Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,658	Δ1 bp	coding (1420/1923 nt)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,658	0	C	.	100.0%	263.5 / NA	63	coding (1420/1923 nt)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/36);  total (27/36)

CGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTC  >  1/1474425‑1474899                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   cGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                                                                                                                                                                                                   <  1:810302/250‑1 (MQ=255)   ggtacaatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTg                                                                                                                                                                                                                                 >  1:709743/9‑250 (MQ=255)     tGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc                                                                                                                                                                                                                              <  1:476885/251‑1 (MQ=255)      gCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc                                                                                                                                                                                                                              >  1:704049/1‑250 (MQ=255)       caatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                                                                                                                                                                                                   >  1:881949/5‑244 (MQ=255)           gAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgcc                                                                                                                                                                                                                         >  1:447361/1‑248 (MQ=255)               ggggCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgccctcg                                                                                                                                                                                                                     >  1:465959/1‑244 (MQ=255)                  gcaaattgcGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCgctg                                                                                                                                                                                                                  <  1:819576/242‑1 (MQ=255)                      attgcGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGcc                                                                                                                                                                                                             <  1:168332/247‑1 (MQ=255)                              tAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTgggc                                                                                                                                                                                                     <  1:635730/251‑2 (MQ=255)                                      tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCTAGTTGGGCTGCACGGc                                                                                                                                                                                             <  1:562036/248‑1 (MQ=255)                                      tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGc                                                                                                                                                                                             <  1:602337/248‑1 (MQ=255)                                                          cgcccgccCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaag                                                                                                                                                                         <  1:289964/251‑4 (MQ=255)                                                            cccgcccgTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaaga                                                                                                                                                                        <  1:369461/250‑5 (MQ=255)                                                                cccgtgcTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                                                                                                                                                                                                   <  1:528975/182‑1 (MQ=255)                                                                          gttaGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgccc                                                                                                                                                                                                                        <  1:888813/185‑4 (MQ=255)                                                                           ttaGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGcc                                                                                                                                                        <  1:505795/249‑1 (MQ=255)                                                                             aGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaag                                                                                                                                                                         <  1:256412/232‑4 (MQ=255)                                                                                     caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAAt                                                                                                                                               >  1:687759/5‑250 (MQ=255)                                                                                     caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                              <  1:85014/247‑1 (MQ=255)                                                                                     caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                              <  1:827358/247‑1 (MQ=255)                                                                                                cGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATcc                                                                                                                                             <  1:808127/241‑1 (MQ=255)                                                                                                                     cATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCaaa                                                                                                              >  1:159577/1‑251 (MQ=255)                                                                                                                       tCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAg                                                                                                                                                                                                           <  1:504531/156‑1 (MQ=255)                                                                                                                                   cTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAAttgccgcgcaggcg                                                                                                <  1:27586/251‑13 (MQ=255)                                                                                                                                            cccAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                       <  1:101494/250‑1 (MQ=255)                                                                                                                                                aaaTAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAac                                                                                                                                  >  1:127976/1‑204 (MQ=255)                                                                                                                                                aaaTAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCcgcgt                                                                                   >  1:135587/1‑250 (MQ=255)                                                                                                                                                     aaaCCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATAc                                                                                                                                                                                        >  1:423762/1‑145 (MQ=255)                                                                                                                                                                               cagttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                              <  1:287907/157‑1 (MQ=255)                                                                                                                                                                               cagttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaa                                                    >  1:305880/5‑240 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTa                                                                                                                                                                                                                                        >  1:161642/3‑69 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCAc                                                                                                                                                                                                >  1:704660/3‑109 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAACCACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:446276/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACAt                                                                                                                           <  1:340791/176‑1 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAAtttgac                                                                         >  1:143746/3‑225 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:836462/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:543915/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:149617/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:578174/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                  >  1:217141/3‑238 (MQ=255)                                                                                                                                                                                 gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                             <  1:366912/238‑3 (MQ=255)                                                                                                                                                                                  ttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAACCGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatc                                                 <  1:770770/250‑15 (MQ=255)                                                                                                                                                                                      ggggTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                             >  1:848980/1‑233 (MQ=255)                                                                                                                                                                                          tgtAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatcctccttc                                          <  1:338311/250‑22 (MQ=255)                                                                                                                                                                                                        aCGGAAAGAAAGTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCGGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATGCGGGAAATGCGCAAAGATCGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcaca                            <  1:85052/250‑1 (MQ=255)                                                                                                                                                                                                        aCGGAAAGAAAATCCACCGCAGGTTGTTGTTTAA‑TCGGTTCGATTAAACGTGGCCCCAGCCCTCGCTGCGGCCAGTTGGGCTGCACGGCGATACGGAAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                           <  1:16077/251‑1 (MQ=255)                                                                                                                                                                                                        aCGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                           <  1:598662/251‑1 (MQ=255)                                                                                                                                                                                                         cGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatca                                                           >  1:390200/1‑214 (MQ=255)                                                                                                                                                                                                           gaaagaaaaTCCCCCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                         <  1:30271/250‑1 (MQ=255)                                                                                                                                                                                                           gaaagaaaaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                         <  1:892622/250‑1 (MQ=255)                                                                                                                                                                                                                  aaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaac                 <  1:386119/251‑1 (MQ=255)                                                                                                                                                                                                                    cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag               <  1:308059/250‑2 (MQ=255)                                                                                                                                                                                                                    cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag               <  1:596187/250‑2 (MQ=255)                                                                                                                                                                                                                          gCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAAttgc                                                                                                          <  1:569874/154‑3 (MQ=255)                                                                                                                                                                                                                            aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                         <  1:297852/233‑1 (MQ=255)                                                                                                                                                                                                                            aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcc        >  1:91252/1‑242 (MQ=255)                                                                                                                                                                                                                             cGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                           <  1:323252/230‑1 (MQ=255)                                                                                                                                                                                                                               ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                       <  1:489061/168‑1 (MQ=255)                                                                                                                                                                                                                               ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                         >  1:471552/1‑230 (MQ=255)                                                                                                                                                                                                                               ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                           >  1:437368/1‑228 (MQ=255)                                                                                                                                                                                                                               ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg    >  1:585054/1‑239 (MQ=255)                                                                                                                                                                                                                                 gttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtt  >  1:144890/1‑237 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTC  >  1/1474425‑1474899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑