Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	919,376	T→C	V9A (GTA→GCA)	unknown →	Transcription termination protein NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	919,376	0	T	C	100.0%	180.4 / NA	65	V9A (GTA→GCA)	unknown	Transcription termination protein NusA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/28);  total (37/28)

TACCTTTGCACAATATGCCCGTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGTAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAGATCCTGACG  >  1/919132‑919609                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          tACCTTGGCGCAATATGCCCGTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGc                                                                                                                                                                                                                                     <  1:880240/251‑1 (MQ=255)         cgcaATATGCCCGTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATcc                                                                                                                                                                                                                              <  1:862568/248‑1 (MQ=255)           caATATGCCCGTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAAt                                                                                                                                                                                                                           >  1:669052/1‑251 (MQ=255)                     gTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAattat                                                                                                                                                                                                                 >  1:870438/1‑251 (MQ=255)                                  ggAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATcc                                                                                                                                                                                                                              >  1:584955/1‑225 (MQ=255)                                  ggAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGaa                                                                                                                                                                                                    >  1:50848/1‑251 (MQ=255)                                  ggAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGaa                                                                                                                                                                                                    >  1:6413/1‑251 (MQ=255)                                            gTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATcc                                                                                                                                                                                                                              >  1:613580/1‑215 (MQ=255)                                            gTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGaa                                                                                                                                                                                          >  1:653932/1‑251 (MQ=255)                                                    gcgcATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGa                                                                                                                                                                                  >  1:365621/1‑251 (MQ=255)                                                         ttCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTg                                                                                                                                                                             >  1:707983/1‑251 (MQ=255)                                                            cGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCaa                                                                                                                                                                          <  1:359964/251‑1 (MQ=255)                                                                             aaaTGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAg                                                                                                                                                         <  1:171564/251‑1 (MQ=255)                                                                                tGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAAc                                                                                                                                                             <  1:399183/244‑1 (MQ=255)                                                                                tGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGaaa                                                                                                                                                      <  1:769145/251‑1 (MQ=255)                                                                                tGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGaaa                                                                                                                                                      >  1:198020/1‑251 (MQ=255)                                                                                                cGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACg                                                                                                                                      <  1:555512/251‑1 (MQ=255)                                                                                                      ttGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATg                                                                                                                                <  1:627206/251‑1 (MQ=255)                                                                                                      ttGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATg                                                                                                                                <  1:773708/251‑1 (MQ=255)                                                                                                      ttGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATg                                                                                                                                <  1:465991/251‑1 (MQ=255)                                                                                                                  tgatTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATa                                                                                                                                                 <  1:109013/222‑1 (MQ=255)                                                                                                                  tgatTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAata                                                                                                                                           >  1:34753/1‑228 (MQ=255)                                                                                                                  tgatTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCa                                                                                                                    >  1:850792/1‑251 (MQ=255)                                                                                                                       aCGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCTAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAAt                                                                                                               <  1:769665/251‑1 (MQ=255)                                                                                                                        cGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAAcc                                                                                                                                                            <  1:500047/205‑1 (MQ=255)                                                                                                                        cGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAAt                                                                                                               >  1:502565/1‑250 (MQ=255)                                                                                                                           tGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGAGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCaa                                                                                                           <  1:540882/251‑1 (MQ=255)                                                                                                                            gATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCaaa                                                                                                          >  1:238732/1‑251 (MQ=255)                                                                                                                            gATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCaaa                                                                                                          >  1:329542/1‑251 (MQ=255)                                                                                                                                  ggATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCgg                                                                                                    >  1:865611/1‑251 (MQ=255)                                                                                                                                           ggAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGa                                                                                           >  1:60901/1‑251 (MQ=255)                                                                                                                                            gAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGa                                                                                           >  1:361987/1‑250 (MQ=255)                                                                                                                                               cAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATcc                                                                                                             >  1:731/1‑229 (MQ=255)                                                                                                                                                 gATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCtt                                                                                     >  1:612626/1‑251 (MQ=255)                                                                                                                                                 gATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCt                                                                                      >  1:213760/1‑250 (MQ=255)                                                                                                                                                             cGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGt                                                                         <  1:331093/251‑1 (MQ=255)                                                                                                                                                                   tatTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTgtggt                                                                   >  1:469687/1‑251 (MQ=255)                                                                                                                                                                    atTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAg                                                                                                                                                                                         <  1:551833/132‑1 (MQ=255)                                                                                                                                                                    atTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTgtggtg                                                                  >  1:187060/1‑251 (MQ=255)                                                                                                                                                                       cAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGgaa                                                               <  1:373462/251‑1 (MQ=255)                                                                                                                                                                       cAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGgaa                                                               <  1:480412/251‑1 (MQ=255)                                                                                                                                                                       cAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGgaa                                                               <  1:137483/251‑1 (MQ=255)                                                                                                                                                                              cTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTg                                                         <  1:866520/250‑1 (MQ=255)                                                                                                                                                                                        cgcgAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGa                                               >  1:470463/1‑250 (MQ=255)                                                                                                                                                                                                  taaatttaaaGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATc                                    <  1:767164/251‑1 (MQ=255)                                                                                                                                                                                                              agGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAagca                        >  1:214852/1‑251 (MQ=255)                                                                                                                                                                                                               ggATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGaagaag                                                           >  1:833410/1‑215 (MQ=255)                                                                                                                                                                                                               ggATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGaataa                                                    >  1:594411/1‑222 (MQ=255)                                                                                                                                                                                                               ggATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTg                              >  1:790275/1‑244 (MQ=255)                                                                                                                                                                                                                gATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATg                                                                                                                                >  1:363338/1‑145 (MQ=255)                                                                                                                                                                                                                gATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCcgtc                                                                                <  1:717226/193‑1 (MQ=255)                                                                                                                                                                                                                gATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGaa                           <  1:631976/246‑1 (MQ=255)                                                                                                                                                                                                                gATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAagcag                       >  1:656235/1‑250 (MQ=255)                                                                                                                                                                                                                gATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAagcag                       >  1:523384/1‑250 (MQ=255)                                                                                                                                                                                                                    gAAACAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCg                                                                                                                                                                       >  1:648631/1‑102 (MQ=255)                                                                                                                                                                                                                     aaaCAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGtt                 >  1:217304/1‑251 (MQ=255)                                                                                                                                                                                                                      aaCAGCATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGttt                >  1:278269/1‑251 (MQ=255)                                                                                                                                                                                                                           cATGAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAg           <  1:738855/251‑1 (MQ=255)                                                                                                                                                                                                                              gAGTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGaaa                                      <  1:568707/221‑1 (MQ=255)                                                                                                                                                                                                                                gTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAg                                         <  1:789004/216‑1 (MQ=255)                                                                                                                                                                                                                                gTAAAGAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAGATc        >  1:840259/1‑249 (MQ=255)                                                                                                                                                                                                                                     gAAATTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAGATCCTGAcg  >  1:574168/1‑250 (MQ=255)                                                                                                                                                                                                                                       aaTTTTATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAagc                         <  1:125927/225‑1 (MQ=255)                                                                                                                                                                                                                                         ttttATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGacac                                                                                        >  1:53722/1‑160 (MQ=255)                                                                                                                                                                                                                                          tttATTAGCGGCAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAg           <  1:84881/236‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          TACCTTTGCACAATATGCCCGTTATGTTGGGCAGGAAATCTTGGTGCATTTGCGCATTCCGGTGGCGGATCGTCGTAAATGGCAAGGGGAATTGGCGAAAATTGAAAATGATATGATTACGCTGATTGTGGATAAACAGGAACAGATTTTAGCGTTCGGTAATATTCAAAAAGCTAGCGTTGTCGCGAAATTTTAAATTTAAAGAAGGATAGAAACAGCATGAGTAAAGAAATTTTATTAGCGGTAGAAGCCGTATCCAATGAAAAATTATTGCCACGCGAGAAAATTTTTGAAGCGTTGGAAAGTGCAATCGCCCTCTCAACCAAGAAAAAATACGAATATGACGTAGATGTTCGCGTCGCCATTAATCCAAAAACCGGCGAATTCGACACCTTCCGTCGTTGGGTTGTGGTGGAAGAAGTGAATAATCCGACTAAAGAAATCACGTTGGAAGCAGCACGTTTTGAAGATCCTGACG  >  1/919132‑919609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑