Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	910,793	C→T	Q68Q (CAG→CAA)	unknown ←	Antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	910,793	0	C	T	100.0%	152.9 / NA	54	Q68Q (CAG→CAA)	unknown	Antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/32);  total (22/32)

CTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGG  >  1/910547‑911039                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGcc                                                                                                                                                                                                                                                    <  1:134821/251‑1 (MQ=255)       gTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCtt                                                                                                                                                                                                                                              >  1:180539/1‑251 (MQ=255)                           ttttttAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGa                                                                                                                                                                                                                          >  1:520124/1‑251 (MQ=255)                                  aTGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGc                                                                                                                                                                                                                   <  1:633003/251‑1 (MQ=255)                                   tGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCg                                                                                                                                                                                                                  <  1:868643/251‑1 (MQ=255)                                       ccGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAAACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTa                                                                                                                                                                                                              >  1:445081/1‑251 (MQ=255)                                         gATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTat                                                                                                                                                                                                             >  1:496607/1‑250 (MQ=255)                                                          cccTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCaa                                                                                                                                                                                           >  1:882473/1‑251 (MQ=255)                                                                          gCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAc                                                                                                                                                                            <  1:224717/250‑1 (MQ=255)                                                                           cTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAcc                                                                                                                                                                           <  1:556907/250‑1 (MQ=255)                                                                                          cAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                                           <  1:283375/251‑1 (MQ=255)                                                                                                           cGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATc                                                                                                                                          <  1:26603/251‑1 (MQ=255)                                                                                                              aaaTCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCtt                                                                                                                                        >  1:673810/1‑250 (MQ=255)                                                                                                                 tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGTCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                                           >  1:760461/1‑228 (MQ=255)                                                                                                                 tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCttcttc                                                                                                                                                                     <  1:828676/218‑1 (MQ=255)                                                                                                                 tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                                           <  1:309282/228‑1 (MQ=255)                                                                                                                      taaGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTacac                                                                                                                               <  1:759994/251‑1 (MQ=255)                                                                                                                                        cATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAc                                                                                                                                                                            <  1:457408/188‑1 (MQ=255)                                                                                                                                          tgtgAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCg                                                                                                           >  1:320440/1‑251 (MQ=255)                                                                                                                                                cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGaa                                                                                                                                                                                                                         >  1:229456/1‑135 (MQ=255)                                                                                                                                                cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCAcc                                                                                                                                    <  1:17590/220‑1 (MQ=255)                                                                                                                                                cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTacac                                                                                                                               >  1:780562/1‑225 (MQ=255)                                                                                                                                                cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACttacc                                                                                                                          <  1:490373/230‑4 (MQ=255)                                                                                                                                                  tCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCttcttc                                                                                                                                                                     >  1:22024/1‑185 (MQ=255)                                                                                                                                                           cgcgcCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGaa                                                                                          <  1:392859/251‑1 (MQ=255)                                                                                                                                                                  tttCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                                                   <  1:598579/251‑1 (MQ=255)                                                                                                                                                                           ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                                                   <  1:199088/242‑1 (MQ=255)                                                                                                                                                                           ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATcc                                                                          <  1:298645/251‑1 (MQ=255)                                                                                                                                                                             cGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCgg                                                                        <  1:777076/251‑1 (MQ=255)                                                                                                                                                                                   ccTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAgg                                                                                            <  1:614403/225‑1 (MQ=255)                                                                                                                                                                                          cGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAAc                                                            <  1:246761/250‑1 (MQ=255)                                                                                                                                                                                             gCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTc                                                                                                                                                               <  1:872690/148‑1 (MQ=255)                                                                                                                                                                                             gCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCggg                                                                                                                  <  1:771244/193‑1 (MQ=255)                                                                                                                                                                                              cATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGt                                                       <  1:396053/251‑1 (MQ=255)                                                                                                                                                                                                tttCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTc                                                               <  1:676605/241‑1 (MQ=255)                                                                                                                                                                                                       gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                              >  1:615092/1‑251 (MQ=255)                                                                                                                                                                                                       gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                              >  1:511924/1‑251 (MQ=255)                                                                                                                                                                                                       gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                                              >  1:84541/1‑251 (MQ=255)                                                                                                                                                                                                        aGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                             >  1:872266/1‑251 (MQ=255)                                                                                                                                                                                                             gaagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTatcatc                                                                           <  1:804038/216‑1 (MQ=255)                                                                                                                                                                                                              aagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGttt                                        <  1:313335/250‑1 (MQ=255)                                                                                                                                                                                                                gaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCggg                                                                                                                  <  1:323530/174‑1 (MQ=255)                                                                                                                                                                                                                  cTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAata                                                                                                                                            >  1:753515/1‑146 (MQ=255)                                                                                                                                                                                                                  cTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTtctc                                     >  1:174844/1‑249 (MQ=255)                                                                                                                                                                                                                         tGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATc                             <  1:627571/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATc                                                                                                                                          >  1:805609/1‑131 (MQ=255)                                                                                                                                                                                                                                   aaGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGt                                                  >  1:392324/1‑219 (MQ=255)                                                                                                                                                                                                                                     ggAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                             <  1:760670/222‑1 (MQ=255)                                                                                                                                                                                                                                               accgtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTacac                                                                                                                               >  1:652347/5‑130 (MQ=255)                                                                                                                                                                                                                                               accgtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCa                                                                                                      <  1:364938/151‑1 (MQ=255)                                                                                                                                                                                                                                               accgtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                                             >  1:493451/5‑212 (MQ=255)                                                                                                                                                                                                                                                  gtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGt                                                       <  1:777079/198‑1 (MQ=255)                                                                                                                                                                                                                                                  gtataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCagag   >  1:662233/2‑251 (MQ=255)                                                                                                                                                                                                                                                   tataTTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAgg  <  1:746871/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATCGTTTGAGAAAACGCCCCTTTTCAGAGG  >  1/910547‑911039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑