Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	729,216	2 bp→CG	intergenic (‑6502/‑57974)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	729,216	0	T	C	100.0%	86.7 / NA	31	intergenic (‑6502/‑57975)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/12);  total (19/12)
*	1	729,217	0	C	G	100.0%	87.6 / NA	31	intergenic (‑6503/‑57974)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/12);  total (19/12)

GTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCG  >  1/728975‑729441                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                 gtcttCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                                                                                                                                                                           <  1:641658/247‑1 (MQ=255)     tCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGttt                                                                                                                                                                                                                      <  1:891812/251‑1 (MQ=255)           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                                                                                                                                                                           <  1:837591/239‑1 (MQ=255)                     gTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGaa                                                                                                                                                                                                       >  1:77978/1‑250 (MQ=255)                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                                                                                                                                                      <  1:177452/250‑1 (MQ=255)                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                                                                                                                                                      <  1:860768/250‑1 (MQ=255)                         cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcg                                                                                                                                                                                                  >  1:579153/1‑251 (MQ=255)                           ttCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgt                                                                                                                                                                                                 >  1:832531/1‑249 (MQ=255)                                aactacatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaa                                                                                                                                                                                            <  1:536029/242‑7 (MQ=255)                                    acatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaagattg                                                                                                                                                                                       >  1:529655/5‑240 (MQ=255)                                     catcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTgg                                                                                                                                                                                      >  1:536541/4‑251 (MQ=255)                                               ccTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATccg                                                                                                                                                                            >  1:450363/1‑250 (MQ=255)                                                    gAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTAtat                                                                                                                                                                                                                  >  1:418508/1‑206 (MQ=255)                                                     aaCAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCaa                                                                                                                                                                      <  1:14588/251‑1 (MQ=255)                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                >  1:897422/1‑251 (MQ=255)                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                >  1:272634/1‑251 (MQ=255)                                                                         cAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTAtt                                                                                                                                                  <  1:834540/251‑1 (MQ=255)                                                                              ggcgacatccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTgg                                                                                                                                             >  1:68449/17‑251 (MQ=255)                                                                                     tccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgc                                                                                                                                      <  1:278547/242‑1 (MQ=255)                                                                                      ccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgcg                                                                                                                                     >  1:443875/9‑251 (MQ=255)                                                                                              gagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCgatgat                                                                                                                             >  1:694667/1‑251 (MQ=255)                                                                                                                                                                                                        tgAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTc                   <  1:234747/250‑1 (MQ=255)                                                                                                                                                                                                         gAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCa                  >  1:856567/1‑251 (MQ=255)                                                                                                                                                                                                                    cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac        <  1:803534/250‑10 (MQ=255)                                                                                                                                                                                                                    cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac        <  1:348820/250‑10 (MQ=255)                                                                                                                                                                                                                      gTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccga     >  1:265432/1‑251 (MQ=255)                                                                                                                                                                                                                        caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGTTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac    >  1:872472/5‑250 (MQ=255)                                                                                                                                                                                                                        caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac    >  1:777083/5‑250 (MQ=255)                                                                                                                                                                                                                        caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac    >  1:196735/5‑250 (MQ=255)                                                                                                                                                                                                                        caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac    >  1:408353/5‑250 (MQ=255)                                                                                                                                                                                                                          ataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGGTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGCTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgaccg  >  1:879924/3‑250 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                 GTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCG  >  1/728975‑729441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑