGAAGACATCGCATTGACTATCCACGCCCACCCGACTTTACACGAATCTGTCGGTCTTGCGGCCGAAGTGTTTGAGGGGTCGGTCACCGACTTACCTAATCCAAAAGCGAAGAAAAAATAATTTGCTTCATTAAAAATAAAAGCCTTTCCGTTGGGAAGGCTTTTATTGTAAGTATAATTCAGTTAACCGATGAAAATCACTTTATCAAACAAATAAAGTG  >  1/976610‑977092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑