Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	962,927	A→G	S467P (TCG→CCG)	unknown ←	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	962,927	0	A	G	100.0%	207.0 / NA	74	S467P (TCG→CCG)	unknown	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/40);  total (34/40)

TGCTTAGGGTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGAGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTCCTTATCGCTCCAGGTTT  >  1/962684‑963166                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                tGCTTAGGGTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCg                                                                                                                                                                                                                                          >  1:699478/1‑251 (MQ=255)  gCTTAGGGTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCg                                                                                                                                                                                                                                          >  1:583196/1‑250 (MQ=255)       gggTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCttt                                                                                                                                                                                                                                     >  1:310503/1‑250 (MQ=255)       gggTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAAGGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCttt                                                                                                                                                                                                                                     >  1:127412/1‑250 (MQ=255)            gtCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGATGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGAt                                                                                                                                                                                                                               >  1:66367/1‑251 (MQ=255)                tCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATccac                                                                                                                                                                                                                           <  1:224492/251‑1 (MQ=255)                       gTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATaaa                                                                                                                                                                                                                     >  1:879974/1‑250 (MQ=255)                       gTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATaaa                                                                                                                                                                                                                     >  1:700839/1‑250 (MQ=255)                       gTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATaaa                                                                                                                                                                                                                     >  1:840437/1‑250 (MQ=255)                        tACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCAGTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACg                                                                                                                                                                                                                   <  1:430956/251‑1 (MQ=255)                           ggATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCaa                                                                                                                                                                                                                >  1:297946/1‑251 (MQ=255)                                 tCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACg                                                                                                                                                                                                           <  1:617159/250‑1 (MQ=255)                                    cGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGTTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACAt                                                                                                                                                                                                       <  1:441933/251‑1 (MQ=255)                                        ggTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAAtg                                                                                                                                                                                                   >  1:490393/1‑251 (MQ=255)                                        ggTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAAtg                                                                                                                                                                                                   >  1:60103/1‑251 (MQ=255)                                        ggTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAAtg                                                                                                                                                                                                   >  1:36092/1‑251 (MQ=255)                                         gTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCGCGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGAt                                                                                                                                                                                                                               <  1:867197/222‑1 (MQ=255)                                         gTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATcc                                                                                                                                                                                                                             <  1:498160/224‑1 (MQ=255)                                         gTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAAtg                                                                                                                                                                                                   <  1:187744/250‑1 (MQ=255)                                               aCTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGATGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAAAGACATAATGTGATCgg                                                                                                                                                                                            >  1:867811/1‑250 (MQ=255)                                               aCTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATccacc                                                                                                                                                                                                                          >  1:350408/1‑221 (MQ=255)                                                     tCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGc                                                                                                                                                                                       <  1:563107/250‑1 (MQ=255)                                                       cTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAAt                                                                                                                                                                                    <  1:767272/251‑1 (MQ=255)                                                       cTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAAt                                                                                                                                                                                    <  1:477342/251‑1 (MQ=255)                                                        ttGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGAt                                                                                                                                                                                           <  1:229177/243‑1 (MQ=255)                                                         tGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGc                                                                                                                                                                                  <  1:510481/251‑1 (MQ=255)                                                           tttATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTGTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCAc                                                                                                                                                                                <  1:690518/251‑1 (MQ=255)                                                              aTCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGc                                                                                                                                                                             <  1:745835/251‑1 (MQ=255)                                                              aTCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGc                                                                                                                                                                             <  1:564415/251‑1 (MQ=255)                                                                         aaTAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCata                                                                                                                                                                  <  1:398013/251‑1 (MQ=255)                                                                           tAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATAtt                                                                                                                                                                <  1:506047/251‑1 (MQ=255)                                                                                    ttGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCaa                                                                                                                                                                           <  1:562681/231‑1 (MQ=255)                                                                                    ttGTAAGACAAAATGCCGGCATTTTTGAGTTTGCGGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCa                                                                                                                                                        >  1:705134/1‑250 (MQ=255)                                                                                            cAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGc                                                                                                                                                                                       >  1:818645/1‑211 (MQ=255)                                                                                              aaaTGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATa                                                                                                                                             <  1:221260/251‑1 (MQ=255)                                                                                                         tttttGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATa                                                                                                                                  >  1:384686/1‑251 (MQ=255)                                                                                                         aaactcaGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATa                                                                                                                                  <  1:390851/245‑1 (MQ=255)                                                                                                           tttGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAg                                                                                                                                <  1:425389/251‑1 (MQ=255)                                                                                                           tttGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAg                                                                                                                                <  1:712168/251‑1 (MQ=255)                                                                                                               aGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAgcgc                                                                                                                             <  1:575349/250‑1 (MQ=255)                                                                                                                           aTTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGaa                                                                                                                 <  1:830600/250‑1 (MQ=255)                                                                                                                               ctttcGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGCGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGaa                                                                                                                 >  1:195572/1‑246 (MQ=255)                                                                                                                                      cACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTc                                                                                                      <  1:727556/250‑1 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCggg                                                                                                                                                                                                                                 <  1:572859/123‑1 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAg                                                                                                                                >  1:793965/1‑220 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGtt                                                                                                       >  1:823297/1‑245 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCgg                                                                                                 >  1:584511/1‑251 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCgg                                                                                                 >  1:838777/1‑251 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCgg                                                                                                 >  1:816104/1‑251 (MQ=255)                                                                                                                                          gTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCgg                                                                                                 >  1:1274/1‑251 (MQ=255)                                                                                                                                            gCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTc                                                                                                                                                 >  1:897014/1‑201 (MQ=255)                                                                                                                                                  aTCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGc                                                                                         <  1:83801/251‑1 (MQ=255)                                                                                                                                                  aTCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGc                                                                                         <  1:538770/251‑1 (MQ=255)                                                                                                                                                              aaTCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTc                                                                             <  1:606394/251‑1 (MQ=255)                                                                                                                                                                tCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTc                                                                                                                                                 <  1:723183/181‑1 (MQ=255)                                                                                                                                                                tCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCt                                                                           <  1:544816/251‑1 (MQ=255)                                                                                                                                                                   cGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTg                                                                         >  1:877839/1‑250 (MQ=255)                                                                                                                                                                   cGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTg                                                                         >  1:418259/1‑250 (MQ=255)                                                                                                                                                                       gCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCatat                                                                                                                                                                 <  1:450232/158‑1 (MQ=255)                                                                                                                                                                             acgTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAaca                                                              <  1:109206/251‑1 (MQ=255)                                                                                                                                                                                 tGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATc                                                          <  1:641860/251‑1 (MQ=255)                                                                                                                                                                                 tGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATc                                                          <  1:686132/251‑1 (MQ=255)                                                                                                                                                                                  ggCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATc                                                          >  1:565207/1‑250 (MQ=255)                                                                                                                                                                                                  aaTGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACgg                                         <  1:629134/251‑1 (MQ=255)                                                                                                                                                                                                              atcatcGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGa                              >  1:375296/1‑250 (MQ=255)                                                                                                                                                                                                              atcatcGACCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGAc                             <  1:409264/251‑1 (MQ=255)                                                                                                                                                                                                                     aCCGGATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACaa                      >  1:225652/1‑251 (MQ=255)                                                                                                                                                                                                                        ggATAACGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAAt                     >  1:173816/1‑249 (MQ=255)                                                                                                                                                                                                                              cGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAacac                                                             <  1:685590/203‑1 (MQ=255)                                                                                                                                                                                                                              cGCACATAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGAc                             <  1:780962/235‑1 (MQ=255)                                                                                                                                                                                                                                  caTAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTc                   >  1:43438/1‑241 (MQ=255)                                                                                                                                                                                                                                  caTAGGCGCCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTCCTTATCGCt          >  1:575421/1‑250 (MQ=255)                                                                                                                                                                                                                                        cgcCGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTCCTTATCGCTCCAGGtt   <  1:496674/251‑1 (MQ=255)                                                                                                                                                                                                                                          ccGTTGACCGGGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTCCTTATCGCTCCAGGttt  >  1:540417/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                TGCTTAGGGTGGTCATCGCTTGGTACGGATAGTCCCGCAGGTCACGACTTTGTCCTTGTTTATCAAATTCAAAATAGAAGATTTTGTAAGACAAAATGCCGGCATTTTTGAGTTTGCTGACGATTTCTTTCGGCACGGTGCCTAAATCTTCGCCGATAATCACGCAGCGACGACGTTGGCTTTCAAGGGCGAGAATGGCGATTAAATCATCGACCGGATAACGCACATAGGCGCCGTTGACCGAGCTGTCGCCTTTCGGGATCCACCATAAACGCAATAACGACATAATGTGATCGATACGCAATGCACCGCAATGATTCATATTGGCGCGCACCAAGTCGATAAACGGCTGATAAGCGCGTTGTTTCAGAATGTGCGGGTTCATCGGCGTTAAGCCCCAGTTTTGTCCTTGCGGACCTAACACATCCGGCGGCGCACCCACGGACGCCCGCAGACAGTACAATTCCTTATCGCTCCAGGTTT  >  1/962684‑963166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑