Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	932,812	G→T	I162I (ATC→ATA)	unknown ←	Tyrosyl‑tRNA synthetase (EC 6.1.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	932,812	0	G	T	100.0%	198.5 / NA	70	I162I (ATC→ATA)	unknown	Tyrosyl‑tRNA synthetase (EC 6.1.1.1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/34);  total (36/34)

TTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATCTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCGATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGGGTGGTGTTTTTACCGGACG  >  1/932568‑933053                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                                                                                                             <  1:282850/251‑1 (MQ=255) tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                                                                                                             <  1:283770/251‑1 (MQ=255) tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                                                                                                             <  1:430431/251‑1 (MQ=255)      cTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggttggtt                                                                                                                                                                                                                                        <  1:653849/251‑1 (MQ=255)          gTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTAttt                                                                                                                                                                                                                                    >  1:388845/1‑251 (MQ=255)          gTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTAttt                                                                                                                                                                                                                                    >  1:796117/1‑251 (MQ=255)           tGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTg                                                                                                                                                                                                                                   <  1:866425/251‑1 (MQ=255)                        ccGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGtttttt                                                                                                                                                                                                                      >  1:847647/1‑251 (MQ=255)                           aTGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGaa                                                                                                                                                                                                                   >  1:50078/1‑251 (MQ=255)                             gTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAat                                                                                                                                                                                                                 >  1:529145/1‑251 (MQ=255)                             gTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAat                                                                                                                                                                                                                 >  1:280108/1‑251 (MQ=255)                                             ttGGACATTTGTTTCTCGCCGGCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCAt                                                                                                                                                                                                 <  1:31102/251‑1 (MQ=255)                                                  cATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACggg                                                                                                                                                                                            <  1:763297/251‑1 (MQ=255)                                                   aTTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGc                                                                                                                                                                                           <  1:724720/251‑1 (MQ=255)                                                                cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                                                                                                              <  1:597765/251‑1 (MQ=255)                                                                cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                                                                                                              <  1:80410/251‑1 (MQ=255)                                                                cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                                                                                                              <  1:586470/251‑1 (MQ=255)                                                                        cccACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAg                                                                                                                                                                      >  1:814226/1‑251 (MQ=255)                                                                                     gcggggTAATTGCCACTTATGGTTTTTGACCGGCGGATTTTTGTAACGCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGcc                                                                                                                                                          <  1:385260/247‑1 (MQ=255)                                                                                         ggTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCgg                                                                                                                                                     >  1:552641/1‑251 (MQ=255)                                                                                         ggTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCgg                                                                                                                                                     >  1:669487/1‑251 (MQ=255)                                                                                                 ccACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGt                                                                                                                                             <  1:306405/251‑1 (MQ=255)                                                                                                    cTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttgc                                                                                                                                                                             >  1:508328/1‑216 (MQ=255)                                                                                                                           ttttGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAacac                                                                                                                   <  1:105906/251‑1 (MQ=255)                                                                                                                                tAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATg                                                                                                               <  1:581651/250‑1 (MQ=255)                                                                                                                                  aCTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCgg                                                                                                            >  1:687288/1‑251 (MQ=255)                                                                                                                                  aCTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCgg                                                                                                            >  1:850399/1‑251 (MQ=255)                                                                                                                                     cACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGtt                                                                                                         >  1:551218/1‑251 (MQ=255)                                                                                                                                         aCCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACgg                                                                                                                                                                                      <  1:636980/170‑1 (MQ=255)                                                                                                                                              ccaATAAATTGAATTTTTGGTCGGTCCCGCCCCGTTCAACGTCGGCTTCTAAGGCAACGGTATCCTGCCCTTGCAGAAGCGGATAACTGCATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCggt                                                                                                >  1:421369/1‑250 (MQ=255)                                                                                                                                              ccaATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGa                                                                                                >  1:572954/1‑251 (MQ=255)                                                                                                                                                aaTAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGAt                                                                                               >  1:514264/1‑250 (MQ=255)                                                                                                                                                                     gtcCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGaa                                                                                         <  1:466273/235‑1 (MQ=255)                                                                                                                                                                     gtcCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGaa                                                                                         <  1:877564/235‑1 (MQ=255)                                                                                                                                                                     gtcCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATctg                                                                            >  1:844443/1‑248 (MQ=255)                                                                                                                                                                     gtcCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATctg                                                                            >  1:893575/1‑248 (MQ=255)                                                                                                                                                                     gtcCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATctg                                                                            >  1:500559/1‑248 (MQ=255)                                                                                                                                                                       cccGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTg                                                                                                    >  1:589857/1‑222 (MQ=255)                                                                                                                                                                          gCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGt                                                                                                                          >  1:542707/1‑197 (MQ=255)                                                                                                                                                                           ccAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGt                                                                    <  1:747087/250‑1 (MQ=255)                                                                                                                                                                             aaGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAgg                                                                 <  1:886418/251‑1 (MQ=255)                                                                                                                                                                             aaGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAgg                                                                 <  1:332355/251‑1 (MQ=255)                                                                                                                                                                             aaGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAgg                                                                 <  1:722332/251‑1 (MQ=255)                                                                                                                                                                               gTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCaa                                                                                                                                      <  1:393416/180‑1 (MQ=255)                                                                                                                                                                                     cGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCg                                                         <  1:645305/251‑1 (MQ=255)                                                                                                                                                                                       tCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGt                                                        <  1:136511/250‑1 (MQ=255)                                                                                                                                                                                       tCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGt                                                        <  1:167515/250‑1 (MQ=255)                                                                                                                                                                                       tCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGt                                                        <  1:77557/250‑1 (MQ=255)                                                                                                                                                                                           cTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTgcgc                                                   <  1:626543/251‑1 (MQ=255)                                                                                                                                                                                           cTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTgcg                                                    >  1:353262/1‑250 (MQ=255)                                                                                                                                                                                             tCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGgtgt                                                                                                                                                                                   <  1:354552/121‑1 (MQ=255)                                                                                                                                                                                             tCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCaa                                                 >  1:323101/1‑251 (MQ=255)                                                                                                                                                                                                  ggCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATctgctg                                                                         >  1:294420/1‑222 (MQ=255)                                                                                                                                                                                                   gCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGG‑TGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAg                                                                                                                                     >  1:318000/1‑160 (MQ=255)                                                                                                                                                                                                      aCGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCtt                                         >  1:505534/1‑250 (MQ=255)                                                                                                                                                                                                          aaTCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCgg                                    >  1:586540/1‑251 (MQ=255)                                                                                                                                                                                                           aTCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATg                                                                                                                                                            <  1:800684/130‑1 (MQ=255)                                                                                                                                                                                                           aTCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAac                                                                                                                     <  1:284603/169‑1 (MQ=255)                                                                                                                                                                                                                    ttGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGTCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTc                                           >  1:835967/1‑234 (MQ=255)                                                                                                                                                                                                                     tGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTggcgg                         >  1:704861/1‑251 (MQ=255)                                                                                                                                                                                                                             cGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGggtgg                 <  1:415486/251‑1 (MQ=255)                                                                                                                                                                                                                              ggATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAacac                                                                                                                   >  1:416879/1‑152 (MQ=255)                                                                                                                                                                                                                              ggATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGcgggcggggggg                >  1:760943/1‑247 (MQ=255)                                                                                                                                                                                                                              ggATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGggtggg                >  1:568840/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaaTGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCGACACGTCTTCGCGGCTGAGTGGCGGGCGGGTGGTGttt            <  1:362876/251‑1 (MQ=255)                                                                                                                                                                                                                                      gAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGcgggcgggggggggtttta         >  1:197634/1‑239 (MQ=255)                                                                                                                                                                                                                                       aaTTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGGGTGGTGTTTTTAcc       <  1:841499/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAacac                                              >  1:488530/1‑210 (MQ=255)                                                                                                                                                                                                                                            gTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGGGGGGGGTTTTTACCGGACg  >  1:552645/1‑251 (MQ=255)                                                                                                                                                                                                                                             tGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGcc                                                                                                                                   >  1:312968/1‑121 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATCTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCGATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGATCAAGAATTTTGAAGATCTGCTGTTTGTAGGTTTCTGCGTTGCGCAACACGTCTTCGCGGCTGAGTGGCGGGCGGGTGGTGTTTTTACCGGACG  >  1/932568‑933053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑