Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	617,535	+T	intergenic (‑63/‑152)	unknown ← / → unknown	Energy‑dependent translational throttle protein EttA/putative exported protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	617,534	1	.	T	100.0%	132.7 / NA	50	intergenic (‑62/‑153)	unknown/unknown	Energy‑dependent translational throttle protein EttA/putative exported protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (22/28);  total (22/28)

CTTTATCGATGCCTGCCATAATGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGA‑TGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCGTGTGAAGAAAAATACTAAATTAAGTTT  >  1/617298‑617763                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      cTTTATCGATGCCTGCCATAATGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAAt                                                                                                                                                                                                                          >  1:727053/1‑251 (MQ=255)           gcctgccATAATGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATc                                                                                                                                                                                                                    >  1:467299/1‑247 (MQ=255)               gccATAATGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCgggg                                                                                                                                                                                                            >  1:386822/1‑251 (MQ=255)                    aaTGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCa                                                                                                                                                                                                       >  1:855053/1‑251 (MQ=255)                                  tGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGt                                                                                                                                                                                          <  1:69273/250‑1 (MQ=255)                                   ggATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGttt                                                                                                                                                                                        >  1:560826/1‑251 (MQ=255)                                                                cGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCGTCTTTGTGCTAATCATTCGCGGAAAAc                                                                                                                                                           <  1:836638/251‑1 (MQ=255)                                                                     aTTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGata                                                                                                                                                      <  1:70259/251‑1 (MQ=255)                                                                           gcgcCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATaaaa                                                                                                                                                 <  1:581079/250‑1 (MQ=255)                                                                                 gggAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACct                                                                                                                                           >  1:524385/1‑250 (MQ=255)                                                                                    aaaaaGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTcg                                                                                                                                       <  1:557693/251‑1 (MQ=255)                                                                                           tGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCgaggag                                                                                                                                <  1:134631/251‑1 (MQ=255)                                                                                              gTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATACTTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCAt                                                                                                                                                                                                      >  1:747605/1‑178 (MQ=255)                                                                                                 aaaTATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAg                                                                                                                          <  1:225734/251‑1 (MQ=255)                                                                                                   atatTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGa                                                                                                                        <  1:341726/251‑1 (MQ=255)                                                                                                    tatTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGaa                                                                                                                       <  1:231406/251‑1 (MQ=255)                                                                                                        tttCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATgg                                                                                                                                                                                                                             >  1:701577/1‑145 (MQ=255)                                                                                                         ttCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAagtag                                                                                                                  <  1:704312/251‑1 (MQ=255)                                                                                                          tCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAagtagt                                                                                                                 <  1:398420/251‑1 (MQ=255)                                                                                                               atatGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGc                                                                                                            <  1:869325/251‑1 (MQ=255)                                                                                                                                 actactTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGAttattttat                                                                                          <  1:8461/251‑1 (MQ=255)                                                                                                                                         gCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTcg                                                                                                                                                                   <  1:849330/170‑1 (MQ=255)                                                                                                                                             aCTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTa                                                                              >  1:738986/1‑251 (MQ=255)                                                                                                                                              cTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATtattttattt                                                                                        <  1:288669/240‑1 (MQ=255)                                                                                                                                                  gTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGaa                                                                          <  1:89164/250‑1 (MQ=255)                                                                                                                                                  gTGCATCGTATAAACGAATTGGATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCg                                                                                                                                                                                                               >  1:348787/1‑117 (MQ=255)                                                                                                                                                           ataAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCaa                                                                <  1:98361/251‑1 (MQ=255)                                                                                                                                                                cGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAAc                                                                                                                                                           <  1:424725/155‑1 (MQ=255)                                                                                                                                                                  aaTTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGata                                                                                                                                                      >  1:3185/1‑158 (MQ=255)                                                                                                                                                                  aaTTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTg                                                         <  1:530820/251‑1 (MQ=255)                                                                                                                                                                      gTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGCGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAAtt                                                                                                                                                                                                                         >  1:90388/1‑87 (MQ=255)                                                                                                                                                                             cATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGa                                                                                                                        >  1:629482/1‑177 (MQ=255)                                                                                                                                                                               tAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAAt                                                                                                                                                                                                                     >  1:707994/1‑82 (MQ=255)                                                                                                                                                                               tAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTc                                                                                                                                                                                                    >  1:739471/1‑99 (MQ=255)                                                                                                                                                                               tAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAAcc                                                                                                                                            >  1:401102/1‑155 (MQ=255)                                                                                                                                                                               tAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGATAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATg                                            >  1:452637/1‑251 (MQ=255)                                                                                                                                                                                aaTTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGc                                           <  1:560487/251‑1 (MQ=255)                                                                                                                                                                                 attattCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAAc                                                                                  >  1:168730/1‑211 (MQ=255)                                                                                                                                                                                      tctcTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGa                                     <  1:328676/251‑1 (MQ=255)                                                                                                                                                                                       ctctGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGAt                                    >  1:670669/1‑251 (MQ=255)                                                                                                                                                                                         ctGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGaa                                                                                                                       >  1:257186/1‑166 (MQ=255)                                                                                                                                                                                           gggTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTa                                                                              <  1:554642/205‑1 (MQ=255)                                                                                                                                                                                           gggTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCGCGCGAGGAGAGGTAGGAAagt                                                                                                                    <  1:123169/167‑1 (MQ=255)                                                                                                                                                                                                aaGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCgtg                           <  1:137685/251‑1 (MQ=255)                                                                                                                                                                                                aaGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCgtg                           <  1:636783/251‑1 (MQ=255)                                                                                                                                                                                                   ggAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGa                                                                                                                        >  1:834052/1‑155 (MQ=255)                                                                                                                                                                                                       aaGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCGTGTgaagaa                    <  1:5744/251‑1 (MQ=255)                                                                                                                                                                                                         gTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATc                                                                                                                                                                                    <  1:858818/89‑1 (MQ=255)                                                                                                                                                                                                                  gAATTCAAGGCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCggggg                                                                                                                                                                                                           >  1:440021/1‑57 (MQ=255)                                                                                                                                                                                                                           gCGTTTTTTCGGCACCTGATTGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCGTGTGAAGAAAAATACTAAATTAAGttt  <  1:753422/249‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      CTTTATCGATGCCTGCCATAATGCGAAGCAGGGTGGATTTACCGGCACCGTTTAAGCCAAGTACGCCGATTTTGGCGCCCGGGAAAAAGCTGAGTGAAATATTTTTCAGAATATGACGTTTCGGCGGAACTACTTTGCCTACTCGGTGCATCGTATAAACGAATTGTATTGACATAATTATTCTCTGGGTTAAGGGAAAAGTGCGGTCGGAATTCAAGGCGTTTTTTCGGCACCTGA‑TGACCGATGGAATTTAATCATTCGGGGGTTCATTCTACTTGATGTTTCATCTTTGTGCTAATCATTCGCGGAAAACAGATATAAAAAAACCTCTCGCGAGGAGAGGTAGGAAAGTAGTTTAGCTATTTATGATTATTTTATTTAGGAACTTTATGAATATCAAGCAATCACTTGACGCGATTGAATGCTATAGATTTTTTCGTGTGAAGAAAAATACTAAATTAAGTTT  >  1/617298‑617763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑