Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	956,189	C→T	L68F (CTC→TTC)	unknown →	2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	956,189	0	C	T	100.0%	277.7 / NA	97	L68F (CTC→TTC)	unknown	2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/57);  total (40/57)

GTCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCCTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGTGAGCGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGTTGGCGGAAATCGCACCTTCGTTTTCCTTGCCGAACGGGCAATCCATCAGGGAG  >  1/955943‑956422                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gTCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCGAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCg                                                                                                                                                                                                                                         >  1:448110/1‑250 (MQ=255) gTCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCg                                                                                                                                                                                                                                         >  1:685174/1‑250 (MQ=255) gTCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCg                                                                                                                                                                                                                                         >  1:479173/1‑250 (MQ=255)  tCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCa                                                                                                                                                                                                                                       <  1:680027/251‑1 (MQ=255)              cTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTtgatga                                                                                                                                                                                                                            <  1:846872/250‑1 (MQ=255)                   cTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTAc                                                                                                                                                                                                                       <  1:217537/250‑1 (MQ=255)                    tACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACaa                                                                                                                                                                                                                     <  1:862283/251‑1 (MQ=255)                    tACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACCGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACaa                                                                                                                                                                                                                     <  1:426708/251‑1 (MQ=255)                              acGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAAc                                                                                                                                                                                                           <  1:421898/251‑1 (MQ=255)                                gAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAg                                                                                                                                                                                                         >  1:410721/1‑251 (MQ=255)                                  aTAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGa                                                                                                                                                                                                       <  1:754226/251‑1 (MQ=255)                                   tAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGaa                                                                                                                                                                                                      <  1:373064/251‑1 (MQ=255)                                   tAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGaa                                                                                                                                                                                                      <  1:596998/251‑1 (MQ=255)                                   tAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGaa                                                                                                                                                                                                      <  1:803473/251‑1 (MQ=255)                                    aaGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAAc                                                                                                                                                                                                     <  1:164433/251‑1 (MQ=255)                                         tGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                <  1:315924/251‑1 (MQ=255)                                          gCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                >  1:651646/1‑250 (MQ=255)                                          gCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                >  1:299698/1‑250 (MQ=255)                                          gCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                >  1:384810/1‑250 (MQ=255)                                           cccATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAAc                                                                                                                                                                                                           >  1:388744/1‑238 (MQ=255)                                             cATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAATAGGAACAAGGGCgtg                                                                                                                                                                                            >  1:870753/1‑251 (MQ=255)                                               tGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTAt                                                                                                                                                                                                                >  1:51923/1‑229 (MQ=255)                                               tGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                <  1:376838/245‑1 (MQ=255)                                               tGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCgtgt                                                                                                                                                                                           >  1:48202/1‑250 (MQ=255)                                               tGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCgtgt                                                                                                                                                                                           >  1:559614/1‑250 (MQ=255)                                               tGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTc                                                                                                                                                                                          <  1:67711/251‑1 (MQ=255)                                                gAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTc                                                                                                                                                                                          >  1:899660/1‑250 (MQ=255)                                                         gtATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAgg                                                                                                                                                                                                        <  1:154861/227‑1 (MQ=255)                                                         gtATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                >  1:550395/1‑235 (MQ=255)                                                         gtATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTtcgt                                                                                                                                                                                 >  1:183424/1‑250 (MQ=255)                                                              tCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTtgatga                                                                                                                                                                                                                            <  1:249888/202‑1 (MQ=255)                                                                gCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAAc                                                                                                                                                                                                           <  1:480276/217‑1 (MQ=255)                                                                          gTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGcaca                                                                                                                                                               >  1:392783/1‑251 (MQ=255)                                                                          gTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGcaca                                                                                                                                                               >  1:891002/1‑251 (MQ=255)                                                                           tAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGcac                                                                                                                                                                <  1:504534/249‑1 (MQ=255)                                                                           tAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACAt                                                                                                                                                              >  1:586595/1‑251 (MQ=255)                                                                                tAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACA‑GAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑c                                                                                                                                                       <  1:780263/251‑1 (MQ=255)                                                                                   cGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑c                                                                                                                                                       <  1:552776/249‑1 (MQ=255)                                                                                   cGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑c                                                                                                                                                       <  1:727440/249‑1 (MQ=255)                                                                                     gATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGa                                                                                                                                                   >  1:577808/1‑251 (MQ=255)                                                                                      aTCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGat                                                                                                                                                  <  1:36315/251‑1 (MQ=255)                                                                                        ccGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGatat                                                                                                                                                <  1:30493/251‑1 (MQ=255)                                                                                            aaGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTg                                                                                                                                            >  1:344731/1‑251 (MQ=255)                                                                                             aGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTg                                                                                                                                            >  1:560436/1‑250 (MQ=255)                                                                                              gCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATAtttt                                                                                                                                             <  1:371573/248‑1 (MQ=255)                                                                                                   aCTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAAc                                                                                                                                                                                                           <  1:61542/182‑1 (MQ=255)                                                                                                    cTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTTTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGc                                                                                                                                    <  1:439630/251‑1 (MQ=255)                                                                                                           ccgcATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCaa                                                                                                                             >  1:898100/1‑251 (MQ=255)                                                                                                              cATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGATCCTGGATATTTTGCTTTACGCCGATCaaa                                                                                                                            >  1:899941/1‑250 (MQ=255)                                                                                                              cATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTg                                                                                                                                            >  1:177841/1‑233 (MQ=255)                                                                                                                  aCGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTc                                                                                                                      <  1:105047/251‑1 (MQ=255)                                                                                                                         aTGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑c                                                                                                                                                       <  1:615001/211‑1 (MQ=255)                                                                                                                           gAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGaa                                                                                                              >  1:808330/1‑250 (MQ=255)                                                                                                                              tCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTACGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTc                                                                                                          <  1:449587/251‑1 (MQ=255)                                                                                                                                   tGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAGACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACt                                                                                                     <  1:814354/251‑1 (MQ=255)                                                                                                                                   tGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACt                                                                                                     <  1:128811/251‑1 (MQ=255)                                                                                                                                             ccGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCAtt                                                                                           <  1:295788/251‑1 (MQ=255)                                                                                                                                             ccGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCAt                                                                                            <  1:700200/250‑1 (MQ=255)                                                                                                                                             ccGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCAt                                                                                            <  1:706287/250‑1 (MQ=255)                                                                                                                                              cGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                <  1:176539/150‑1 (MQ=255)                                                                                                                                                 cTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGa                                                                                       <  1:819224/251‑1 (MQ=255)                                                                                                                                                 cTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGa                                                                                       <  1:600719/251‑1 (MQ=255)                                                                                                                                                 cTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGa                                                                                       <  1:893686/251‑1 (MQ=255)                                                                                                                                                   gATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGata                                                                                                                                                 <  1:84927/191‑1 (MQ=255)                                                                                                                                                   gATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCAt                                                                                            >  1:893092/1‑244 (MQ=255)                                                                                                                                                   gATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGata                                                                                     >  1:295967/1‑251 (MQ=255)                                                                                                                                                   gATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGa                                                                                       >  1:867927/1‑249 (MQ=255)                                                                                                                                                      tAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGc                                                                                  <  1:123696/251‑1 (MQ=255)                                                                                                                                                      tAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGc                                                                                  <  1:787216/251‑1 (MQ=255)                                                                                                                                                        gCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGata                                                                                                                                                 >  1:486014/1‑186 (MQ=255)                                                                                                                                                         cGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGcac                                                                                <  1:603400/250‑1 (MQ=255)                                                                                                                                                         cGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGcac                                                                                <  1:575526/250‑1 (MQ=255)                                                                                                                                                         cGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGcac                                                                                <  1:508576/250‑1 (MQ=255)                                                                                                                                                           tCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAAt                                                                             <  1:99263/251‑1 (MQ=255)                                                                                                                                                                 cTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCg                                                                           <  1:804459/247‑1 (MQ=255)                                                                                                                                                                  tttttATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAAt                                                                      <  1:685807/251‑1 (MQ=255)                                                                                                                                                                  tttttATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAAt                                                                      <  1:236996/251‑1 (MQ=255)                                                                                                                                                                  tttttATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAAt                                                                      <  1:246703/251‑1 (MQ=255)                                                                                                                                                                        tCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTaa                                                                <  1:872301/251‑1 (MQ=255)                                                                                                                                                                           aaGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGcac                                                                                >  1:771825/1‑232 (MQ=255)                                                                                                                                                                            aGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAggg                                                                                                                                                                                                >  1:609215/1‑120 (MQ=255)                                                                                                                                                                                  ccTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGtt                                                      >  1:557423/1‑251 (MQ=255)                                                                                                                                                                                           ccGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAAcc                                                                                                                          <  1:678815/174‑1 (MQ=255)                                                                                                                                                                                                aGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑c                                                                                                                                                       >  1:684617/1‑140 (MQ=255)                                                                                                                                                                                                aGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGAGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATcc                                                          >  1:726229/1‑233 (MQ=255)                                                                                                                                                                                                 gATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGa                                                                                       <  1:847923/203‑1 (MQ=255)                                                                                                                                                                                                      gCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTgggg                                                                                                                                                                         >  1:390194/1‑117 (MQ=255)                                                                                                                                                                                                          gATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTc                                                                                                                      >  1:485615/1‑163 (MQ=255)                                                                                                                                                                                                          gATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCg                                                        >  1:208355/1‑225 (MQ=255)                                                                                                                                                                                                               tGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGTTGGCGGAAATCGCACCTTCGTTTTCCTTg                          >  1:479735/1‑250 (MQ=255)                                                                                                                                                                                                                gTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCAt                                                                                            <  1:82103/183‑1 (MQ=255)                                                                                                                                                                                                                                 ccTGGAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCg                                                                           <  1:469022/183‑1 (MQ=255)                                                                                                                                                                                                                                     gAAACGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGTTGGCGGAAATCGCACCTTCGTTTTCCTTGCCGAACGGGCAATCCATCAggg    <  1:631139/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaaCGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGTTGGCGGAAATCGCACCTTCGTTTTCCTTGCCGAACGGGCAATCCATCAGGGAg  <  1:634028/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGGCGCTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGt                                                                                                                   >  1:648408/1‑135 (MQ=255)                                                                                                                                                                                                                                             gcTTTCTCCCTTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGGGAACGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTc                                                                                                                      >  1:272169/1‑128 (MQ=255)                                                                                                                                                                                                                                                  ctcCCTTCGCATTGCTTGATGAATTACAACGTATTGAAc                                                                                                                                                                                                           <  1:848930/39‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GTCGTAAACGTAGCTCCGCTACGAAAAACACGAATAAGGCTGCCCATGAATAAGGTGTATGTCGCTTTAGGCAGTAATTTAGCGGATCCGCAAGCACAACTGGTCGCCGCATTACGGGCGATGAATCAATTGCCTGGCAGCCGTCTGATTAGCGTCAGTTCTTTTTATCAAAGCAAACCTCTCGGTCCGCAAGATCAGCCTGATTATGTCAACGCCGTCGCTTGCCTGGAAACGGCGCTTTCTCCCCTCGCATTGCTTGATGAATTACAACGTATTGAACAGGAACAAGGGCGTGTCCGTCTTCGTCGTTGGGGTGAGCGCACATTAGAT‑CTGGATATTTTGCTTTACGCCGATCAAACCATTCAGTCGGAACGTCTGACTGTTCCCCATTATGATATGCACAATCGTGAATTTGTAATTATCCCGTTGGCGGAAATCGCACCTTCGTTTTCCTTGCCGAACGGGCAATCCATCAGGGAG  >  1/955943‑956422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑