Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,647,504	Δ1 bp	coding (274/339 nt)	unknown ←	Transposase, IS200 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,647,504	0	G	.	100.0%	142.7 / NA	35	coding (274/339 nt)	unknown	Transposase, IS200 family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/15);  total (20/15)

AATACAAACAGTTAAATCTCATAAACAAACTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTGAAAGGGGTCCACATATCCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTCCACTGTCTTTGTATTTAGCCCTACCG  >  1/1647272‑1647608                                                                                                                                                                                                                                         |                                                                                                         aaTACAAACAGTTAAATCTCATAAACAAACTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACatatt                                                                                         >  1:465872/1‑248 (MQ=255)                        aacaaacTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAAt                                                                <  1:631322/251‑1 (MQ=255)                            aacTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACatattc                                                                                        >  1:716840/1‑221 (MQ=255)                              cTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAgg                                                                                                     >  1:13379/1‑208 (MQ=255)                                ataataATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACCAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCtt                                                        >  1:519128/1‑251 (MQ=255)                                ataataATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGGTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAgg                                                                                                     <  1:462278/206‑1 (MQ=255)                                    taatCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAAt                                                                          >  1:108958/1‑229 (MQ=255)                                       tCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCtttt                                                  >  1:5896/1‑250 (MQ=255)                                        cTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAgg                                                                                                     >  1:130920/1‑198 (MQ=255)                                                acttactGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTc                                          >  1:127330/1‑249 (MQ=255)                                                     ctGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTtgtat                                   <  1:800039/251‑1 (MQ=255)                                                     ctGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTtatat                                   >  1:299360/1‑251 (MQ=255)                                                       gCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTtatata                                  >  1:315518/1‑250 (MQ=255)                                                        ccGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATAc                                 <  1:444977/250‑1 (MQ=255)                                                         cGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATcc                                                                     >  1:142111/1‑213 (MQ=255)                                                           aaCTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACat                                                                                            <  1:625783/188‑1 (MQ=255)                                                           aaCTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATActcc                              <  1:308845/250‑1 (MQ=255)                                                           aaCTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATActc                               >  1:830410/1‑249 (MQ=255)                                                               aaCAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTacac                         <  1:74541/251‑1 (MQ=255)                                                               aaCAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTacac                         <  1:178769/251‑1 (MQ=255)                                                                 cAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAgg                                                                                                     >  1:849816/1‑173 (MQ=255)                                                                 cAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAAt                                                                <  1:171817/210‑1 (MQ=255)                                                                 cAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTacac                         <  1:654396/249‑1 (MQ=255)                                                                  agttagttCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTc                                          >  1:359192/1‑231 (MQ=255)                                                                        ttCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACtacctttg                 <  1:555476/250‑8 (MQ=255)                                                                            ggTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTAt              >  1:91189/1‑249 (MQ=255)                                                                                aaaTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACatat                                                                                          >  1:421892/1‑169 (MQ=255)                                                                                aaaTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTcc                                                      >  1:525547/1‑205 (MQ=255)                                                                                aaaTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATAct                                >  1:224001/1‑227 (MQ=255)                                                                                aaaTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGcc        <  1:88441/251‑1 (MQ=255)                                                                                  aTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATAc                                 >  1:527362/1‑224 (MQ=255)                                                                                  aTTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGcc        >  1:173696/1‑249 (MQ=255)                                                                                    tACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTAc    <  1:239399/251‑1 (MQ=255)                                                                                      cacCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTACCg  <  1:901509/251‑1 (MQ=255)                                                                                                                  cAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTT‑AAAgg                                                                                                     <  1:459200/124‑1 (MQ=17)                                                                                                                                                                                                                                         |                                                                                                         AATACAAACAGTTAAATCTCATAAACAAACTATAATAATCTTTGTTTACTTACTGCCGAACTAACAGTTAGTTCAGGTAAAATTACACCCTGTACAAAAAAATCATAAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTGAAAGGGGTCCACATATCCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTCCACTGTCTTTGTATTTAGCCCTACCG  >  1/1647272‑1647608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑