Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,974	+G	intergenic (‑558/‑191)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,974	1	.	G	100.0%	43.2 / NA	17	intergenic (‑558/‑191)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (9/8);  total (9/8)

AAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTAAATAAATGACATTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACCTTAATTACGAAAGCA  >  1/1477745‑1478191                                                                                                                                                                                                                                         |                                                                                                                                                                                                                           taaaTATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAatacg                                                                                                                                                                                                         <  1:512730/250‑2 (MQ=255)          ttttttAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAc                                                                                                                                                                                                <  1:895687/250‑1 (MQ=255)                                                                       ttCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatataccggt                                                                                                                                    >  1:232544/1‑243 (MQ=255)                                                                                agaaaaaaGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac                                                                                                                                        <  1:56866/234‑4 (MQ=255)                                                                                                 cgcgATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCttatta                                                                                                        <  1:354355/251‑1 (MQ=255)                                                                                                                           tttaagtgtttttGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAcat                                                                                                                                                                                              <  1:385564/133‑3 (MQ=255)                                                                                                                                                aGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAcat                                                                                                                                                                                              >  1:180167/1‑118 (MQ=255)                                                                                                                                                           gTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATtta                                                                                                                                                                                    >  1:146277/1‑119 (MQ=255)                                                                                                                                                                            tCAATAGAGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGAc                              <  1:225005/251‑1 (MQ=255)                                                                                                                                                                                 agagAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAcat                                                                                                                                                                                              >  1:503919/4‑85 (MQ=255)                                                                                                                                                                                       aGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACtatatac                                                                                                                                        >  1:268495/1‑132 (MQ=255)                                                                                                                                                                                        gTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAatacgta                                                                                                                                                                                                       >  1:150601/1‑68 (MQ=255)                                                                                                                                                                                                        tACAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACTTTAACTACTAAAGCa  >  1:484344/1‑251 (MQ=255)                                                                                                                                                                                                         aCAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATAc                                                                                                                                                                                                >  1:318244/1‑61 (MQ=255)                                                                                                                                                                                                          cAATAACCCTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAg                                                                                                               <  1:151415/140‑1 (MQ=255)                                                                                                                                                                                                                cccTTAACATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAg                                <  1:497732/213‑1 (MQ=255)                                                                                                                                                                                                                     aaCATACTGTTAATTAAAAAGTTTTCGCAATCACAATACGTACATATACATAGTGAATTTATTATTCTAAAAAAACTTGCATTTTAATTTTTCAGGACTAT‑ATACCGGTCGTTTATTTAATTCCACAAGCTTATTAAATAAATGACACTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGAc                              >  1:199993/1‑210 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                           AAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCATTTACAATAACCCTTAACATACTGTTAATTAAAAA‑TTTTCGCAATCACAATACATACATATACGTAGTGAATTTATTATTCT‑AAAAAACTTGCATTTTAATTTTTCAGGACTATAATGCCACTCGTTTATTTAATTCCACAAGATTATTAAATAAATGACATTAAGTTCAAACTCAAGGATTCTTGAATTTTATTTTCTATAAACAACAAAGGAGCATTAAGACAATGTTAAATACCTTAATTACGAAAGCA  >  1/1477745‑1478191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑