Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,571,051	+A	coding (337/357 nt)	unknown →	UPF0225 protein YchJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,571,051	1	.	A	100.0%	206.2 / NA	76	L113M (CTG→ATG)	unknown	UPF0225 protein YchJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (40/36);  total (40/36)

CTACCGAGCAGCTCATGCGCTCGCGCTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATC‑TGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCACCATGCTCGCCATT  >  1/1570812‑1571288                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              cTACCGAGCAGCTCATGCGCTCGCGCTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCg                                                                                                                                                                                                                                     >  1:203527/1‑251 (MQ=255) cTACCGAGCAGCTCATGCGCTCGCGCTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCg                                                                                                                                                                                                                                     >  1:616064/1‑251 (MQ=255)      gAGCAGCTCATGCGCTCGCGCTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGAtttt                                                                                                                                                                                                                                >  1:673711/1‑251 (MQ=255)                       gcgcTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAgg                                                                                                                                                                                                                >  1:856060/1‑250 (MQ=255)                        cgcTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCg                                                                                                                                                                                                              >  1:357456/1‑251 (MQ=255)                             tGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGt                                                                                                                                                                                                         <  1:178102/251‑1 (MQ=255)                             tGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGt                                                                                                                                                                                                         <  1:775281/251‑1 (MQ=255)                                 gcgTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTAttt                                                                                                                                                                                                     >  1:874722/1‑251 (MQ=255)                                       gtgtTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGa                                                                                                                                                                                               >  1:456417/1‑251 (MQ=255)                                       gtgtTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGa                                                                                                                                                                                               >  1:421275/1‑251 (MQ=255)                                         gtTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGAt                                                                                                                                                                                              >  1:68693/1‑250 (MQ=255)                                                     gCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTtg                                                                                                                                                                                                   <  1:832201/233‑1 (MQ=255)                                                     gCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCg                                                                                                                                                                                  >  1:97173/1‑250 (MQ=255)                                                     gCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCg                                                                                                                                                                                  >  1:851550/1‑250 (MQ=255)                                                       cTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTa                                                                                                                                                                               <  1:415107/251‑1 (MQ=255)                                                        ttATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTAc                                                                                                                                                                              <  1:758328/251‑1 (MQ=255)                                                          atatTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTAt                                                                                                                                                                                                       <  1:342503/224‑1 (MQ=255)                                                          atatTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACg                                                                                                                                                                                        <  1:287548/239‑1 (MQ=255)                                                          atatTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCg                                                                                                                                                                                  >  1:817505/1‑245 (MQ=255)                                                                 ggAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGa                                                                                                                                                                     >  1:540187/1‑251 (MQ=255)                                                                      ccaccGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAg                                                                                                                                                                <  1:394467/251‑1 (MQ=255)                                                                              ccGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCat                                                                                                                                                        <  1:350750/251‑1 (MQ=255)                                                                                   ccAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCg                                                                                                                                                   <  1:129945/251‑1 (MQ=255)                                                                                               gctgAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCaaaa                                                                                                                                       >  1:143675/1‑251 (MQ=255)                                                                                                     cGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTa                                                                                                                                 >  1:766388/1‑251 (MQ=255)                                                                                                           ggCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCAtt                                                                                                                           >  1:349518/1‑251 (MQ=255)                                                                                                               aTTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTCTTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAAc                                                                                                                                                                  >  1:439698/1‑208 (MQ=255)                                                                                                               aTTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCAttgttg                                                                                                                       >  1:361782/1‑251 (MQ=255)                                                                                                               aTTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCAttgttg                                                                                                                       >  1:63577/1‑251 (MQ=255)                                                                                                                    aaCCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCggag                                                                                                                  >  1:551822/1‑251 (MQ=255)                                                                                                                    aaCCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCggag                                                                                                                  <  1:490936/251‑1 (MQ=255)                                                                                                                     aCCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCggagg                                                                                                                 <  1:702965/251‑1 (MQ=255)                                                                                                                            cGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATGTTTa                                                                                                          <  1:575075/251‑1 (MQ=255)                                                                                                                                  aTACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTAtgattg                                                                                                    <  1:484090/251‑1 (MQ=255)                                                                                                                                     cGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGAc                                                                                                                                                                          <  1:380920/178‑1 (MQ=255)                                                                                                                                       cAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTa                                                                                               >  1:226277/1‑251 (MQ=255)                                                                                                                                           ggcttggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTg                                                                                                                                                                                                                     >  1:393116/1‑129 (MQ=255)                                                                                                                                           ggcttggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCgg                                                                                                                    <  1:786061/226‑1 (MQ=255)                                                                                                                                           ggcttggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGCGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTaaaaa                                                                                           >  1:405467/1‑251 (MQ=255)                                                                                                                                             cttggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAAcc                                                                                         <  1:723208/251‑1 (MQ=255)                                                                                                                                             cttggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAAcc                                                                                         <  1:571458/251‑1 (MQ=255)                                                                                                                                                ggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATcatgaa                                                                                                                                                                                                                                           <  1:167795/102‑6 (MQ=255)                                                                                                                                                ggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGAcc                                                                                                                                                                         <  1:658398/168‑1 (MQ=255)                                                                                                                                                ggcttACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCg                                                                                                                                                   >  1:675502/1‑190 (MQ=255)                                                                                                                                                        gATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGaa                                                                                                                                                                    >  1:509325/1‑165 (MQ=255)                                                                                                                                                         aTTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCat                                                                                                                                                        <  1:672114/176‑1 (MQ=255)                                                                                                                                                          ttCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCgttgt                                                                            <  1:191478/251‑1 (MQ=255)                                                                                                                                                              gAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTtgt                                                                                                                                                                                                  >  1:183632/1‑129 (MQ=255)                                                                                                                                                                 tgcGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCggag                                                                                                                  >  1:696009/3‑206 (MQ=255)                                                                                                                                                                 tACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCggag                                                                                                                  >  1:696044/1‑206 (MQ=255)                                                                                                                                                                 tACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCa                                                                     <  1:589101/251‑1 (MQ=255)                                                                                                                                                                   cGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCa                                                                     <  1:657688/249‑1 (MQ=255)                                                                                                                                                                        aCCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCGAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCgttgtt                                                                           >  1:257890/1‑238 (MQ=255)                                                                                                                                                                                 aaGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAAt                                                     >  1:337682/1‑251 (MQ=255)                                                                                                                                                                                 aaGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAAt                                                     <  1:351186/251‑1 (MQ=255)                                                                                                                                                                                   ggTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCAttgttg                                                                                                                       <  1:319994/183‑1 (MQ=255)                                                                                                                                                                                       cAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACt                                                <  1:262510/250‑1 (MQ=255)                                                                                                                                                                                           gTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACg                                            >  1:143843/1‑250 (MQ=255)                                                                                                                                                                                                     aTTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATc                                                                                                 <  1:442450/187‑1 (MQ=255)                                                                                                                                                                                                     aTTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCgg                                 >  1:385115/1‑251 (MQ=255)                                                                                                                                                                                                             ccATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAGAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCt                                                                                                                                                             >  1:654767/1‑119 (MQ=255)                                                                                                                                                                                                             ccATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGa                         >  1:550153/1‑251 (MQ=255)                                                                                                                                                                                                               aTTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAAc                                                                                          <  1:105020/184‑1 (MQ=255)                                                                                                                                                                                                                 ttttCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCa                                                                     >  1:312893/1‑203 (MQ=255)                                                                                                                                                                                                                  tttCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATTCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTAc                    <  1:425987/251‑1 (MQ=255)                                                                                                                                                                                                                  tttCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTAc                    <  1:274865/251‑1 (MQ=255)                                                                                                                                                                                                                    tCAGGGCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTAACGTACAg                  <  1:263454/251‑1 (MQ=255)                                                                                                                                                                                                                        ggCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCAc               <  1:391852/250‑1 (MQ=255)                                                                                                                                                                                                                        ggCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCAc               <  1:292074/250‑1 (MQ=255)                                                                                                                                                                                                                         gCGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTAt                                                                                                                                                                                                       >  1:848173/1‑65 (MQ=255)                                                                                                                                                                                                                          cGAAGAAGGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCgg                                                                                                                    <  1:723553/147‑1 (MQ=255)                                                                                                                                                                                                                            aagaagGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCACCATGc          <  1:876789/251‑1 (MQ=255)                                                                                                                                                                                                                              gaagGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCAc               >  1:799004/1‑244 (MQ=255)                                                                                                                                                                                                                               aagGTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCACCATGCTc        <  1:70978/250‑1 (MQ=255)                                                                                                                                                                                                                                 ggTGAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTa                                                                                                          >  1:754722/1‑150 (MQ=255)                                                                                                                                                                                                                                    gAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCat                                                                                                                                                        >  1:271599/1‑101 (MQ=255)                                                                                                                                                                                                                                    gAGCAGGCGCATCATGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCACCATGCTCGCCAtt  >  1:333638/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              CTACCGAGCAGCTCATGCGCTCGCGCTATGCCGCGTATGTGTTAAAAAATGTGCCTTATATTGTGGAAACCACCGTGCCGAACCAACAAAAGCTGCTGAACGTGCAGGCAATTCAAACCTGGGCGGAAAATACGCAATGGCTTGGCTTACAGATTCTGAATACGGAAACCCTGACGAAGGTGCAAAGTGCGGTGGAATTTAACGCCATTTTTCAGGGCGAAGAAGGTGAGCAGGCGCATC‑TGAACGTTCGATTTTTGTGAAAATTGACAGGCGTTGGTATTTTGTGGATCCGACGGTGCCGCTACCGACCATGAAACAGCCTTGCATATGCGATTCCGGCAAAAAATTTAAGCATTGTTGCGGAGGATTTTTATGATTGATCTAAAAACCTTTTGTGCGTTGTTTTGGCAACGTTTTAATCAAAATAAACTCACGCAAGCCGCCGGTTATCTGACGTACAGCACCATGCTCGCCATT  >  1/1570812‑1571288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑