Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	971,258	T→C	G47G (GGT→GGC)	unknown →	Pyruvate dehydrogenase E1 component (EC 1.2.4.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	971,258	0	T	C	100.0%	204.1 / NA	73	G47G (GGT→GGC)	unknown	Pyruvate dehydrogenase E1 component (EC 1.2.4.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/27);  total (46/27)

GATTAAACATATTTTAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGTGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGTGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAGTTTGTTTTAATCATTTCTTCAAAGCCG  >  1/971026‑971499                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  gATTAAACATATTTTAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACa                                                                                                                                                                                                                                 >  1:156557/1‑251 (MQ=255) gATTAAACATATTTTAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATCGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACa                                                                                                                                                                                                                                 >  1:37126/1‑251 (MQ=255)          tatTTTAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATta                                                                                                                                                                                                                          >  1:479286/1‑249 (MQ=255)              ttAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAg                                                                                                                                                                                                                    <  1:599485/251‑1 (MQ=255)              ttAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAg                                                                                                                                                                                                                    <  1:756715/251‑1 (MQ=255)                          tCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATa                                                                                                                                                                                                        >  1:838102/1‑251 (MQ=255)                               gttTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGAt                                                                                                                                                                                                    >  1:200385/1‑250 (MQ=255)                               gttTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGAt                                                                                                                                                                                                    >  1:722019/1‑250 (MQ=255)                               gttTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGAt                                                                                                                                                                                                    >  1:626854/1‑250 (MQ=255)                                        cTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAGGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAg                                                                                                                                                                                                                    >  1:438697/1‑225 (MQ=255)                                        cTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGttt                                                                                                                                                                                            >  1:315970/1‑249 (MQ=255)                                             aaaGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAg                                                                                                                                                                                                                    >  1:431504/1‑220 (MQ=255)                                               aGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGAAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAg                                                                                                                                                                                    >  1:48223/1‑250 (MQ=255)                                                    gTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAAc                                                                                                                                                                                      >  1:140505/1‑243 (MQ=255)                                                         aCGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATAc                                                                                                                                                                                                       <  1:248798/221‑1 (MQ=255)                                                         aCGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATc                                                                                                                                                                          >  1:32087/1‑250 (MQ=255)                                                             aCTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTg                                                                                                                                                                                                            <  1:719212/212‑1 (MQ=255)                                                             aCTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGa                                                                                                                                                                     >  1:218079/1‑251 (MQ=255)                                                              cTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATtactac                                                                                                                                                                                                                      <  1:883489/201‑1 (MQ=255)                                                              cTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTg                                                                                                                                                                                                            <  1:373740/211‑1 (MQ=255)                                                              cTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAAc                                                                                                                                                                                      <  1:234870/233‑1 (MQ=255)                                                              cTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGaa                                                                                                                                                                    >  1:579251/1‑251 (MQ=255)                                                              cTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGaa                                                                                                                                                                    >  1:189823/1‑251 (MQ=255)                                                                             aGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTg                                                                                                                                                                                                            >  1:798481/1‑196 (MQ=255)                                                                              gATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGAtt                                                                                                                                                                                                                 >  1:515712/1‑190 (MQ=255)                                                                                   ggAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTa                                                                                                                                               >  1:480404/1‑251 (MQ=255)                                                                                          gCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATtactac                                                                                                                                                                                                                      >  1:862052/1‑173 (MQ=255)                                                                                             aTGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAg                                                                                                                                                                                                                    >  1:414423/1‑172 (MQ=255)                                                                                                    aaTTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCCGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTc                                                                                                                                 <  1:379861/248‑1 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTTGAGAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCtg                                                                                                                    <  1:592162/248‑1 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTg                                                                                                                                                                                         >  1:457785/1‑177 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTc                                                                                                                                 >  1:176303/1‑233 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGc                                                                                                                      >  1:88146/1‑244 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGtgatg                                                                                                               >  1:326979/1‑251 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGtgatg                                                                                                               >  1:501085/1‑251 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGtgatg                                                                                                               >  1:486425/1‑251 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGtgatg                                                                                                               >  1:277525/1‑251 (MQ=255)                                                                                                                   aCGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGtgatg                                                                                                               >  1:85979/1‑251 (MQ=255)                                                                                                                    cGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATc                                                                                                                                                                                                   >  1:487364/1‑166 (MQ=255)                                                                                                                        gATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATAc                                                                                                                                                                                                       <  1:87504/158‑1 (MQ=255)                                                                                                                          tCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGgtgt                                                                                                        <  1:807548/251‑1 (MQ=255)                                                                                                                             gATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTAGTGGCGGCGTTGCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATcc                                                                                                                                                                                                  <  1:770099/158‑1 (MQ=255)                                                                                                                                  aaaCTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCtg                                                                                                                    <  1:274639/231‑1 (MQ=255)                                                                                                                                   aaCTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGt                                                                                                >  1:678029/1‑250 (MQ=255)                                                                                                                                                  ggCAGCAATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTg                                                                                >  1:419474/1‑251 (MQ=255)                                                                                                                                                       caATTGATTCGTTAATTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGAt                                                                              <  1:287338/248‑1 (MQ=255)                                                                                                                                                                    aaTTCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTAt                                                              <  1:722887/251‑1 (MQ=255)                                                                                                                                                                      ttCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGAc                                                                                                                                                            >  1:607771/1‑155 (MQ=255)                                                                                                                                                                      ttCGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCt                                                            <  1:363004/251‑1 (MQ=255)                                                                                                                                                                        cGTGAAGAAGGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTAc                                                          <  1:341270/251‑1 (MQ=255)                                                                                                                                                                             agaagGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGAc                                                                                    <  1:211394/220‑1 (MQ=255)                                                                                                                                                                             agaagGCGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTc                                                      >  1:418839/1‑250 (MQ=255)                                                                                                                                                                                 ggcgtT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGaaaa                                                                                         <  1:753687/208‑1 (MQ=255)                                                                                                                                                                                 ggcgtT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGAc                                                                                    <  1:833032/213‑1 (MQ=255)                                                                                                                                                                                  gcgtT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAAt                                                  <  1:701427/247‑1 (MQ=255)                                                                                                                                                                                  gcgtT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAAt                                                  >  1:577145/3‑249 (MQ=255)                                                                                                                                                                                  gcgtT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAAt                                                  >  1:364066/3‑249 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATg                                                                                                                                   >  1:749425/1‑152 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTAc                                                          <  1:350028/225‑1 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCaa                                         >  1:485353/1‑242 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCaa                                         <  1:341397/242‑1 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATg                                >  1:721382/1‑251 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATg                                >  1:365832/1‑251 (MQ=255)                                                                                                                                                                                                    gTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATg                                >  1:847816/1‑251 (MQ=255)                                                                                                                                                                                                             tGATCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTAc                                                          <  1:826/216‑1 (MQ=255)                                                                                                                                                                                                               aTCAAGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTTCAACGGTTTATGAAg                             <  1:888728/243‑1 (MQ=255)                                                                                                                                                                                                                  aaGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGAtt                                                                             <  1:130628/192‑1 (MQ=255)                                                                                                                                                                                                                   aGTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAg                             >  1:475202/1‑239 (MQ=255)                                                                                                                                                                                                                    gTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGAc                                                                                    >  1:272645/1‑183 (MQ=255)                                                                                                                                                                                                                    gTGATGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAGTTTGTTTTAATCa                >  1:833618/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGCAGCAAGCTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTAc                                                          >  1:21827/1‑206 (MQ=255)                                                                                                                                                                                                                                 cTCGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAGTTTGTTTTAATCATTTCTTCAAAGc    <  1:302023/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGTATTGGCGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAGTTTGTTTTAATCATTTCTTCAAAGCCg  >  1:706013/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  GATTAAACATATTTTAGTTATCAATTCGTTGTTTAATTTCTACAAAAGTAGGTTAAACGTACTTCTAACTTAACAAAGATAAGGAAAAAGCTATGTCAGAATTGTTAAAAAATGACGTAGATCCGATCGAAACTAACGATTGGTTGGCAGCAATTGATTCGTTAATTCGTGAAGAAGGTGTT‑‑GAGCGTGCGCAGTTCATTATTGATCAAGTGATGCAGCAAGCTCGTATTGGTGGCGTTTCTTTGCCGACAGGGATTACTACAGATTACGTGAATACGATCCCGGTTTCTGAACAGCCCGAGTATCCGGGAAATTTAGACATTGAGCGTCGTATTCGTTCTTATGTCCGCTGGAATGCTGTGATGATGGTGTTGCGCGGTCAGAAAAAAGACCTTGATTTAGGCGGTCACTTATCTACTTTCCAATCTGCTGCAACGGTTTATGAAGTTTGTTTTAATCATTTCTTCAAAGCCG  >  1/971026‑971499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑