Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	945,015	A→C	R324S (AGA→AGC)	unknown →	Possible ABC transporter, periplasmic substrate X binding protein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	945,015	0	A	C	100.0%	222.7 / NA	77	R324S (AGA→AGC)	unknown	Possible ABC transporter, periplasmic substrate X binding protein precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (49/28);  total (49/28)

CAGCTTAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGCATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGACAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAAC  >  1/944781‑945261                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       cAGCTTAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGtt                                                                                                                                                                                                                                         >  1:638478/1‑250 (MQ=255)   gCTTAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGt                                                                                                                                                                                                                                      >  1:35853/1‑251 (MQ=255)   gCTTAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGt                                                                                                                                                                                                                                      >  1:4249/1‑251 (MQ=255)      tAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCg                                                                                                                                                                                                                                   >  1:869358/1‑251 (MQ=255)            gCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGAt                                                                                                                                                                                                                             >  1:308531/1‑251 (MQ=255)            gCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGAt                                                                                                                                                                                                                             >  1:790514/1‑251 (MQ=255)            gCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGAACACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGAt                                                                                                                                                                                                                             >  1:157425/1‑251 (MQ=255)             ccTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATa                                                                                                                                                                                                                            >  1:494867/1‑251 (MQ=255)              cTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGAGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTA‑‑‑CCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCa                                                                                                                                                                                                                        <  1:90828/251‑1 (MQ=255)                aaaTGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGt                                                                                                                                                                                                                          >  1:452736/1‑250 (MQ=255)                    gACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAAt                                                                                                                                                                                                                      >  1:330951/1‑250 (MQ=255)                               ggTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAg                                                                                                                                                                                                          >  1:843041/1‑251 (MQ=255)                                      gTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATcc                                                                                                                                                                                                                    <  1:445392/234‑1 (MQ=255)                                      gTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCAt                                                                                                                                                                                                   >  1:812483/1‑251 (MQ=255)                                      gTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCAt                                                                                                                                                                                                   >  1:809245/1‑251 (MQ=255)                                      gTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCAt                                                                                                                                                                                                   >  1:131193/1‑251 (MQ=255)                                       tctcCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCAtt                                                                                                                                                                                                  >  1:223055/1‑251 (MQ=255)                                                     gATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGccc                                                                                                                                                                                     >  1:282079/1‑250 (MQ=255)                                                        cACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATg                                                                                                                                                                                 >  1:760947/1‑251 (MQ=255)                                                        cACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATg                                                                                                                                                                                 >  1:360451/1‑251 (MQ=255)                                                          cTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATgg                                                                                                                                                                                >  1:319507/1‑250 (MQ=255)                                                          cTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGgc                                                                                                                                                                               <  1:500036/251‑1 (MQ=255)                                                                  gCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTttct                                                                                                                                                                                              <  1:64663/228‑1 (MQ=255)                                                                   cTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGc                                                                                                                                                                        <  1:795240/249‑1 (MQ=255)                                                                     tGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATgg                                                                                                                                                                                >  1:308293/1‑239 (MQ=255)                                                                     tGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGaa                                                                                                                                                                    >  1:465228/1‑251 (MQ=255)                                                                        aaaaaCAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATg                                                                                                                                                                 <  1:302540/251‑1 (MQ=255)                                                                                    cAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCAc                                                                                                                                                      >  1:707849/1‑250 (MQ=255)                                                                                                 cTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGAtt                                                                                                                                         <  1:70427/250‑1 (MQ=255)                                                                                                     aaaTTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACg                                                                                                                                    >  1:89426/1‑251 (MQ=255)                                                                                                          cTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAg                                                                                                                               >  1:769953/1‑251 (MQ=255)                                                                                                           tACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTGCACGTTAAgg                                                                                                                              <  1:218878/251‑1 (MQ=255)                                                                                                            aCATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAggg                                                                                                                             <  1:153091/251‑1 (MQ=255)                                                                                                                 ccGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGtt                                                                                                                                                                                                                                         <  1:49916/138‑1 (MQ=255)                                                                                                                    aaaTTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCa                                                                                                                                                                                                                        >  1:40550/1‑152 (MQ=255)                                                                                                                      aTTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAggg                                                                                                                             <  1:210065/241‑1 (MQ=255)                                                                                                                      aTTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAAt                                                                                                                     >  1:269049/1‑249 (MQ=255)                                                                                                                       ttCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCtt                                                                                                                  <  1:133180/251‑1 (MQ=255)                                                                                                                                ccGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACCTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGCTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACCCCATTTTCGTCTCCGCCCCATGGCTCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAat                                                                                                          <  1:455413/250‑1 (MQ=255)                                                                                                                                          gTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAaaga                                                                                                                                                <  1:717840/202‑1 (MQ=255)                                                                                                                                          gTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAgggg                                                                                                                            <  1:167489/222‑1 (MQ=255)                                                                                                                                          gTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCt                                                                                                                   >  1:22384/1‑231 (MQ=255)                                                                                                                                          gTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGtt                                                                                               >  1:218026/1‑251 (MQ=255)                                                                                                                                            aaTGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGtttt                                                                                             >  1:872559/1‑251 (MQ=255)                                                                                                                                               gtgATGACGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCACCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGttttt                                                                                            >  1:249288/1‑249 (MQ=255)                                                                                                                                                tgatgaCGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCt                                                                                          <  1:779971/250‑1 (MQ=255)                                                                                                                                                  atgaCGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTaa                                                                                        <  1:108652/250‑1 (MQ=255)                                                                                                                                                   tgaCGGTGGCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAgg                                                                                      <  1:804488/251‑1 (MQ=255)                                                                                                                                                          ggCGAAAAATACACCGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGc                                                                                 >  1:104139/1‑249 (MQ=255)                                                                                                                                                                       ccGAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAg                                                                  >  1:881461/1‑251 (MQ=255)                                                                                                                                                                         gAACCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGGGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTg                                                                >  1:334816/1‑251 (MQ=255)                                                                                                                                                                           aCCCTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTaa                                                                                        >  1:356776/1‑225 (MQ=255)                                                                                                                                                                              cTGCGGTATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAgg                                                                                      >  1:466890/1‑224 (MQ=255)                                                                                                                                                                                   gtaTCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACaaa                                                      >  1:106553/3‑251 (MQ=255)                                                                                                                                                                                     aTCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTc                                                                                                                                       <  1:869804/168‑1 (MQ=255)                                                                                                                                                                                     aTCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACaaaaa                                                    >  1:747576/1‑251 (MQ=255)                                                                                                                                                                                      tCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCCATCCGGCTTAAAAGACACCAttt                                                                                                                                                                                                 <  1:365652/109‑1 (MQ=255)                                                                                                                                                                                      tCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACaaaaaa                                                   >  1:119415/1‑251 (MQ=255)                                                                                                                                                                                       cTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAgg                                                                                      >  1:747000/1‑215 (MQ=255)                                                                                                                                                                                       cTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACaaaaaa                                                   >  1:405088/1‑250 (MQ=255)                                                                                                                                                                                       cTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACaaaaaa                                                   >  1:218385/1‑250 (MQ=255)                                                                                                                                                                                            gtgtTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGt                                             >  1:527587/1‑251 (MQ=255)                                                                                                                                                                                                  gTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGttttttt                                       >  1:616441/1‑251 (MQ=255)                                                                                                                                                                                                         ggATTACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTg                                >  1:144070/1‑251 (MQ=255)                                                                                                                                                                                                            ttACTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCt                             <  1:875073/251‑1 (MQ=255)                                                                                                                                                                                                               cTTCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTg                          >  1:859467/1‑251 (MQ=255)                                                                                                                                                                                                                 tCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGcc                        <  1:655897/251‑1 (MQ=255)                                                                                                                                                                                                                 tCCCCAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGcc                        <  1:371808/251‑1 (MQ=255)                                                                                                                                                                                                                  ccccAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCa                       >  1:445481/1‑251 (MQ=255)                                                                                                                                                                                                                  ccccAGAAGTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCa                       >  1:609463/1‑251 (MQ=255)                                                                                                                                                                                                                          gTGCAACAAAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCt                                                                               >  1:214126/1‑187 (MQ=255)                                                                                                                                                                                                                               acaaAAATTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGaaa                                                                                                                                                                   <  1:432639/98‑1 (MQ=255)                                                                                                                                                                                                                                  aaaaTTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGCCCCGTTAgggg       <  1:872403/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaaTTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGCCCCGTTAgggg       <  1:378818/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCgg                                                             >  1:887932/1‑196 (MQ=255)                                                                                                                                                                                                                                    aaTTAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGc                  <  1:92519/238‑1 (MQ=255)                                                                                                                                                                                                                                       tAAGCCAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAAc  <  1:14481/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CAGCTTAACCCGCCTAAATGACGGCGAATTGGTGATTGTCTCCGCCTGGCAGGATCACTTGTTCAGCTTGCAAAAACAAGGCGCAATCACCGACCGCTTAAAATTCTACATTCCGAAATTCGGCATGCCGGGCGGCGGTAATGTGATGACGGTGGCGAAAAATACACCGAACCCTGCGGCATCTTTAGTGTTTGTCCACTGGATTACTTCCCCAGAAGTGCAACAAAAATTAAGACAAGAATTTGGGGTTCGTCCGTTAGATAGTCAATCCGGCTTAAAAGACACCATTTTCTTCTCCGCCCCATGGCGCAAAGCTGAAATGGAATCTTTCACCAAAGAAGTGATTTCACGTTAAGGGGAGCGAATCTTATTAAAATATCGGCACGTTTTTCTAAGGCGTGCCTTTCATCCAAAAAGTGCGGTCACAAAAAACGACGTTTTTTTAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAAC  >  1/944781‑945261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑