Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	911,419	G→A	intergenic (‑189/‑66)	unknown ← / → unknown	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	911,419	0	G	A	100.0%	78.6 / NA	31	intergenic (‑189/‑66)	unknown/unknown	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/11);  total (20/11)

GAATTCACTGTATTTCATCGGGGTACCTCCTTTGCTATACAGTGAGGATAGAATACAACAAATTTGTTGTATGAGCAACATTTTTGTTGTGTTTTTTAAGTGATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAGAACTTACGGAAATGTCTTGGGTTTTGTAGAATGGGCGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATCGTTATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTCAAGAAAATCAAACAGCGTAGAGGGCAAAAAACGTATTTAAAATCGGTAT  >  1/911213‑911614                                                                                                                                                                                                               |                                                                                                                                                                                                    gAATTCACTGTATTTCATCGGGGTACCTCCTTTGCTATACAGTGAAGATAGAATACAACAAATTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTAt                                                                                                                                                         >  1:106857/1‑251 (MQ=255)                     gggTACCTCCTTTGCTATACAGTGAAGATAGAATACAACAAATTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACatat                                                                                                                                      >  1:671737/1‑250 (MQ=255)                                  gCTATACAGTGAAGATAGAATACAACAAATTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTAt                                                                                                                                                         >  1:120873/1‑218 (MQ=255)                                              agATAGAATACAACAAATTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGt                                                                                                                                                                               >  1:568196/2‑184 (MQ=255)                                                       acaacaAATTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTAtt                                                                                                   >  1:355306/1‑250 (MQ=255)                                                            aaaTTTGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTAttgtcat                                                                                              <  1:439048/251‑7 (MQ=255)                                                               tttGTTATATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACatt                                                                                                                  >  1:389025/1‑228 (MQ=255)                                                                    tatATGTGCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCattttaattt                                                                                      >  1:465795/3‑251 (MQ=255)                                                                         gtgCAACATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATg                                                                                  >  1:86253/3‑250 (MQ=255)                                                                              aCATTTTTGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACatt                                                                                                                  >  1:340025/1‑213 (MQ=255)                                                                                 tttttGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAGATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGc                                                                                 >  1:690152/1‑243 (MQ=255)                                                                                 tttttGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCttt                                                                         >  1:717576/1‑251 (MQ=255)                                                                                  ttttGTTGTGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCtttt                                                                        >  1:765060/1‑251 (MQ=255)                                                                                       ttgtGTTTTTTAAATAATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCAt                                                                    <  1:665719/250‑1 (MQ=255)                                                                                           gtTTTTTAAATAATGATTTATTTGATAACCTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGAtt                                                               >  1:342690/1‑251 (MQ=255)                                                                                                        tgatTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGtga                                                  <  1:837702/251‑3 (MQ=255)                                                                                                         gatTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTg                                                                                                              <  1:471460/190‑1 (MQ=255)                                                                                                                           ttttCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAACAGCGTag                               <  1:467685/251‑1 (MQ=255)                                                                                                                            tttCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAACAGCGTaga                              >  1:9315/1‑251 (MQ=255)                                                                                                                              tCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAGCACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAAc                                      <  1:756041/241‑1 (MQ=255)                                                                                                                               cAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGAt                                                       >  1:213759/1‑223 (MQ=255)                                                                                                                               cAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAACAGCGTAGAggg                           <  1:569146/251‑1 (MQ=255)                                                                                                                                   aGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGGACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGAt                                                       <  1:408488/219‑1 (MQ=255)                                                                                                                                               aCGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGc                                                                      <  1:73813/192‑1 (MQ=255)                                                                                                                                                        tttttAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAACAGCGTAGAGGGCaaaaggcattaaatgattatcaag  <  1:900277/251‑21 (MQ=255)                                                                                                                                                              ataatGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCattttaat                                                                                        >  1:644845/1‑159 (MQ=255)                                                                                                                                                              ataatGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGc                                                                      <  1:69616/177‑1 (MQ=255)                                                                                                                                                                aatGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAAc                                                                                                                                          >  1:436880/1‑107 (MQ=255)                                                                                                                                                                        cTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAAtaggag                                                                                                                                                                >  1:833559/1‑72 (MQ=255)                                                                                                                                                                        cTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATc                                                                                                                                     >  1:210695/1‑104 (MQ=255)                                                                                                                                                                        cTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAAAACTTACGGAAATGTCTTGGGTTTCGTAAAATAGGAGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATTGTCATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTGAAAAAAATCAAACAGCGTAGAGGGCaaaaggcattaaatg           >  1:458755/1‑215 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                    GAATTCACTGTATTTCATCGGGGTACCTCCTTTGCTATACAGTGAGGATAGAATACAACAAATTTGTTGTATGAGCAACATTTTTGTTGTGTTTTTTAAGTGATGATTTATTTGATAAACTTTTTTCAAAAGCCTGTTTAAAACGTAGGTTTTTTTAATAATGGAATCTGCGACGCAGATCGCAAAATTGAAATAAATTTGAGGGAGAACTTACGGAAATGTCTTGGGTTTTGTAGAATGGGCGTTTCTATTTTTTACTAAGGAACATATCTATGAACACAACCAACATTATTGCCGGAATTATCGTTATTTTAATTTTATGCAGCCCTTTTGCATTGATTTACTGGATAGTCAAGAAAATCAAACAGCGTAGAGGGCAAAAAACGTATTTAAAATCGGTAT  >  1/911213‑911614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑