Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	932,723	C→T	R192K (AGA→AAA)	unknown ←	Tyrosyl‑tRNA synthetase (EC 6.1.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	932,723	0	C	T	100.0%	199.6 / NA	73	R192K (AGA→AAA)	unknown	Tyrosyl‑tRNA synthetase (EC 6.1.1.1)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/33);  total (40/33)

TTGGATGATTTCCGTTAACGGGCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATCTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCGATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGAT  >  1/932479‑932960                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ttGGATGATTTCCGTTAACGGGCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTggt                                                                                                                                                                                                                                         <  1:383598/251‑1 (MQ=255)   ggATGATTTCCGTTAACGGGCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTggt                                                                                                                                                                                                                                         >  1:842207/1‑249 (MQ=255)            ccGTTAACGGGCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCa                                                                                                                                                                                                                              >  1:21061/1‑251 (MQ=255)            ccGTTAACGGGCGGAAGGAAAGCAGGTTATACCAACCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGCCCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCa                                                                                                                                                                                                                              <  1:356287/251‑1 (MQ=255)                     ggCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTATCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTggtc                                                                                                                                                                                                                                        <  1:442340/232‑1 (MQ=255)                       cGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGt                                                                                                                                                                                                                    <  1:813923/250‑1 (MQ=255)                               aaGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTa                                                                                                                                                                                                           >  1:570369/1‑251 (MQ=255)                                    ggTTATCCCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACTTTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGCCCCACAAATAAATTGAATTTTTGGTCGGTCCCGCCa                                                                                                                                                                                                                              <  1:851920/227‑1 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGt                                                                                                                                                                                                                    >  1:515861/1‑237 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAg                                                                                                                                                                                                         <  1:96496/248‑1 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCa                                                                                                                                                                                                      >  1:738753/1‑251 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCa                                                                                                                                                                                                      >  1:535280/1‑251 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCa                                                                                                                                                                                                      >  1:587759/1‑251 (MQ=255)                                    ggTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCa                                                                                                                                                                                                      >  1:188573/1‑251 (MQ=255)                                     gTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGc                                                                                                                                                                                                       <  1:767423/249‑1 (MQ=255)                                     gTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGc                                                                                                                                                                                                       <  1:432977/249‑1 (MQ=255)                                     gTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCaa                                                                                                                                                                                                     >  1:616222/1‑251 (MQ=255)                                        ataCCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACgg                                                                                                                                                                                                  >  1:82176/1‑251 (MQ=255)                                         taCCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGa                                                                                                                                                                                                 <  1:475919/251‑1 (MQ=255)                                               tCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATc                                                                                                                                                                                              >  1:626586/1‑248 (MQ=255)                                                   acaTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGc                                                                                                                                                                                                       <  1:839724/235‑1 (MQ=255)                                                   acaTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGcc                                                                                                                                                                                         >  1:381600/1‑249 (MQ=255)                                                    caTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCtt                                                                                                                                                                                      >  1:265965/1‑251 (MQ=255)                                                    caTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCtt                                                                                                                                                                                      >  1:545575/1‑251 (MQ=255)                                                    caTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCtt                                                                                                                                                                                      >  1:321228/1‑251 (MQ=255)                                                    caTTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGCGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCtt                                                                                                                                                                                      >  1:90728/1‑251 (MQ=255)                                                                 gAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGAt                                                                                                                                                                          <  1:506417/250‑1 (MQ=255)                                                                     tGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATg                                                                                                                                                                     <  1:772280/251‑1 (MQ=255)                                                                           tGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCg                                                                                                                                                               >  1:370157/1‑251 (MQ=255)                                                                             aTTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTg                                                                                                                                                             <  1:900800/251‑1 (MQ=255)                                                                                ttACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAAt                                                                                                                                                          <  1:686093/251‑1 (MQ=255)                                                                                ttACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAAt                                                                                                                                                          >  1:637361/1‑251 (MQ=255)                                                                                   ccGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGc                                                                                                                                                       <  1:815189/251‑1 (MQ=255)                                                                                     gAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTg                                                                                                                                                                                           <  1:315842/213‑1 (MQ=255)                                                                                          tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                <  1:430431/251‑1 (MQ=255)                                                                                          tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                <  1:283770/251‑1 (MQ=255)                                                                                          tttCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggt                                                                                                                                                <  1:282850/251‑1 (MQ=255)                                                                                               cTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCggttggtt                                                                                                                                           <  1:653849/251‑1 (MQ=255)                                                                                                   gTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTAttt                                                                                                                                       >  1:796117/1‑251 (MQ=255)                                                                                                   gTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTAttt                                                                                                                                       >  1:388845/1‑251 (MQ=255)                                                                                                    tGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTg                                                                                                                                      <  1:866425/251‑1 (MQ=255)                                                                                                         ccGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGc                                                                                                                                                                                                       >  1:618420/1‑181 (MQ=255)                                                                                                                 ccGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGtttttt                                                                                                                         >  1:847647/1‑251 (MQ=255)                                                                                                                    aTGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGaa                                                                                                                      >  1:50078/1‑251 (MQ=255)                                                                                                                      gTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAat                                                                                                                    >  1:280108/1‑251 (MQ=255)                                                                                                                      gTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAat                                                                                                                    >  1:529145/1‑251 (MQ=255)                                                                                                                           tGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGc                                                                                                                                                                                                       >  1:210890/1‑163 (MQ=255)                                                                                                                                  ggATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAg                                                                                                                                                                               >  1:686597/1‑180 (MQ=255)                                                                                                                                      ttGGACATTTGTTTCTCGCCGGCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCAt                                                                                                    <  1:31102/251‑1 (MQ=255)                                                                                                                                        ggACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAgg                                                                                                                                                                                                        >  1:437839/1‑149 (MQ=255)                                                                                                                                           cATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACggg                                                                                               <  1:763297/251‑1 (MQ=255)                                                                                                                                            aTTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGc                                                                                              <  1:724720/251‑1 (MQ=255)                                                                                                                                                         cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                 <  1:597765/251‑1 (MQ=255)                                                                                                                                                         cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                 <  1:80410/251‑1 (MQ=255)                                                                                                                                                         cGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttg                                                                                 <  1:586470/251‑1 (MQ=255)                                                                                                                                                                 cccACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAg                                                                         >  1:814226/1‑251 (MQ=255)                                                                                                                                                                              gcggggTAATTGCCACTTATGGTTTTTGACCGGCGGATTTTTGTAACGCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGcc                                                             <  1:385260/247‑1 (MQ=255)                                                                                                                                                                                 gggTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGc                                                                                                                                                                                    >  1:104887/1‑128 (MQ=255)                                                                                                                                                                                  ggTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCgg                                                        >  1:669487/1‑251 (MQ=255)                                                                                                                                                                                  ggTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCgg                                                        >  1:552641/1‑251 (MQ=255)                                                                                                                                                                                          ccACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGt                                                <  1:306405/251‑1 (MQ=255)                                                                                                                                                                                             cTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAAttgc                                                                                >  1:508328/1‑216 (MQ=255)                                                                                                                                                                                                                    ttttGTAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAacac                      <  1:105906/251‑1 (MQ=255)                                                                                                                                                                                                                         tAACTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATg                  <  1:581651/250‑1 (MQ=255)                                                                                                                                                                                                                           aCTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAg                                                                                                                                                        >  1:589632/1‑114 (MQ=255)                                                                                                                                                                                                                           aCTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCgg               >  1:687288/1‑251 (MQ=255)                                                                                                                                                                                                                           aCTCACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCgg               >  1:850399/1‑251 (MQ=255)                                                                                                                                                                                                                              cACGACCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGtt            >  1:551218/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCCCACCAATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACgg                                                                                         <  1:636980/170‑1 (MQ=255)                                                                                                                                                                                                                                       ccaATAAATTGAATTTTTGGTCGGTCCCGCCCCGTTCAACGTCGGCTTCTAAGGCAACGGTATCCTGCCCTTGCAGAAGCGGATAACTGCATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCggt   >  1:421369/1‑250 (MQ=255)                                                                                                                                                                                                                                       ccaATAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGa   >  1:572954/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaTAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCTATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGAt  >  1:514264/1‑250 (MQ=255)                                                                                                                                                                                                                                          aTAAATTGAATTTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAAtt                                                                                                                                                                 <  1:279981/90‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              TTGGATGATTTCCGTTAACGGGCGGAAGGAAAGCAGGTTATACCAATCCCACATTAGATCATCGGAAATGGACATGATTTTACCGAACATTTCGCTCGGTGCGTCCGTTACGCCGATGTAATTGCCAAGGGATTTGGACATTTTTTTCTCGCCGTCCAAGCCCACCAACAGCGGCAGGGTAATTGCCACTTGTGGTTTTTGACCGGCGGATTTTTGTAACTCACGACCCACCAATAAATTGAATCTTTGGTCGGTCCCGCCAAGTTCAACGTCGGCTTCTAAGGCAACGGAATCCTGCCCTTGCAGAAGCGGATAAATGAATTCGTGAATAGCGATCGGTTGGTTATTTGAAAAGCGTTTTTTGAAATCATCACGTTCCAGCATACGGGCAACGGTGTAATTGCTTGCCAGGCGAATCATGCCTTCGGTGCCGAGTTTACCAAGCCAGTCGGAGTTAAACACGATGCGGGTTTTTTGCGGAT  >  1/932479‑932960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑