Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	804,288	(T)5→4	intergenic (+88/‑91)	unknown → / → unknown	5'‑methylthioadenosine nucleosidase (EC 3.2.2.16) @ S‑adenosylhomocysteine nucleosidase (EC 3.2.2.9)/Additional periplasmic component NikK of nickel ECF transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	804,284	0	T	.	100.0%	167.4 / NA	40	intergenic (+84/‑95)	unknown/unknown	5'‑methylthioadenosine nucleosidase (EC 3.2.2.16) @ S‑adenosylhomocysteine nucleosidase (EC 3.2.2.9)/Additional periplasmic component NikK of nickel ECF transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/21);  total (19/21)

CTATTGCGCAAGTTTGCCATGCGTTTAACGTGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCATTTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCGGGCATGAACAAACGG  >  1/804037‑804520                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             cTATTGCGCAAGTTTGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAttt                                                                                                                                                                                                                                            <  1:632758/250‑1 (MQ=255) cTATTGCGCAAGTTTGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAttt                                                                                                                                                                                                                                            <  1:751400/250‑1 (MQ=255) cTATTGCGCAAGTTTGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAt                                                                                                                                                                                                                                              <  1:38841/248‑1 (MQ=255)         cAAGTTTGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTAt                                                                                                                                                                                                                                   <  1:469596/250‑1 (MQ=255)           aGTTTGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGTGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTAtttt                                                                                                                                                                                                                                >  1:286361/1‑251 (MQ=255)               tGCCATGCGTTTAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTtctc                                                                                                                                                                                                                            <  1:564412/251‑1 (MQ=255)                        tttAACGCGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAaca                                                                                                                                                                                                                   >  1:806926/1‑251 (MQ=255)                                     tCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTGATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:372556/251‑1 (MQ=255)                                     tCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:895956/251‑1 (MQ=255)                                     tCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:263803/251‑1 (MQ=255)                                     tCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:642534/251‑1 (MQ=255)                                     tCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:750700/251‑1 (MQ=255)                                             gtgCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAtt                                                                                                                                                                                                                                             <  1:211843/205‑1 (MQ=255)                                                  cgcCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:852568/238‑1 (MQ=255)                                                     cATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTttat                                                                                                                                                                                      <  1:169152/251‑1 (MQ=255)                                                      aTTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTttat                                                                                                                                                                                      <  1:437329/250‑1 (MQ=255)                                                       tttCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCat                                                                                                                                                                                             <  1:400788/242‑1 (MQ=255)                                                          ccGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGCCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATgg                                                                                                                                                                                 <  1:594628/251‑1 (MQ=255)                                                                  ggCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTGCCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      <  1:361468/222‑1 (MQ=255)                                                                  ggCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATAtt                                                                                                                                                                                          >  1:521132/1‑234 (MQ=255)                                                                    cGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAAttt                                                                                                                                                                       <  1:77219/251‑1 (MQ=255)                                                                                 aaTATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAg                                                                                                                                                          >  1:898497/1‑251 (MQ=255)                                                                                  atatGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAgg                                                                                                                                                         <  1:631227/251‑1 (MQ=255)                                                                                   tatGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAg                                                                                                                                                                                                      >  1:71232/1‑205 (MQ=255)                                                                                             gaagaaTTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAttt                                                                                                                                                                                                                                            <  1:660790/158‑1 (MQ=255)                                                                                                                  gCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCAt                                                                                                                                                                                                                                              <  1:21373/135‑1 (MQ=255)                                                                                                                  gCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCg                                                                                                                          >  1:409595/1‑250 (MQ=255)                                                                                                                                  aCATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACtt                                                                                                         >  1:354812/1‑251 (MQ=255)                                                                                                                                          gTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCgg                                                                                                  >  1:719627/1‑250 (MQ=255)                                                                                                                                                 tGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGtt                                                                                          >  1:123613/1‑251 (MQ=255)                                                                                                                                                  gTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTg                                                                                         >  1:188965/1‑251 (MQ=255)                                                                                                                                                              gAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTcaca                                                                             >  1:890978/1‑251 (MQ=255)                                                                                                                                                                           aTTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGa                                                                >  1:419222/1‑251 (MQ=255)                                                                                                                                                                             tttCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAAc                                                              <  1:803460/251‑1 (MQ=255)                                                                                                                                                                                cccTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGaa                                                               <  1:119592/247‑1 (MQ=255)                                                                                                                                                                                    ctGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCaa                                                       >  1:42589/1‑251 (MQ=255)                                                                                                                                                                                      gCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCTTATTTTTCTCAAAAAAACACTTGACTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGAcc                                                                                                               <  1:8933/193‑1 (MQ=255)                                                                                                                                                                                       cATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAagga                                                    >  1:899805/1‑251 (MQ=255)                                                                                                                                                                                                                     cgGAAACCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGa                       <  1:353841/250‑1 (MQ=255)                                                                                                                                                                                                                        aaaCCTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTg                                                                  <  1:483511/204‑1 (MQ=255)                                                                                                                                                                                                                           ccTAATTATCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCgg                >  1:558605/1‑251 (MQ=255)                                                                                                                                                                                                                                   tCCACACACTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTGCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGa                       <  1:449215/236‑1 (MQ=255)                                                                                                                                                                                                                                      acacacTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTg                        >  1:399936/1‑232 (MQ=255)                                                                                                                                                                                                                                      acacacTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCGGGCATGAACaaa     >  1:323036/1‑251 (MQ=255)                                                                                                                                                                                                                                         cacTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCGGGCATGAACAAACgg  >  1:556593/1‑251 (MQ=255)                                                                                                                                                                                                                                         cacTTATCTCTCTCA‑TTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCGGGCATGAACAAACgg  >  1:688245/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             CTATTGCGCAAGTTTGCCATGCGTTTAACGTGCCTTTCGTGGTGGTGCGCGCCATTTCCGATGCGGGCGATGGCAAAGCTAATATGTCTTTTGAAGAATTTCTACCGCTCGCTGCCAAACAATCGTCCGACATGGTTGTCGCCATGTTGGCGGAGTTGAATTAACGGTTTATTTTCCCTCTGCATTCCCGCCGCGTACGCGTTTGCCGTACGCGGAAACCTAATTATCCACACACTTATCTCTCTCATTTTTCCCGTATTTTTCTCAAAAAAACACTTGATTCTAAAGTTACTTCATATTTTATTATGGTATCAAATTTTAATTGATAAAAGGAGAGATCACATGAAATTCGAAAAAACAACCGCTATTTTGACCGCACTTTTCGCGGCATCCGTTGCTAACGCTCACAACGTATGGCTGGAACCCGTCAAGGAGGCGAATTCTACCGGGCAATATGTAGTGAAATTCGGGCATGAACAAACGG  >  1/804037‑804520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑