Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	917,849	A→G	T195T (ACT→ACC)	unknown ←	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	917,849	0	A	G	100.0%	209.7 / NA	75	T195T (ACT→ACC)	unknown	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/36);  total (39/36)

TGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTAGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGAAAATAGGCGAACAC  >  1/917605‑918085                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             tGATAAAACACAGAATAATGATGGTCACGATGTGCGGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTg                                                                                                                                                                                                                                           >  1:267986/1‑248 (MQ=255) tGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCg                                                                                                                                                                                                                                        >  1:230546/1‑251 (MQ=255)  gATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGa                                                                                                                                                                                                                                       >  1:254632/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:145795/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:813586/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:581948/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:44430/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:361850/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:147930/1‑251 (MQ=255)             gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                                                                                            >  1:197964/1‑251 (MQ=255)                      tgGTCACGCTGAGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATACAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATggg                                                                                                                                                                                                                   >  1:256070/1‑251 (MQ=255)                            cGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTgg                                                                                                                                                                                                             >  1:884120/1‑251 (MQ=255)                              aTGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGga                                                                                                                                                                                                           >  1:233364/1‑251 (MQ=255)                               tgtgCAGTTTCGGTAACCCGAATTTGCCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGgag                                                                                                                                                                                                          <  1:356362/251‑1 (MQ=255)                               tgtgCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGgag                                                                                                                                                                                                          <  1:257710/251‑1 (MQ=255)                                      tttCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTc                                                                                                                                                                                                     <  1:213639/249‑1 (MQ=255)                                               cccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGc                                                                                                                                                                                                                                          >  1:381837/1‑203 (MQ=255)                                                ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                                                                                         <  1:748809/251‑1 (MQ=255)                                                ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                                                                                         <  1:855146/251‑1 (MQ=255)                                                ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                                                                                         <  1:842421/251‑1 (MQ=255)                                                   aaTTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAAcaccac                                                                                                                                                                                      <  1:627861/251‑1 (MQ=255)                                                    aTTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACcaca                                                                                                                                                                                     <  1:461802/251‑1 (MQ=255)                                                        aCCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCAc                                                                                                                                                                                 <  1:711255/251‑1 (MQ=255)                                                           aTCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCgg                                                                                                                                                                              <  1:27155/251‑1 (MQ=255)                                                               aaaaGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCAc                                                                                                                                                                                 <  1:399006/244‑1 (MQ=255)                                                                 aaGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCg                                                                                                                                                                        >  1:216900/1‑251 (MQ=255)                                                                           ccGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGc                                                                                                                                                                                                                                          >  1:281134/1‑175 (MQ=255)                                                                                   gggTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGGAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGaaa                                                                                                                                                      >  1:453919/1‑251 (MQ=255)                                                                                   gggTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGaaa                                                                                                                                                      >  1:611969/1‑251 (MQ=255)                                                                                     gTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTCCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAAt                                                                                                                                                     <  1:425538/250‑1 (MQ=255)                                                                                            cGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGCGACAAAATAATGGAAATCGGTGAgg                                                                                                                                             >  1:730412/1‑251 (MQ=255)                                                                                                  aaTCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACaaa                                                                                                                                       <  1:211627/251‑1 (MQ=255)                                                                                                        gTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATc                                                                                                                                                                                   >  1:554234/1‑201 (MQ=255)                                                                                                        gTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCgtaaaacgaatgtccgtg                                                                                                                                  >  1:882511/1‑233 (MQ=255)                                                                                                                 cGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCAc                                                                                                                        <  1:31129/251‑1 (MQ=255)                                                                                                                 cGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCAc                                                                                                                        <  1:36539/251‑1 (MQ=255)                                                                                                                         tGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCgagag                                                                                                                <  1:779065/251‑1 (MQ=255)                                                                                                                         tGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCgagag                                                                                                                <  1:703168/251‑1 (MQ=255)                                                                                                                           gTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTggg                                                                                                                                                                                                            >  1:780799/1‑157 (MQ=255)                                                                                                                                        aCGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGAcc                                                                                                 <  1:449245/251‑1 (MQ=255)                                                                                                                                           cgTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATgg                                                                                                                                                         >  1:205344/1‑192 (MQ=255)                                                                                                                                           cgTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGAcc                                                                                                 <  1:579996/248‑1 (MQ=255)                                                                                                                                             tCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGAc                                                                                            <  1:403890/251‑1 (MQ=255)                                                                                                                                                    gTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGAt                                                                                                                                                                                                                 >  1:852131/1‑127 (MQ=255)                                                                                                                                                     ttCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAAc                                                                                     <  1:825627/250‑1 (MQ=255)                                                                                                                                                     ttCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAAc                                                                                     <  1:630617/250‑1 (MQ=255)                                                                                                                                                         aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGAt                                                                                                                                                                                                                 >  1:779963/1‑122 (MQ=255)                                                                                                                                                         aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGt                                                                                                                                                                          >  1:810585/1‑161 (MQ=255)                                                                                                                                                         aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGt                                                                                >  1:283661/1‑251 (MQ=255)                                                                                                                                                                      cttTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCGCCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAAt                                                                   <  1:474270/251‑1 (MQ=255)                                                                                                                                                                      cttTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAAt                                                                   >  1:437084/1‑251 (MQ=255)                                                                                                                                                                        ttGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATAc                                                                 <  1:204977/251‑1 (MQ=255)                                                                                                                                                                            gATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGc                                                               >  1:656779/1‑249 (MQ=255)                                                                                                                                                                             aTCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATggg                                                                                                                                                                                                                   >  1:677796/1‑100 (MQ=255)                                                                                                                                                                               ccAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTaaa                                                          >  1:99603/1‑251 (MQ=255)                                                                                                                                                                               ccAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTaaa                                                          >  1:157474/1‑251 (MQ=255)                                                                                                                                                                                cAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAc                                                         <  1:26691/251‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAgg                                                                                                                                                                           <  1:408270/136‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGAc                                                                                                        <  1:655952/203‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGt                                                                                <  1:187715/227‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACaa                                                                    >  1:825967/1‑239 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAcc                                                        <  1:505346/251‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAcc                                                        <  1:46303/251‑1 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAc                                                         >  1:702933/1‑250 (MQ=255)                                                                                                                                                                                 aaaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAc                                                         >  1:694859/1‑250 (MQ=255)                                                                                                                                                                                  aaTAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAAc                                                         >  1:188784/1‑249 (MQ=255)                                                                                                                                                                                               gAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGa                                          >  1:212704/1‑251 (MQ=255)                                                                                                                                                                                                                cGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAgg                         <  1:264091/251‑1 (MQ=255)                                                                                                                                                                                                                cGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAg                          <  1:516688/250‑1 (MQ=255)                                                                                                                                                                                                                    gCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATgg                                                                                                                                                         >  1:757098/1‑119 (MQ=255)                                                                                                                                                                                                                           aTGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGaaa              <  1:37231/251‑1 (MQ=255)                                                                                                                                                                                                                              gCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGAAAAt            <  1:131570/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGAAAATAgg         <  1:555667/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCa                                                                           >  1:204434/1‑182 (MQ=255)                                                                                                                                                                                                                                        aaaCCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGAAAATAGGCGAacac  <  1:250494/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             TGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTAGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGCCTAAACCTGTGGCAGAGGCGACCGCAAAGGACATCAGGCACGCTAGAAAATAGGCGAACAC  >  1/917605‑918085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑