Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	203,792	+T	coding (1080/1098 nt)	unknown →	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	203,792	1	.	T	100.0%	137.4 / NA	51	G360G (GGA→GGT)	unknown	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (16/35);  total (16/35)

TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTAT  >  1/203617‑204034                                                                                                                                                                                 |                                                                                                                                                                                                                                                   caaattgctcgctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGTCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCg                                                                                                                                                                          <  1:446493/230‑1 (MQ=255)          cgctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTg                                                                                                                                                                                                                                               <  1:193484/161‑1 (MQ=255)            ctccgctacagggCAATTGCAATTGTCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACTTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCACTGTTGCTGACCATGGCTGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                               >  1:464602/11‑251 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                               >  1:764158/11‑251 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                               <  1:205039/241‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                                                                             <  1:315677/211‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGtt                                                                                                                                                                                 <  1:861/223‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTattatt                                                                                                                                                          >  1:30640/6‑251 (MQ=255)                         caattgcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                   <  1:692873/250‑1 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAatat                                                                                                                                                                                                                              >  1:274086/1‑170 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGagc                                                                                                                                                                                                                          >  1:110978/1‑174 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGt                                                                                                                                                                                                          <  1:509933/190‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                  <  1:507999/251‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                  <  1:328278/251‑1 (MQ=255)                                                          aaaaCTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCattat                                                                                                                 <  1:58209/251‑1 (MQ=255)                                                                     aTTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                                                                             >  1:59172/1‑164 (MQ=255)                                                                      tttCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCaa                                                                                                     <  1:740735/251‑1 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                  >  1:458119/1‑219 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTaa                                                                                                            >  1:46054/1‑241 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTTCAACgg                                                                                                  >  1:582164/1‑251 (MQ=255)                                                                           tcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGAc                                                                                                <  1:708083/251‑1 (MQ=255)                                                                                    aTGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATc                                                                                                          >  1:612092/1‑232 (MQ=255)                                                                                    aTGGCGTCCACTTTAGAAGAATTCTCGAATGCTGCCGGCTCGGCAAGTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACATCTGCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAAtt                                                                                       <  1:585574/251‑1 (MQ=255)                                                                                     tGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAAttt                                                                                      <  1:705535/251‑1 (MQ=255)                                                                                                    aaaaaTTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATATTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCaaaaa                                                                       <  1:55149/251‑1 (MQ=255)                                                                                                           cccGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTaaaa                                                                                                                           <  1:590240/192‑1 (MQ=255)                                                                                                               aaTGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAg                                                             <  1:842539/250‑1 (MQ=255)                                                                                                                 tGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTAc                                                          <  1:655250/251‑1 (MQ=255)                                                                                                                            cGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACTGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGAGGTTTGCTATTATTTTTTAAc                                                                                                                                                   >  1:673694/1‑151 (MQ=255)                                                                                                                                aaaTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAATGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTtata                                           <  1:43259/251‑1 (MQ=255)                                                                                                                                  aTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAat                                                                                                                                                                                                                                >  1:93618/1‑68 (MQ=255)                                                                                                                                  aTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATa                                                                                           >  1:8348/1‑201 (MQ=255)                                                                                                                                  aTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAAttt                                                                                      >  1:474187/1‑206 (MQ=255)                                                                                                                                   ttCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGt                                                            >  1:76078/1‑231 (MQ=255)                                                                                                                                         ggTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                               <  1:244868/126‑1 (MQ=255)                                                                                                                                         ggTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATc                                                                                                          <  1:190305/179‑1 (MQ=255)                                                                                                                                           tCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTAc                                <  1:50599/251‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                   <  1:547594/133‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAg                                                                                                                         <  1:534670/159‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATa                                                                                           <  1:378585/189‑1 (MQ=255)                                                                                                                                                 gtgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                  <  1:294532/147‑1 (MQ=255)                                                                                                                                                 gtgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGt                                                            <  1:415463/217‑1 (MQ=255)                                                                                                                                                  tgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCg                         <  1:201927/251‑1 (MQ=255)                                                                                                                                                   gtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATg                                         <  1:336228/234‑1 (MQ=255)                                                                                                                                                        tttCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                   <  1:368503/123‑1 (MQ=255)                                                                                                                                                               cccGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTcc            <  1:848675/250‑1 (MQ=255)                                                                                                                                                               aCCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTcc            <  1:662919/251‑1 (MQ=255)                                                                                                                                                                        tGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCt                                                                                 <  1:183838/173‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACgg                                                                                                  <  1:110603/155‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGcc                       <  1:721439/230‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTAt  >  1:360717/1‑251 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                                                                   TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTAT  >  1/203617‑204034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑