Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	888,904	(T)7→8	intergenic (+49/‑139)	unknown → / → unknown	UPF0339 protein YegP.N / UPF0339 protein YegP.C/PTS system, N‑acetylmannosamine‑specific IIC component / PTS system, N‑acetylmannosamine‑specific IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	888,897	1	.	T	100.0%	80.0 / NA	29	intergenic (+42/‑146)	unknown/unknown	UPF0339 protein YegP.N / UPF0339 protein YegP.C/PTS system, N‑acetylmannosamine‑specific IIC component / PTS system, N‑acetylmannosamine‑specific IIB component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (21/8);  total (21/8)

ACGGAGTGGATGCAAAAAACTTTGAATTTCGTACTACAAAAAATGATCAGCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGA‑TTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTGCCCGCTGCCGGTTTGCTGTTAGGGATTGGCGG  >  1/888665‑889131                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           aCGGAGTGGATGCAAAAAACTTTGAATTTCGTACCACAAAAAATGATCAGCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATg                                                                                                                                                                                                                           <  1:589763/251‑1 (MQ=255)                             tCGTACCACAAAAAATGATCAGCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGt                                                                                                                                                                                               >  1:384318/1‑251 (MQ=255)                                           aTGATCAGCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAg                                                                                                                                                                                        >  1:406627/1‑244 (MQ=255)                                                  gCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATATAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCaaaaa                                                                                                                                                                          >  1:310405/1‑251 (MQ=255)                                                            gTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAata                                                                                                                                                                >  1:588768/1‑251 (MQ=255)                                                                                     aaaTCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAat                                                                                                                                        <  1:675857/250‑1 (MQ=255)                                                                                                                         ctgcACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAgagg                                                                                                    <  1:508684/250‑1 (MQ=255)                                                                                                                                aaaaGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGA‑ttttttt                                                                                                                                                                                                                                     >  1:766321/1‑113 (MQ=255)                                                                                                                                    ggTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATg                                                                                        >  1:617068/1‑251 (MQ=255)                                                                                                                                              ccGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAaacaa                                                                               >  1:11647/1‑250 (MQ=255)                                                                                                                                                gTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACtt                                                                            >  1:262006/1‑251 (MQ=255)                                                                                                                                                     gAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAgagg                                                                                                    >  1:575495/1‑222 (MQ=255)                                                                                                                                                                                    cTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGc                                         >  1:651296/1‑250 (MQ=255)                                                                                                                                                                                     ttACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGcc                                               <  1:487249/243‑1 (MQ=255)                                                                                                                                                                                        ccGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGa                                                                                                                                                          >  1:235193/1‑133 (MQ=255)                                                                                                                                                                                        ccGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCAttt                                    >  1:889815/1‑251 (MQ=255)                                                                                                                                                                                          gTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTg                                  >  1:242558/1‑251 (MQ=255)                                                                                                                                                                                          gTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAACGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGt                                                   <  1:435981/234‑1 (MQ=255)                                                                                                                                                                                            ataataTACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAat                                                                                                                                        <  1:90163/147‑1 (MQ=255)                                                                                                                                                                                               atatACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGaaa                                                              >  1:79465/1‑218 (MQ=255)                                                                                                                                                                                                  taCATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAata                                                                                                                                                                >  1:91578/1‑117 (MQ=255)                                                                                                                                                                                                   aCATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAg                                                                                                                                                                                        >  1:812543/1‑92 (MQ=255)                                                                                                                                                                                                   aCATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAgagg                                                                                                    >  1:326809/1‑176 (MQ=255)                                                                                                                                                                                                   aCATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAAt                                                                                             >  1:702499/1‑183 (MQ=255)                                                                                                                                                                                                    cATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAgagg                                                                                                    >  1:372909/1‑175 (MQ=255)                                                                                                                                                                                                            aGATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTGCCCGCTGCCGGTTTg                   >  1:107335/1‑248 (MQ=255)                                                                                                                                                                                                             gATTGGGATTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAg                                                                                                                                                                                        <  1:479778/82‑1 (MQ=255)                                                                                                                                                                                                                    aTTGTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAatat                                                                                                                                                                                  >  1:129725/1‑81 (MQ=255)                                                                                                                                                                                                                       gTCGGCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTGCCCGCTGCCGGTTTGCTGTTAGGGATTgg     >  1:439374/1‑251 (MQ=255)                                                                                                                                                                                                                           gCACTTCGGTGCCGATTTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTGCCCGCTGCCGGTTTGCTGTTAGGGATTGGCgg  <  1:162883/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           ACGGAGTGGATGCAAAAAACTTTGAATTTCGTACTACAAAAAATGATCAGCCTTATTTCGTATTAAAAGCGGCAAATCATCAGGAAATCGGTCGCAGCCAATATTATTCTTCGCAAGCTGCTGCACAAAAAGGTGTAGAGTCCGTAATGAACAACGCTTCCAGCGAAGATATTCGGGATCTTACCGTATAATATACATAAAATAGATTGGGATTGTCGGCACTTCGGTGCCGA‑TTTTTTTGCTTAAAATGCGATCTCAGTCATGTTTTTTCGTCAAGTATTTAAGTAATATTCCAAAAAATAGTTAATATTTAGAAATGTAACTCCATAAAATATTTCAAGATAAATCATAAATTGCGTCGTGAAGAGGAGAGAATTTATGTTTAAACAACTTCAACAAGTCGGAAAGGCATTTATGTTGCCTATTGCCATTTTGCCCGCTGCCGGTTTGCTGTTAGGGATTGGCGG  >  1/888665‑889131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑