Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 146,302 (A)8→9 intergenic (‑49/+190) unknown ← / ← unknown Hypoxanthine‑guanine phosphoribosyltransferase (EC 2.4.2.8)/TldE protein, part of TldE/TldD proteolytic complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1146,2941.A99.2% 333.5 / ‑3.5 119intergenic (‑41/+198)unknown/unknownHypoxanthine‑guanine phosphoribosyltransferase (EC 2.4.2.8)/TldE protein, part of TldE/TldD proteolytic complex
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (45/73);  total (46/73)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AGGTCGTCATAAATTCAATTTCTACCGGCAAATGCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAC‑‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATTATTATCATTTACAAAAAATATTTTAATTACCGGAAATTTTCATATTTTCCAAGAGAATAGAAC  >  1/146046‑146532
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               
aGGTCGTCATAAATTCAATTTCTACCGGCAAATGCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAC‑‑a                                                                                                                                                                                                                                               <  1:174075/250‑1 (MQ=255)
                        ccGGCAAATGCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACAAAAAAAAAAGACTGAAGCTTAAACt                                                                                                                                                                                                                        <  1:499158/251‑1 (MQ=255)
                               aTGCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCtt                                                                                                                                                                                                                >  1:744797/1‑251 (MQ=255)
                                 gCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCtttt                                                                                                                                                                                                              >  1:56365/1‑251 (MQ=255)
                                      tCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAAt                                                                                                                                                                                                         <  1:629258/251‑1 (MQ=255)
                                       cgcgCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATa                                                                                                                                                                                                        <  1:7890/251‑1 (MQ=255)
                                           cACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATAcc                                                                                                                                                                                                      >  1:538666/1‑249 (MQ=255)
                                              cAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAAc                                                                                                                                                                                                 <  1:834207/251‑1 (MQ=255)
                                               aaaTCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACg                                                                                                                                                                                                <  1:129430/251‑1 (MQ=255)
                                                aaTCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGa                                                                                                                                                                                               <  1:781854/251‑1 (MQ=255)
                                                aaTCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGa                                                                                                                                                                                               <  1:719482/251‑1 (MQ=255)
                                                     gCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAc                                                                                                                                                                                                                                                  >  1:142395/1‑196 (MQ=255)
                                                          gAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACAAAAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACttt                                                                                                                                                                                      >  1:605144/1‑251 (MQ=255)
                                                          gAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTAc                                                                                                                                                                                         >  1:52377/1‑247 (MQ=255)
                                                          gAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACttt                                                                                                                                                                                      >  1:421844/1‑250 (MQ=255)
                                                            aCATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGGGTTTTTTCATTTAGGGTTCCCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTAAAGCTTAAATTTCAGTCTTTTAAATTACCCTAACGAATTTACTTtct                                                                                                                                                                                    >  1:240330/1‑250 (MQ=255)
                                                             cATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTtcta                                                                                                                                                                                   >  1:101937/1‑250 (MQ=255)
                                                             cATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAC‑‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCaaa                                                                                                                                                                                                          >  1:105912/1‑226 (MQ=255)
                                                               tAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACt                                                                                                                                                                                                                        <  1:249360/211‑1 (MQ=255)
                                                               tAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTtctactc                                                                                                                                                                                <  1:694976/251‑1 (MQ=255)
                                                               tAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTtctact                                                                                                                                                                                 >  1:716856/1‑250 (MQ=255)
                                                                     aaCCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTg                                                                                                                                                                          <  1:582832/251‑1 (MQ=255)
                                                                          cgcAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTgg                                                                                                                                                                     <  1:513248/251‑1 (MQ=255)
                                                                          cgcAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTgg                                                                                                                                                                     >  1:242146/1‑251 (MQ=255)
                                                                            cAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCa                                                                                                                                                                                                            >  1:155458/1‑210 (MQ=255)
                                                                            cAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGa                                                                                                                                                                                               <  1:348624/223‑1 (MQ=255)
                                                                            cAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGttt                                                                                                                                                                       <  1:852773/247‑1 (MQ=255)
                                                                            cAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAg                                                                                                                                                                   >  1:85331/1‑251 (MQ=255)
                                                                             aaTAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGt                                                                                                                                                                  <  1:28136/251‑1 (MQ=255)
                                                                             aaTAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGt                                                                                                                                                                  <  1:85087/251‑1 (MQ=255)
                                                                             aaTAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGt                                                                                                                                                                  <  1:728338/251‑1 (MQ=255)
                                                                               tAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTAc                                                                                                                                                                <  1:2209/251‑1 (MQ=255)
                                                                               tAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTAc                                                                                                                                                                <  1:248575/251‑1 (MQ=255)
                                                                                   ccTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATg                                                                                                                                                            <  1:737137/251‑1 (MQ=255)
                                                                                    cTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           <  1:466571/251‑1 (MQ=255)
                                                                                    cTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           <  1:603327/251‑1 (MQ=255)
                                                                                      aCCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAg                                                                                                                                                         <  1:207426/251‑1 (MQ=255)
                                                                                      aCCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAg                                                                                                                                                         >  1:6606/1‑251 (MQ=255)
                                                                                          caACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATAc                                                                                                                                                                                                       <  1:900742/201‑1 (MQ=255)
                                                                                          caACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACtt                                                                                                                                                     >  1:347205/1‑251 (MQ=255)
                                                                                          caACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACtt                                                                                                                                                     >  1:82342/1‑251 (MQ=255)
                                                                                            aCCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGAGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTAc                                                                                                                                                                                         >  1:33649/1‑213 (MQ=255)
                                                                                             ccAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTtctac                                                                                                                                                                                  >  1:477599/1‑219 (MQ=255)
                                                                                                aaCGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATg                                                                                                                                               <  1:261739/251‑1 (MQ=255)
                                                                                                     cTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTAc                                                                                                                                          <  1:663205/251‑1 (MQ=255)
                                                                                                        atatTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTAc                                                                                                                                                                <  1:793242/226‑1 (MQ=255)
                                                                                                        atatTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCg                                                                                                                                        <  1:176437/250‑1 (MQ=255)
                                                                                                          atTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGtt                                                                                                                                      >  1:691134/1‑250 (MQ=255)
                                                                                                                    tGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           <  1:203761/219‑1 (MQ=255)
                                                                                                                     gATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTg                                                                                                                           >  1:849423/1‑250 (MQ=255)
                                                                                                                          aagaCGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATAc                                                                                                                     <  1:858974/251‑1 (MQ=255)
                                                                                                                             aCGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTa                                                                                                                  <  1:811512/251‑1 (MQ=255)
                                                                                                                              cGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTc                                                                                                                                                                                                                  <  1:653413/154‑1 (MQ=255)
                                                                                                                                gTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAc                                                                                                                                                                                                                                                  >  1:822862/1‑121 (MQ=255)
                                                                                                                                gTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATg                                                                                                                                               >  1:84017/1‑219 (MQ=255)
                                                                                                                                gTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAAc                                                                                                                <  1:610110/250‑1 (MQ=255)
                                                                                                                                gTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACa                                                                                                               >  1:2063/1‑251 (MQ=255)
                                                                                                                                 tGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAg                                                                                                              <  1:819691/251‑1 (MQ=255)
                                                                                                                                   aTTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTCTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGAt                                                                                                            <  1:740434/251‑1 (MQ=255)
                                                                                                                                    tttCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           >  1:892349/1‑203 (MQ=255)
                                                                                                                                    tttCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGAtg                                                                                                           >  1:298440/1‑251 (MQ=255)
                                                                                                                                    tttCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGAtg                                                                                                           <  1:601043/251‑1 (MQ=255)
                                                                                                                                      tCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCCACGTGGTGTTTTTTCATTTAAGGTTACCTTTTGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGAtgtg                                                                                                         >  1:398701/1‑251 (MQ=255)
                                                                                                                                             cGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATg                                                                                                  <  1:579130/251‑1 (MQ=255)
                                                                                                                                              gAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAg                                                                                                              >  1:79973/1‑238 (MQ=255)
                                                                                                                                                   cccGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAg                                                                                                                                                         <  1:501336/190‑1 (MQ=255)
                                                                                                                                                    ccGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTTGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCag                                                                                           <  1:649699/251‑1 (MQ=255)
                                                                                                                                                    ccGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCag                                                                                           <  1:144804/251‑1 (MQ=255)
                                                                                                                                                    ccGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCag                                                                                           <  1:551068/251‑1 (MQ=255)
                                                                                                                                                      ggATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACt                                                                                                                                            >  1:700820/1‑200 (MQ=255)
                                                                                                                                                       gATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAAttt                                                                                                                                                                                           >  1:535316/1‑152 (MQ=255)
                                                                                                                                                        aTACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCaaa                                                                                                                                                                                                          <  1:750728/136‑1 (MQ=255)
                                                                                                                                                               aGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAtt                                                                                 <  1:385971/250‑1 (MQ=255)
                                                                                                                                                               aGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAtt                                                                                 <  1:844789/250‑1 (MQ=255)
                                                                                                                                                                 cGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAttg                                                                                <  1:512696/249‑1 (MQ=255)
                                                                                                                                                                 cGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAttg                                                                                <  1:328336/249‑1 (MQ=255)
                                                                                                                                                                   ggCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAg                                                                                                              >  1:112743/1‑217 (MQ=255)
                                                                                                                                                                     cttcttGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCa                                                                          >  1:690324/1‑251 (MQ=255)
                                                                                                                                                                                   aTCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATtattat                                                            <  1:417280/251‑1 (MQ=255)
                                                                                                                                                                                    tCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATg                                                                                                                                               <  1:32608/167‑1 (MQ=255)
                                                                                                                                                                                    tCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATtattat                                                            >  1:440966/1‑250 (MQ=255)
                                                                                                                                                                                       gAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCCATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAttg                                                                                <  1:449042/227‑1 (MQ=255)
                                                                                                                                                                                          tatCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTGCTCTACCTGTTTGGAGTACTATGCAGACTTCCAATg                                                                                                                                               <  1:840407/161‑1 (MQ=255)
                                                                                                                                                                                               aCGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATTATTATCATTTACaaaaa                                                >  1:48838/1‑251 (MQ=255)
                                                                                                                                                                                                    gtgtTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATAc                                                                                                                     >  1:267778/1‑177 (MQ=255)
                                                                                                                                                                                                    gtgtTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAg                                                                     <  1:583360/225‑1 (MQ=255)
                                                                                                                                                                                                      gtTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTAc                                                                                                                                          >  1:749579/1‑154 (MQ=255)
                                                                                                                                                                                                             cATTTAAGGTTTCCTTTGGCAGATTAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATTCCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAAttg                                                                                <  1:94872/205‑1 (MQ=255)
                                                                                                                                                                                                               tttAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAg                                                                                                              <  1:347566/173‑1 (MQ=255)
                                                                                                                                                                                                                   aGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAACAAAAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTc                                                                                                                               >  1:519093/1‑153 (MQ=255)
                                                                                                                                                                                                                         ccTTTGGCAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           <  1:273342/118‑1 (MQ=255)
                                                                                                                                                                                                                                cAGATGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAAttatta                                                             >  1:877036/1‑205 (MQ=255)
                                                                                                                                                                                                                                   aTGAGGATGTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGt                                                                                                 <  1:683962/166‑1 (MQ=255)
                                                                                                                                                                                                                                           gTCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATg                                                                                                  <  1:567208/157‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGAtg                                                                                                           <  1:365159/147‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCag                                                                                           <  1:247634/163‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATTATTATCa                                                          <  1:713550/196‑1 (MQ=255)
                                                                                                                                                                                                                                             cGCTATTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATTATTATCATTTACAAAAAATATTTTAATTACCGGAAATTTTCATATTTTCCAAGAGAATAGAAc  <  1:77750/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 aTTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGc                                                                                                                                                           <  1:299666/94‑1 (MQ=255)
                                                                                                                                                                                                                                                 aTTCTAACA‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATAc                                                                                                                     >  1:131504/1‑132 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               
AGGTCGTCATAAATTCAATTTCTACCGGCAAATGCAGTTCGCGCACCAAATCCGCCATGAACATAAAGGAACCGCGCAATAAGCCTACCACAACCAAACGGCTGATATTTTTGTGTTGATAGAAGACGGTGATTTCTTTACCGAGTTCCCGGATACGGGAGCGGGCTTCTTGTTCGGAAATCAGAATATCAACGTGGTGTTTTTTCATTTAAGGTTACCTTTGGCAGATGAGGATGTCGCTATTCTAAC‑‑AAAAAAAAGACTGAAGCTTAAACTTCAGTCTTTTCAAATACCCTAACGAATTTACTTTCTACTCTACCTGTTTGGAGTACTATGCAGACTTCCAATGACTACCGTTCATCTTCATTGCAATACTTAACAGATGTGGCTTATGTTAGCAGAGTTTTAATTGTTGTCAATAAGAATTATTATCATTTACAAAAAATATTTTAATTACCGGAAATTTTCATATTTTCCAAGAGAATAGAAC  >  1/146046‑146532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑