Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	963,414	A→G	H304H (CAT→CAC)	unknown ←	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	963,414	0	A	G	100.0%	202.9 / NA	74	H304H (CAT→CAC)	unknown	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/30);  total (44/30)

GTGACGCCTACCGCCAAGTCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCATGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAA  >  1/963181‑963659                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      gTGACGCCTACCGCCAAGTCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAg                                                                                                                                                                                                                                       <  1:159685/250‑1 (MQ=255)          aCCGCCAAGTCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCa                                                                                                                                                                                                                              >  1:414954/1‑250 (MQ=255)                  gTCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTaaa                                                                                                                                                                                                                      >  1:193462/1‑250 (MQ=255)                   tCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAAc                                                                                                                                                                                                                     <  1:407463/250‑1 (MQ=255)                   tCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAAc                                                                                                                                                                                                                     <  1:797487/250‑1 (MQ=255)                      cgGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACttt                                                                                                                                                                                                                  <  1:169105/250‑1 (MQ=255)                        gTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCg                                                                                                                                                                                                                >  1:888631/1‑250 (MQ=255)                              gCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGt                                                                                                                                                                                                         >  1:689132/1‑251 (MQ=255)                                cGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTg                                                                                                                                                                                                       <  1:542835/251‑1 (MQ=255)                                       cATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGa                                                                                                                                                                                                >  1:99344/1‑251 (MQ=255)                                       cATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGa                                                                                                                                                                                                >  1:761793/1‑251 (MQ=255)                                                 gcTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCaaa                                                                                                                                                                                      >  1:735271/1‑251 (MQ=255)                                                    ttACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAg                                                                                                                                                                                    >  1:466419/1‑250 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCAcc                                                                                                                                                                                                                            >  1:208763/1‑208 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCAcc                                                                                                                                                                                                                            >  1:483725/1‑208 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAAc                                                                                                                                                                                                                     >  1:100491/1‑215 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACt                                                                                                                                                                                                                    >  1:557357/1‑216 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACt                                                                                                                                                                                                                    >  1:322447/1‑216 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCtt                                                                                                                                                                                 >  1:339715/1‑251 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCtt                                                                                                                                                                                 >  1:873120/1‑251 (MQ=255)                                                      aCATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCCTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCtt                                                                                                                                                                                 >  1:320344/1‑251 (MQ=255)                                                                  gCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTg                                                                                                                                                                     >  1:816854/1‑251 (MQ=255)                                                                       cGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTc                                                                                                                                                                <  1:305225/251‑1 (MQ=255)                                                                             gttgttGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGAttt                                                                                                                                                          >  1:764573/1‑251 (MQ=255)                                                                                             gAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAg                                                                                                                                                                                                                                       >  1:272858/1‑158 (MQ=255)                                                                                             gAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTaaa                                                                                                                                                                                                                      >  1:829686/1‑175 (MQ=255)                                                                                             gAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCa                                                                                                                                                                                                   <  1:113130/194‑1 (MQ=255)                                                                                             gAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCggcaa                                                                                                                                          >  1:397727/1‑251 (MQ=255)                                                                                               aTTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAg                                                                                                                                                                                                                                       >  1:842328/1‑156 (MQ=255)                                                                                               aTTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCggcaaag                                                                                                                                        >  1:263501/1‑251 (MQ=255)                                                                                                     aCCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCaaaa                                                                                                                                  <  1:119538/251‑1 (MQ=255)                                                                                                          gCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGc                                                                                                                               <  1:133365/249‑1 (MQ=255)                                                                                                          gCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGc                                                                                                                               <  1:814323/249‑1 (MQ=255)                                                                                                                    cGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAAt                                                                                                                   <  1:637868/251‑1 (MQ=255)                                                                                                                       tttCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAAttt                                                                                                                 >  1:82571/1‑250 (MQ=255)                                                                                                                        ttCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCa                                                                                                               >  1:499564/1‑251 (MQ=255)                                                                                                                         tCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAAt                                                                                                                                                 >  1:721381/1‑216 (MQ=255)                                                                                                                           cTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACg                                                                                                            <  1:126574/251‑1 (MQ=255)                                                                                                                            tCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACg                                                                                                            >  1:593651/1‑250 (MQ=255)                                                                                                                               ccATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGcc                                                                                                                                                                                                              >  1:884284/1‑149 (MQ=255)                                                                                                                               ccATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGc                                                                                                                                                                    <  1:419797/191‑1 (MQ=255)                                                                                                                               ccATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCAt                                                                                                        >  1:756029/1‑251 (MQ=255)                                                                                                                               ccATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCAt                                                                                                        >  1:672176/1‑251 (MQ=255)                                                                                                                               ccATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGAAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCAt                                                                                                        >  1:664868/1‑251 (MQ=255)                                                                                                                                    ttGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCt                                                                                                   <  1:457419/251‑1 (MQ=255)                                                                                                                                      gACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCg                                                                                                 >  1:665059/1‑251 (MQ=255)                                                                                                                                           cggAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGt                                                                                             <  1:166125/250‑1 (MQ=255)                                                                                                                                           cggAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGt                                                                                             <  1:559080/250‑1 (MQ=255)                                                                                                                                             gAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTaat                                                                                          >  1:355938/1‑251 (MQ=255)                                                                                                                                              aaTTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTaatc                                                                                         <  1:4490/251‑1 (MQ=255)                                                                                                                                                           ccTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTg                                                                                                                                                                     <  1:826838/162‑1 (MQ=255)                                                                                                                                                           ccTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGc                                                                                                                                                                    <  1:294641/163‑1 (MQ=255)                                                                                                                                                            cTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTcggcgg                                                                           <  1:809473/251‑1 (MQ=255)                                                                                                                                                                gAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTaatc                                                                                         <  1:351936/233‑1 (MQ=255)                                                                                                                                                                                           cAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCGTCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACtt                                             >  1:460242/1‑250 (MQ=255)                                                                                                                                                                                                 tCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGa                                      >  1:833896/1‑251 (MQ=255)                                                                                                                                                                                                  cccAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGaa                                     >  1:270392/1‑251 (MQ=255)                                                                                                                                                                                                      aCCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTaa                                 >  1:465490/1‑251 (MQ=255)                                                                                                                                                                                                       ccccATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAg                                                                                                                                                                                                                                       <  1:149030/52‑1 (MQ=255)                                                                                                                                                                                                        cccATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAAc                               <  1:640142/251‑1 (MQ=255)                                                                                                                                                                                                        cccATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAAc                               <  1:881894/251‑1 (MQ=255)                                                                                                                                                                                                         ccATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAAcc                              <  1:645475/251‑1 (MQ=255)                                                                                                                                                                                                         ccATTGCTCGCGATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAAcc                              <  1:423056/251‑1 (MQ=255)                                                                                                                                                                                                                  gcgATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGc                                                                                                                               >  1:754717/1‑145 (MQ=255)                                                                                                                                                                                                                   cgATATCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTgcgc                    <  1:554662/251‑1 (MQ=255)                                                                                                                                                                                                                     atatCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTCAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTgcgc                    >  1:787356/1‑249 (MQ=255)                                                                                                                                                                                                                     atatCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTgcgc                    >  1:88901/1‑249 (MQ=255)                                                                                                                                                                                                                     atatCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTgcgc                    >  1:822725/1‑249 (MQ=255)                                                                                                                                                                                                                     atatCGGGCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGcc                   <  1:631256/250‑1 (MQ=255)                                                                                                                                                                                                                           ggCGCTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTgcg             >  1:618918/1‑250 (MQ=255)                                                                                                                                                                                                                            gcgcTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTgcgc            <  1:648419/250‑1 (MQ=255)                                                                                                                                                                                                                              gcTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCttt         >  1:358442/1‑251 (MQ=255)                                                                                                                                                                                                                               cTTAAATGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTttg        >  1:767699/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGGGCGTGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGaaa  <  1:12479/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      GTGACGCCTACCGCCAAGTCGCGGTACATGCCGATGGTCATTTGTTGCGCTTTACATAAGGCATCGCATTCGGCAAGTTGTTGGTCGGCGCAGAATTGCAACCAGGCATAAAATTCGATTTCCTCACCATATTGACGGCGGAATTGCGCGACGACCTCGGAATGATAATCTTGGAATTCCGGCGCCCAGAAATCCCAACCCCATTGCTCGCGATATCGGGCGCTTAAATGGGCATGCAGCGCATCAAAAGTGGCTTGCACCTGTAAACTTTCGCCACCGTTGCGCACGAAATCGGCAAAAGCTTGTTGGGAAAGTGCGGTCGGATTTTCTTTAAATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAA  >  1/963181‑963659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑