Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	910,931	G→A	D22D (GAC→GAT)	unknown ←	Antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	910,931	0	G	A	100.0%	168.2 / NA	62	D22D (GAC→GAT)	unknown	Antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/33);  total (29/33)

TGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCACTCATTCAACGTTACCTTGCGATGACTTCCGCGCTTGGTTG  >  1/910684‑911175                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     tgtgAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCg                                                                                                                                                                                                                                                   >  1:320440/1‑251 (MQ=255)                  cgcgcCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGaa                                                                                                                                                                                                                                  <  1:392859/251‑1 (MQ=255)                         tttCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                                                                                                                                                                                           <  1:598579/251‑1 (MQ=255)                                  ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                                                                                                                                                                                           <  1:199088/242‑1 (MQ=255)                                  ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATcc                                                                                                                                                                                                                  <  1:298645/251‑1 (MQ=255)                                    cGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCgg                                                                                                                                                                                                                <  1:777076/251‑1 (MQ=255)                                          ccTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAgg                                                                                                                                                                                                                                    <  1:614403/225‑1 (MQ=255)                                                 cGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAAc                                                                                                                                                                                                    <  1:246761/250‑1 (MQ=255)                                                     cATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGt                                                                                                                                                                                               <  1:396053/251‑1 (MQ=255)                                                       tttCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTc                                                                                                                                                                                                       <  1:676605/241‑1 (MQ=255)                                                              gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                                                                                                                                                                      >  1:511924/1‑251 (MQ=255)                                                              gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                                                                                                                                                                      >  1:84541/1‑251 (MQ=255)                                                              gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                                                                                                                                                                      >  1:615092/1‑251 (MQ=255)                                                               aGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                                                                                                                                                                     >  1:872266/1‑251 (MQ=255)                                                                    gaagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTatcatc                                                                                                                                                                                                                   <  1:804038/216‑1 (MQ=255)                                                                     aagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGttt                                                                                                                                                                                <  1:313335/250‑1 (MQ=255)                                                                         cTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTtctc                                                                                                                                                                             >  1:174844/1‑249 (MQ=255)                                                                                tGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATc                                                                                                                                                                     <  1:627571/250‑1 (MQ=255)                                                                                          aaGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGt                                                                                                                                                                                          >  1:392324/1‑219 (MQ=255)                                                                                            ggAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                                                                                                                                                                     <  1:760670/222‑1 (MQ=255)                                                                                                      accgtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCa                                                                                                                                                                                                                                              <  1:364938/151‑1 (MQ=255)                                                                                                      accgtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                                                                                                                                                                     >  1:493451/5‑212 (MQ=255)                                                                                                         gtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGt                                                                                                                                                                                               <  1:777079/198‑1 (MQ=255)                                                                                                         gtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCagag                                                                                                                                           >  1:662233/2‑251 (MQ=255)                                                                                                          tatattggccATCTTTTGGGTGGCTTGGGATTGGAATTTGGCGTTTATCCACAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAgg                                                                                                                                          <  1:715248/243‑1 (MQ=255)                                                                                                          tataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAgg                                                                                                                                          <  1:746871/251‑1 (MQ=255)                                                                                                                gCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAg                                                                                                                                    >  1:581274/1‑251 (MQ=255)                                                                                                                     tCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGAc                                                                                                                                  >  1:393093/1‑248 (MQ=255)                                                                                                                      cTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGt                                                                                                                                                                                          >  1:594050/1‑191 (MQ=255)                                                                                                                            gctggctTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACG‑CCCTTTTCAGAGGCGAAAGACTTATtttaag                                                                                                                        <  1:253186/250‑1 (MQ=255)                                                                                                                             ctggctTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATtttaagt                                                                                                                       <  1:203029/251‑1 (MQ=255)                                                                                                                               ggctTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATtttaagttt                                                                                                                     >  1:301568/1‑251 (MQ=255)                                                                                                                                     gCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTttaagtttaagt                                                                                                                 >  1:78212/1‑249 (MQ=255)                                                                                                                                          ggAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCtttttt                                                                                                          >  1:291737/1‑251 (MQ=255)                                                                                                                                          ggAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCtttttt                                                                                                          >  1:261624/1‑251 (MQ=255)                                                                                                                                           gAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTc                                                                                                                                               >  1:885474/1‑213 (MQ=255)                                                                                                                                                        atcatcAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTa                                                                                            >  1:640079/1‑251 (MQ=255)                                                                                                                                                                                 gTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTtactac                                                                    >  1:736336/1‑250 (MQ=255)                                                                                                                                                                                 gTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTtactac                                                                    <  1:266613/250‑1 (MQ=255)                                                                                                                                                                                 gTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTtactac                                                                    <  1:14732/250‑1 (MQ=255)                                                                                                                                                                                     cTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATgg                                                               <  1:548158/251‑1 (MQ=255)                                                                                                                                                                                                tcCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGAc                                                    <  1:212146/251‑1 (MQ=255)                                                                                                                                                                                                  cGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAg                                                  <  1:477110/251‑1 (MQ=255)                                                                                                                                                                                                   gCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAgg                                                                                                                                                                                                                                    <  1:893719/72‑1 (MQ=255)                                                                                                                                                                                                   gCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATtttaag                                                                                                                        >  1:155296/1‑180 (MQ=255)                                                                                                                                                                                                   gCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGt                                                              <  1:637412/238‑1 (MQ=255)                                                                                                                                                                                                   gCTTCAAGCAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGCGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAATCCGGTACCGATTTCTTTACTACCATGGTATGGTAAGACAGt                                                 >  1:561371/1‑251 (MQ=255)                                                                                                                                                                                                                 gcTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCa                                   >  1:30772/1‑251 (MQ=255)                                                                                                                                                                                                                    ttCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAggg                                                          <  1:712291/225‑1 (MQ=255)                                                                                                                                                                                                                     tCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTGCCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGt                               <  1:523086/251‑1 (MQ=255)                                                                                                                                                                                                                     tCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTGCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGt                               <  1:423024/251‑1 (MQ=255)                                                                                                                                                                                                                          atCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTAcc                                                                                  >  1:199897/1‑195 (MQ=255)                                                                                                                                                                                                                          atCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTAc                                                                                   <  1:152078/194‑1 (MQ=255)                                                                                                                                                                                                                          atCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGt                                                              <  1:601015/215‑1 (MQ=255)                                                                                                                                                                                                                               caacTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAAc                                                                                                                                                                                                    <  1:164594/73‑1 (MQ=255)                                                                                                                                                                                                                               cACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCa                                                                  >  1:854654/1‑206 (MQ=255)                                                                                                                                                                                                                               cACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGTTACCTTGCGa                     >  1:521402/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCag                                          >  1:703086/1‑227 (MQ=255)                                                                                                                                                                                                                                    acacTTACCATTTCGGGTATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGAc                                                    >  1:284718/1‑215 (MQ=255)                                                                                                                                                                                                                                    acacTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGTTACCTTGCGATGa                  >  1:629/1‑249 (MQ=255)                                                                                                                                                                                                                                           ccATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGTTACCTTGCGATGACTTc              <  1:727570/246‑1 (MQ=255)                                                                                                                                                                                                                                                  gggAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGAAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCAGTCATTCAACGTTACCTTGCGATGACTTCCGCGCTTGGTTg  >  1:603724/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     TGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGGCGAAAGACTTATTTTAAGTTTAAGTCTTTTTTGATTTTGTGAACTAAGCCGGTACCGATTTCTTTACTACCATGGTAGGGAAAGACAGTTTGGCACTCATTCAACGTTACCTTGCGATGACTTCCGCGCTTGGTTG  >  1/910684‑911175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑