Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	662	C→T	intergenic (+152/+58)	unknown → / ← unknown	Transposase, IS200 family/DNA‑directed RNA polymerase beta' subunit (EC 2.7.7.6)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	662	0	C	T	100.0%	210.1 / NA	79	intergenic (+152/+58)	unknown/unknown	Transposase, IS200 family/DNA‑directed RNA polymerase beta' subunit (EC 2.7.7.6)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/32);  total (47/32)

AAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCCTTATAGAGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCAAAACCGGT  >  1/463‑908                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       aaaGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTAc                                                                                                                                                                                                      <  1:291003/250‑1 (MQ=11)           atGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTAc                                                                                                                                                                                                      >  1:617262/1‑240 (MQ=11)              tAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         <  1:74886/250‑1 (MQ=37)              tAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         <  1:298880/250‑1 (MQ=37)                 gCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAGTCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGGCATAGAGGTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGGCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCggcg                                                                                                                                                                                     >  1:343786/1‑251 (MQ=11)                      gTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGAtt                                                                                                                                                                                >  1:86947/1‑251 (MQ=255)                      gTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGAtt                                                                                                                                                                                >  1:299459/1‑251 (MQ=255)                          ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         <  1:409492/238‑1 (MQ=37)                           gCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         >  1:886561/1‑237 (MQ=37)                           gCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGtctt                                                                                                                                                                           >  1:73772/1‑251 (MQ=255)                                       ggTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTAc                                                                                                                                                                                                      <  1:657211/212‑1 (MQ=11)                                       ggTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAATAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCa                                                                                                                                                                  >  1:429235/1‑248 (MQ=255)                                           gtgtAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTaa                                                                                                                                                           >  1:575243/1‑251 (MQ=255)                                           gtgtAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTaa                                                                                                                                                           >  1:874880/1‑251 (MQ=255)                                              tAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACa                                                                                                                                                         <  1:771822/250‑1 (MQ=255)                                              tAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACAt                                                                                                                                                        <  1:633869/251‑1 (MQ=255)                                                ggAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         >  1:670097/1‑216 (MQ=255)                                                   gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAAc                                                                                                                                                          <  1:95376/244‑1 (MQ=255)                                                   gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCgg                                                                                                                                                   >  1:322264/1‑251 (MQ=255)                                                        taAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAAc                                                                                                                                                          <  1:167692/239‑1 (MQ=255)                                                                 aGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTg                                                                                                                                          >  1:848992/1‑248 (MQ=255)                                                                  ggAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACg                                                                                                                                    >  1:846319/1‑251 (MQ=255)                                                                      aaGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATc                                                                                                                                <  1:501209/251‑1 (MQ=255)                                                                        ggAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCtt                                                                                                                              >  1:98651/1‑251 (MQ=255)                                                                              cATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAgg                                                                                                                        <  1:647540/251‑1 (MQ=255)                                                                                  aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         >  1:860568/1‑182 (MQ=255)                                                                                  aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGtcat                                                                                                                    >  1:283175/1‑251 (MQ=255)                                                                                        gATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCAc                                                                                                                 <  1:23357/248‑1 (MQ=255)                                                                                                     agaaagaaTATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAgg                                                                                                                        <  1:690926/228‑1 (MQ=255)                                                                                                      gaaagaaTATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTAc                                                                                                                                                                    <  1:215013/183‑1 (MQ=255)                                                                                                      gaaagaaTATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAAttt                                                                                                >  1:489321/1‑251 (MQ=255)                                                                                                       aaagaaTATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTT‑AACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATttc                                                                                               <  1:267835/250‑1 (MQ=255)                                                                                                             tatGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATttcttcttc                                                                                         <  1:79478/251‑1 (MQ=255)                                                                                                               tgtgGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGc                                                                                                                                         >  1:621219/1‑201 (MQ=255)                                                                                                                     ccccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccg                                                                                 >  1:29989/1‑251 (MQ=255)                                                                                                                       ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         <  1:287631/145‑1 (MQ=255)                                                                                                                       ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACg                                                                                                                >  1:628027/1‑218 (MQ=255)                                                                                                                       ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccgc                                                                                >  1:855852/1‑250 (MQ=255)                                                                                                                       ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccgc                                                                                >  1:726545/1‑250 (MQ=255)                                                                                                                        cTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCa                                                                                                                                                                  >  1:647587/1‑167 (MQ=255)                                                                                                                        cTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccgccg                                                                              >  1:251684/1‑251 (MQ=255)                                                                                                                        cTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccgccg                                                                              >  1:28163/1‑251 (MQ=255)                                                                                                                        cTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATccgccgccg                                                                              >  1:314374/1‑251 (MQ=255)                                                                                                                          ttAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGt                                                                                                                       >  1:194234/1‑208 (MQ=255)                                                                                                                              ggggTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGttt                                                                                                                                                             <  1:92433/166‑1 (MQ=255)                                                                                                                               gggTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATc                                                                        <  1:654091/250‑1 (MQ=255)                                                                                                                                   aGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGtcttc                                                                                                                                                                          >  1:877164/1‑148 (MQ=255)                                                                                                                                   aGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTAc                                                                    >  1:876145/1‑250 (MQ=255)                                                                                                                                     gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACg                                                                                                                <  1:94794/204‑1 (MQ=255)                                                                                                                                     gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAAttt                                                                                                <  1:106882/220‑1 (MQ=255)                                                                                                                                     gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCgg                                                                 >  1:335086/1‑251 (MQ=255)                                                                                                                                           gagTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCt                                                            >  1:887799/1‑250 (MQ=255)                                                                                                                                                   tCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCcg                                                   <  1:771318/251‑1 (MQ=255)                                                                                                                                                        gttgttGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGAt                                               <  1:314223/250‑1 (MQ=255)                                                                                                                                                                 tttCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCggg                                     <  1:401031/251‑1 (MQ=255)                                                                                                                                                                    cATATGCCCTTTGA‑‑AGGGCATGTGAGGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCa                                                                                                                                                                  <  1:866767/123‑1 (MQ=255)                                                                                                                                                                        tGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTAt                              <  1:840838/251‑1 (MQ=255)                                                                                                                                                                          cccTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTg                                                                                                                                       <  1:514842/144‑1 (MQ=255)                                                                                                                                                                          cccTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATc                                                     <  1:487337/226‑1 (MQ=255)                                                                                                                                                                          cccTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATgg                            >  1:18074/1‑251 (MQ=255)                                                                                                                                                                           ccTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATgg                            >  1:790904/1‑250 (MQ=255)                                                                                                                                                                            cTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         >  1:52692/1‑92 (MQ=255)                                                                                                                                                                            cTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGtcttcttc                                                                                                                                                                       <  1:102118/110‑1 (MQ=255)                                                                                                                                                                            cTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCg                          >  1:386817/1‑251 (MQ=255)                                                                                                                                                                            cTTTGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCg                          >  1:30211/1‑251 (MQ=255)                                                                                                                                                                              ttGA‑‑AGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTc                                                                                                                                                                                         <  1:734879/90‑1 (MQ=255)                                                                                                                                                                                        cATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGa                <  1:786890/251‑1 (MQ=255)                                                                                                                                                                                          tgtgAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATa              >  1:372050/1‑251 (MQ=255)                                                                                                                                                                                           gtgAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATa              >  1:167547/1‑250 (MQ=255)                                                                                                                                                                                           gtgAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATa              >  1:139539/1‑250 (MQ=255)                                                                                                                                                                                           gtgAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATa              >  1:594306/1‑250 (MQ=255)                                                                                                                                                                                             gAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACg            >  1:825191/1‑250 (MQ=255)                                                                                                                                                                                              aaGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGc           <  1:632912/250‑1 (MQ=255)                                                                                                                                                                                                gTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCt                                                                                                                                        >  1:589815/1‑123 (MQ=255)                                                                                                                                                                                                gTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTc                                                        >  1:192578/1‑203 (MQ=255)                                                                                                                                                                                                gTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCaaa        >  1:884277/1‑251 (MQ=255)                                                                                                                                                                                                      ggTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCAAAACCGGt  >  1:368540/1‑251 (MQ=255)                                                                                                                                                                                                      ggTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCAAAACCGGt  >  1:593522/1‑251 (MQ=255)                                                                                                                                                                                                      ggTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCAAAACCGGt  >  1:140292/1‑251 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       AAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGA‑‑AGGGCATGTGAAGTACTAGGTCCTTATAGAGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTACAGGAATCTTATTCCGCGTGATTGTCTTCTTCTACCATGTTTAACATTTCGGTTAAACTTGCTGACGCATCTTCGGAGGTCATCACGAATTCGGAAGCAATTTCTTCTTCATCCGCCGCCGATAATCTTACCGGCACCTCGTCATCCGCGATGGCTGTCGGGTTTTTATGGCGATTTCGGTGATACGCAAAACCGGT  >  1/463‑908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑