Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	910,788	A→G	M70T (ATG→ACG)	unknown ←	Antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	910,788	0	A	G	100.0%	150.1 / NA	54	M70T (ATG→ACG)	unknown	Antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/31);  total (23/31)

GCTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATC  >  1/910546‑911012                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 gCTTAAAGTTCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatattg                                                                                                                                                                                                                           >  1:613198/1‑247 (MQ=255) gCTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatattg                                                                                                                                                                                                                           >  1:575340/1‑247 (MQ=255)  cTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatat                                                                                                                                                                                                                             <  1:482311/247‑2 (MQ=255)  cTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatat                                                                                                                                                                                                                             <  1:336205/247‑2 (MQ=255)  cTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGcc                                                                                                                                                                                                                         <  1:134821/251‑1 (MQ=255)        gTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCtt                                                                                                                                                                                                                   >  1:180539/1‑251 (MQ=255)                         gCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatat                                                                                                                                                                                                                             >  1:412816/1‑223 (MQ=255)                            ttttttAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGa                                                                                                                                                                                               >  1:520124/1‑251 (MQ=255)                                   aTGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGc                                                                                                                                                                                        <  1:633003/251‑1 (MQ=255)                                    tGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCg                                                                                                                                                                                       <  1:868643/251‑1 (MQ=255)                                        ccGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAAACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTa                                                                                                                                                                                   >  1:445081/1‑251 (MQ=255)                                          gATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTat                                                                                                                                                                                  >  1:496607/1‑250 (MQ=255)                                              aattaattTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCgtat                                                                                                                                                                                                                               >  1:869765/1‑201 (MQ=255)                                              aattaattTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGtatat                                                                                                                                                                                                                             >  1:405888/1‑202 (MQ=255)                                                           cccTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCaa                                                                                                                                                                >  1:882473/1‑251 (MQ=255)                                                                           gCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAc                                                                                                                                                 <  1:224717/250‑1 (MQ=255)                                                                            cTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAcc                                                                                                                                                <  1:556907/250‑1 (MQ=255)                                                                                           cAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                <  1:283375/251‑1 (MQ=255)                                                                                                            cGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATc                                                                                                               <  1:26603/251‑1 (MQ=255)                                                                                                               aaaTCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCtt                                                                                                             >  1:673810/1‑250 (MQ=255)                                                                                                                  tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGTCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                >  1:760461/1‑228 (MQ=255)                                                                                                                  tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCttcttc                                                                                                                                          <  1:828676/218‑1 (MQ=255)                                                                                                                  tCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAagt                                                                                                                                <  1:309282/228‑1 (MQ=255)                                                                                                                       taaGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTacac                                                                                                    <  1:759994/251‑1 (MQ=255)                                                                                                                                         cATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAAc                                                                                                                                                 <  1:457408/188‑1 (MQ=255)                                                                                                                                           tgtgAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCg                                                                                >  1:320440/1‑251 (MQ=255)                                                                                                                                                 cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGaa                                                                                                                                                                                              >  1:229456/1‑135 (MQ=255)                                                                                                                                                 cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCAcc                                                                                                         <  1:17590/220‑1 (MQ=255)                                                                                                                                                 cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTacac                                                                                                    >  1:780562/1‑225 (MQ=255)                                                                                                                                                 cATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACttacc                                                                                               <  1:490373/230‑4 (MQ=255)                                                                                                                                                   tCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCttcttc                                                                                                                                          >  1:22024/1‑185 (MQ=255)                                                                                                                                                            cgcgcCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGaa                                                               <  1:392859/251‑1 (MQ=255)                                                                                                                                                                   tttCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                        <  1:598579/251‑1 (MQ=255)                                                                                                                                                                            ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCg                                                        <  1:199088/242‑1 (MQ=255)                                                                                                                                                                            ttCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATcc                                               <  1:298645/251‑1 (MQ=255)                                                                                                                                                                              cGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCgg                                             <  1:777076/251‑1 (MQ=255)                                                                                                                                                                                    ccTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAgg                                                                 <  1:614403/225‑1 (MQ=255)                                                                                                                                                                                           cGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAAc                                 <  1:246761/250‑1 (MQ=255)                                                                                                                                                                                              gCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTc                                                                                                                                    <  1:872690/148‑1 (MQ=255)                                                                                                                                                                                              gCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCggg                                                                                       <  1:771244/193‑1 (MQ=255)                                                                                                                                                                                               cATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGt                            <  1:396053/251‑1 (MQ=255)                                                                                                                                                                                                 tttCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTc                                    <  1:676605/241‑1 (MQ=255)                                                                                                                                                                                                        gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                   >  1:511924/1‑251 (MQ=255)                                                                                                                                                                                                        gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                   >  1:84541/1‑251 (MQ=255)                                                                                                                                                                                                        gAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACa                   >  1:615092/1‑251 (MQ=255)                                                                                                                                                                                                         aGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                  >  1:872266/1‑251 (MQ=255)                                                                                                                                                                                                              gaagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTatcatc                                                <  1:804038/216‑1 (MQ=255)                                                                                                                                                                                                               aagaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGttt             <  1:313335/250‑1 (MQ=255)                                                                                                                                                                                                                 gaCTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCggg                                                                                       <  1:323530/174‑1 (MQ=255)                                                                                                                                                                                                                   cTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAata                                                                                                                 >  1:753515/1‑146 (MQ=255)                                                                                                                                                                                                                   cTTTTAGTGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTtctc          >  1:174844/1‑249 (MQ=255)                                                                                                                                                                                                                          tGAACGCAATAAGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATc  <  1:627571/250‑1 (MQ=255)                                                                                                                                                                                                                                    aaGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATc                                                                                                               >  1:805609/1‑131 (MQ=255)                                                                                                                                                                                                                                    aaGGAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGt                       >  1:392324/1‑219 (MQ=255)                                                                                                                                                                                                                                      ggAAAGCGGAACCGTATATTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTACCATTTCGGGAATATCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACAt                  <  1:760670/222‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 GCTTAAAGTGCGGTCAAATCGACCGCATTTTTTAATGAACCGATAAATTAATTTCGCGCCCTAATTTTTTAAAGGCTTTTTCAACAAAATCAATTTTGGATGGGTGGCGAAAATCTAATAAGCGATCGACTTGCGGCATGTGAACATCAAGACGGCGCGCCATTTCAGCTTTTCGCACACCTTGTGCGAGCATTTCATTGAGTAAGAAGACTTTTAGTGAACGCAATAAGGAAAGCGGAACCATATACTGCCCATCTTTTGGCTGGCTTGGCATTGGAATTGGGCGTTTATCATCAAAATAAAATTCAAGGGCGGTTTCTAAACCTTCTTCCGCTTCAAGTAGTGCGCTTTCAATATCTTCACCTACACTTGCCATTTCGGGAATGTCGGGGCAAGTGACAAGGAATGTACCGTTATCATCCGGGGTGATTTCAACAGGGTAGCGTAACATGGTTTCTCCTTAAATC  >  1/910546‑911012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑