Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,477,748	Δ1 bp	intergenic (‑332/‑417)	unknown ← / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,477,745	0	A	.	100.0%	63.1 / NA	16	intergenic (‑329/‑420)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/Flp pilus assembly protein, pilin Flp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (8/8);  total (8/8)

TGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCA  >  1/1477501‑1477939                                                                                                                                                                                                                                                     |                                                                                                                                                                                                     tGCACGGTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaatata                                                                                                                                                                                                >  1:407675/1‑246 (MQ=255)     cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                                                                                                                                                                   <  1:194500/241‑2 (MQ=255)     cggTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                                                                                                                                                                   <  1:787465/241‑2 (MQ=255)       gTAAAGGTAAACCCACAGCGGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATAc                                                                                                                                                                                              <  1:488328/245‑1 (MQ=255)                 acccacagcgGGCAACAGAAAGATCAAAATGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCATaaat                                                                                                                                                                                                   <  1:408661/223‑2 (MQ=255)                                             aTGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTaaaaaa                                                                                                                                                  >  1:552195/1‑251 (MQ=255)                                              tGTTTTTTAATTAAAG‑AAAGGTGTTTATCCCCCGCCCCGCATAAAGAACGTAAACGCTTGTTATCTAGCGGTTCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAg                                                                                                                                                 <  1:48135/251‑1 (MQ=255)                                                                                                                    ggttCAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTaaaaa                                                                                                                                                   >  1:207156/3‑180 (MQ=255)                                                                                                                        cAAGAGTGAAAGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAg                                                                        <  1:563103/251‑1 (MQ=255)                                                                                                                                 aaGTTTGGCTAAATTGCTTGTTCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGtg                                                                      >  1:578954/1‑243 (MQ=255)                                                                                                                                               tgcttgttCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCcacaca                                                                                >  1:813579/4‑220 (MQ=255)                                                                                                                                                    gttCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATAC‑ttttt                                                                                                                                                                                        >  1:725840/1‑110 (MQ=255)                                                                                                                                                     ttCAAAACCTTGCCTCAAAAGTGAGCAAAAAAGTGGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTaaa                                                                                        >  1:702739/1‑206 (MQ=255)                                                                                                                                                                                        gCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAAt        <  1:276276/250‑1 (MQ=255)                                                                                                                                                                                             cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGAttt                                                                                                                                                                 <  1:155335/93‑1 (MQ=255)                                                                                                                                                                                             cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGtg                                                                      <  1:66297/184‑2 (MQ=255)                                                                                                                                                                                             cAATATAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGT‑GTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGAGAAAGTAGAAATAGCTCa  >  1:654655/1‑251 (MQ=255)                                                                                                                                                                                               atatAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATAc                                                                                                                                                                                              <  1:688566/62‑1 (MQ=255)                                                                                                                                                                                               atatAGCACAATTTGCATCGAAAGAGACTAGATGCAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAg                                                                                                                                                 <  1:761698/107‑1 (MQ=255)                                                                                                                                                                                                                                 cAATAAAATTGCTCTTCCAT‑AAATATACTTTTTTAGAAAAAAAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACGACTTTCCTACAAAGAAAAAAGAAATAAATCGCGATATCTCTAAATCCACACATATTTAAGTGtttttg                                                                >  1:438452/1‑153 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                     TGCACAGTAAAGATAAAACCACGACGGGTAACAGAAAAACTAAAATGTTTTTTAATTAAAGAAAAGGTGTTTATCCCCCGCTCCGCATAAAAAACGTAAACGCTTGCTATCTTGCGATTCAAGAGTGAAAGTTCGGTTAAATTGTTTGTTCAAAACCCTGCCTCAAAAGTGAACAAAAAAGTAGCAGACAATATAGCACAATTTGCATCGAAAGAGACTAGACGCAATAAAATTGCTCTTCCATAAAATATAC‑TTTTTAGAAAAACAACAAAATTTGATTTAGTTAAGTTAAAAAAGCGTAAAAAATACAACTTTCCTAC‑AAGGAAAAAGAAATAAATCGCGACATCTCTAAATCCACACATATTTAAGTATTTTTTGTAATTAAAGTTAAAAGTTGTAAATTTACTGTAAAATCAATAGGGAAAGTAGAAATAGCTCA  >  1/1477501‑1477939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑