| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 133,102 | +CC | coding (475/561 nt) | unknown ← | Type IV fimbrial assembly, ATPase PilB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 133,102 | 1 | . | C | 100.0% | 197.2 / NA | 72 | R159G (CGT→GGT) | unknown | Type IV fimbrial assembly, ATPase PilB |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (45/27); total (45/27) | |||||||||||
| * | 1 | 133,102 | 2 | . | C | 98.6% | 189.9 / NA | 72 | R159G (CGT→GGT) | unknown | Type IV fimbrial assembly, ATPase PilB |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (45/26); minor base T (0/1); total (45/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATGCCCAGTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAATGGTGTTTTGCACCTGCAGCACG‑‑GTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTGGCTTTCAATTA > 1/132854‑133341 || aTGCCCAGTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTTGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGGTAAGGGAACGGTGTTTTTCACCTGCAGCACgc > 1:538106/1‑249 (MQ=255) cAGTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTTGCCAATATTCAGGTTTGCCAATAATTTTAAACGAGAAATTAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACgccgtcaa > 1:127631/1‑244 (MQ=255) gTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACgccgtcaatg > 1:673131/1‑242 (MQ=255) gTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACgccgtcaatg > 1:303087/1‑242 (MQ=255) gTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGGGTTTTGCACCTGCAGCACgccgtcaatg > 1:838644/1‑242 (MQ=255) ttCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACgccgtcaatg < 1:710375/250‑10 (MQ=255) ccGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGaaa > 1:238877/1‑251 (MQ=255) gTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTg > 1:844724/1‑250 (MQ=255) aTTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTgcag < 1:749395/240‑1 (MQ=255) aTTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCtt > 1:285935/1‑251 (MQ=255) aTTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCt < 1:874976/250‑1 (MQ=255) aaCGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGa < 1:801912/251‑1 (MQ=255) aCGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGGTCCAGAt > 1:418473/1‑251 (MQ=255) aCGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGGGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGATTGCAGGCAGTCTGCTTGCGGTTCCAGAt > 1:573150/1‑251 (MQ=255) cGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATg > 1:678000/1‑251 (MQ=255) aCCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAatat < 1:852864/250‑1 (MQ=255) cTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTg < 1:177051/225‑1 (MQ=255) tttttCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAgg < 1:544826/251‑1 (MQ=255) gAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGAt > 1:485121/1‑230 (MQ=255) gAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTtgtg > 1:792854/1‑251 (MQ=255) gggTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCa > 1:894300/1‑251 (MQ=255) gggTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGGTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATAACGGAGGCTTTTTGTGCCGTCGCGCTTTCa > 1:507974/1‑251 (MQ=255) ggAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGTGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCaaaaa > 1:384147/1‑251 (MQ=255) aaTCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAAc < 1:692000/251‑1 (MQ=255) aaTCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAAc < 1:250702/251‑1 (MQ=255) gAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCgg > 1:19666/1‑251 (MQ=255) cgtTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCAt < 1:294002/251‑1 (MQ=255) gtTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGGCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATa > 1:78878/1‑251 (MQ=255) tGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTgtgt < 1:452116/251‑1 (MQ=255) gtcTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACgg < 1:322521/234‑1 (MQ=255) tcTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAata < 1:879791/162‑1 (MQ=255) tcTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTc > 1:693536/1‑248 (MQ=255) ttGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCtgtg < 1:140340/251‑1 (MQ=255) cGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGTTAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc < 1:523999/250‑1 (MQ=255) cGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc < 1:652414/250‑1 (MQ=255) cGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc < 1:421215/250‑1 (MQ=255) cGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc < 1:189630/250‑1 (MQ=255) cGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACa > 1:296764/1‑251 (MQ=255) ggCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCg > 1:656031/1‑251 (MQ=255) ggTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAAt > 1:207211/1‑251 (MQ=255) cGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAag > 1:533896/1‑251 (MQ=255) cGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAag < 1:108278/251‑1 (MQ=255) cTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCTGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAag < 1:30788/249‑1 (MQ=255) cTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTc > 1:723226/1‑203 (MQ=255) cTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACgg < 1:289217/212‑1 (MQ=255) cTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAagag < 1:42089/251‑1 (MQ=255) atatCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCgg > 1:14536/1‑251 (MQ=255) atatCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCgg > 1:872799/1‑251 (MQ=255) atCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc > 1:820412/1‑229 (MQ=255) atCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc > 1:861044/1‑229 (MQ=255) cGAGAAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTcc > 1:190687/1‑198 (MQ=255) agaAATCAGTCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAg > 1:7005/1‑115 (MQ=255) tCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAAt < 1:524904/251‑1 (MQ=255) tCGGCTCGCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAAt < 1:205118/251‑1 (MQ=255) gCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCaa > 1:361302/1‑251 (MQ=255) gCTATGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCaa > 1:720149/1‑251 (MQ=255) aTGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAagag < 1:856152/200‑1 (MQ=255) aTGGATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAag > 1:269626/1‑198 (MQ=255) ggATAAGGGAGCGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCg < 1:798327/146‑1 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAat > 1:554505/1‑87 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCt > 1:837859/1‑170 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCtgtg > 1:165094/1‑179 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAAt < 1:752624/239‑1 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAAt > 1:223848/1‑239 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCgg > 1:828923/1‑251 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCgg > 1:691023/1‑251 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCgg > 1:398741/1‑251 (MQ=255) ggATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCgg > 1:867979/1‑251 (MQ=255) gATAAGGGAACGGTGTTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTcc > 1:154367/1‑174 (MQ=255) gtgtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTg > 1:610792/1‑251 (MQ=255) gtgtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTg > 1:131191/1‑251 (MQ=255) gtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGAc < 1:95095/166‑1 (MQ=255) gtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTgg > 1:232405/1‑250 (MQ=255) gtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTgg > 1:534341/1‑250 (MQ=255) gtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTgg > 1:590012/1‑250 (MQ=255) gtTTTGCACCTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTACGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTgg > 1:430899/1‑250 (MQ=255) cTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTGGCTTTCAATTa > 1:297052/1‑251 (MQ=255) cTGCAGCACGCCGTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGATCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTGGCTTTCAATTa > 1:43306/1‑251 (MQ=255) || ATGCCCAGTTCACCGAATGCCAGTTGCACGGGACGATTTTGTTGCAAACGCAGGACCGCTTTTTCACCGAATTGGGTGGCAAGGGTGGAAAGGCGAAAATCCAGCGTGTCGGAAAACGTCGTTTTAAACTGAAAGCGTCCGTCTTGCGGCAGGCGGGTTTCGCTAATATCCAGTTTTGCCAATAATTTTAAACGAGAAATCAGTCGGCTCGCTATGGATAAGGGAATGGTGTTTTGCACCTGCAGCACG‑‑GTCAATGCGAAAACGGGTTTGCAGGCAGTCTGCTTGCGGTTCCAGATGAATATCGGAGGCTTTTTGTGCCGTCGCGCTTTCAAAAATATCATTTAACAGCCGAATAATCGGTTCGTCATACGGTTGTGTCGGCTGTTCCTGTGACAGTGCGGAATAAAAGAGCGGTTGATTTTCTTCGACTTTTTCGACGCGCGGGGATAAATCCTGCAATGCGGTTTTTAGCGTTTGGCTTTCAATTA > 1/132854‑133341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |