Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,473,647	(T)7→6	intergenic (‑161/+294)	unknown ← / ← unknown	Transcription‑repair coupling factor/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,473,641	0	T	.	100.0%	133.9 / NA	43	intergenic (‑155/+300)	unknown/unknown	Transcription‑repair coupling factor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/22);  total (21/22)

GCTATCCGCACCGGGCAGGATATTGGCTAAAATTTTATGATCATTCGCTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTT  >  1/1473400‑1473882                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATAttttttaaat                                                                                                                                                                                                                                          >  1:361648/1‑247 (MQ=255)                  ggATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGt                                                                                                                                                                                                                        >  1:849332/1‑251 (MQ=255)                          gCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACggg                                                                                                                                                                                                                 <  1:790940/250‑1 (MQ=255)                               aaTTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACa                                                                                                                                                                                                           >  1:793962/1‑251 (MQ=39)                                        aTCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACggg                                                                                                                                                                                                                 <  1:616048/236‑1 (MQ=39)                                              cacTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGttt                                                                                                                                                                                            >  1:94864/3‑251 (MQ=38)                                                                             tGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                             <  1:598920/251‑1 (MQ=37)                                                                                                     agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGaa                                                                                                                                       <  1:123398/249‑1 (MQ=25)                                                                                                     agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAAc                                                                                                                                      <  1:454584/250‑1 (MQ=25)                                                                                                                     cagTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgggg                                                                                                                                                           <  1:440748/211‑1 (MQ=25)                                                                                                                           gTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATaa                                                                                                                            >  1:754674/1‑238 (MQ=21)                                                                                                                                               aTTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                                                            <  1:357275/250‑1 (MQ=255)                                                                                                                                                   ccAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                             <  1:285552/181‑1 (MQ=255)                                                                                                                                                    cAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGt                                                                                      >  1:807303/1‑251 (MQ=255)                                                                                                                                                      aTAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                                                                 >  1:607509/1‑158 (MQ=25)                                                                                                                                                             tCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCAATGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAg                                                                             <  1:686507/251‑1 (MQ=255)                                                                                                                                                                gTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGct                                                                                                                                                                                <  1:590411/149‑1 (MQ=21)                                                                                                                                                                 tataAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                                                            <  1:520546/232‑1 (MQ=255)                                                                                                                                                                              tAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAAttt                                                            >  1:621061/1‑250 (MQ=255)                                                                                                                                                                                aTTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATg                                                                                       <  1:551652/222‑1 (MQ=255)                                                                                                                                                                                     aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                             >  1:629812/1‑147 (MQ=18)                                                                                                                                                                                     aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAtttt                                                     >  1:679621/1‑250 (MQ=255)                                                                                                                                                                                          gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGc                                                                                                                                                                                                     <  1:616685/102‑1 (MQ=18)                                                                                                                                                                                          gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                                                                 <  1:16384/122‑1 (MQ=18)                                                                                                                                                                                          gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAAt                                                                                                                              <  1:540947/173‑1 (MQ=255)                                                                                                                                                                                          gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAttttgccca                                                >  1:824935/1‑245 (MQ=14)                                                                                                                                                                                                 gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                         >  1:845409/1‑251 (MQ=14)                                                                                                                                                                                                 gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                         >  1:50152/1‑251 (MQ=14)                                                                                                                                                                                                 gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                         >  1:705025/1‑251 (MQ=14)                                                                                                                                                                                                  cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                        <  1:368847/251‑1 (MQ=14)                                                                                                                                                                                                  cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGAGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                         <  1:126165/250‑1 (MQ=14)                                                                                                                                                                                                      gccccGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCg                                    >  1:493436/5‑251 (MQ=14)                                                                                                                                                                                                           gggTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                               >  1:392023/1‑251 (MQ=255)                                                                                                                                                                                                             gTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                               >  1:558716/1‑249 (MQ=14)                                                                                                                                                                                                                 tACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTcccc                         <  1:740927/251‑1 (MQ=255)                                                                                                                                                                                                                      ttgttCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGa                    <  1:668103/251‑1 (MQ=255)                                                                                                                                                                                                                        gttCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATg                                                                                       >  1:694365/1‑182 (MQ=255)                                                                                                                                                                                                                          tCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACgg                <  1:6479/251‑2 (MQ=14)                                                                                                                                                                                                                             aaaCATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCtt                                                                    <  1:87613/196‑1 (MQ=255)                                                                                                                                                                                                                                cATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGag          >  1:261645/1‑250 (MQ=14)                                                                                                                                                                                                                                  ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                        >  1:818221/1‑219 (MQ=11)                                                                                                                                                                                                                                  ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                  >  1:389946/1‑241 (MQ=14)                                                                                                                                                                                                                                      aaTTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGt    <  1:879846/251‑1 (MQ=14)                                                                                                                                                                                                                                        ttCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGttt  >  1:62052/1‑251 (MQ=14)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GCTATCCGCACCGGGCAGGATATTGGCTAAAATTTTATGATCATTCGCTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTT  >  1/1473400‑1473882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑