Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	728,547	2 bp→CT	intergenic (‑5833/‑58643)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	728,547	0	G	C	100.0%	104.7 / NA	35	intergenic (‑5833/‑58644)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/12);  total (23/12)
*	1	728,548	0	C	T	100.0%	96.3 / NA	35	intergenic (‑5834/‑58643)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/12);  total (23/12)

AACGAACGCAAACGCCGGCAAGGCTTGGCGGGCGACAGTCGTTCTGTTGAAAACCAAGTGCAAAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTG  >  1/728330‑728774                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                   aacgaacgTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttctcattggtga                                                                                                                                                                                                    <  1:585950/251‑13 (MQ=255)     aacgTAAACGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGAt                                                                                                                                                                                                >  1:259635/1‑251 (MQ=255)             cGCCGTCAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGtt                                                                                                                                                                                        <  1:42654/251‑1 (MQ=255)                 gtcAAGGCTTGGCGGGTGACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAgcg                                                                                                                                                                                    >  1:709852/3‑251 (MQ=255)                               ggtgACAGTCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttc                                                                                                                                                                                                              <  1:88790/208‑3 (MQ=255)                                       tCGTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGaacatttt                                                                                                                                                                  <  1:444568/247‑7 (MQ=255)                                         gTTCCGTTGAAAACCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGc                                                                                                                                                             >  1:486308/1‑250 (MQ=255)                                                    aaCCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTg                                                                                                                                                  >  1:246518/1‑250 (MQ=255)                                                     aCCAAATGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCg                                                                                                                                                >  1:573032/1‑251 (MQ=255)                                                         aatGCAGAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGgtat                                                                                                                                            <  1:53669/249‑3 (MQ=255)                                                                aaGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGAt                                                                                                                                        <  1:210647/248‑1 (MQ=255)                                                                   cAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATTCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCgg                                                                                                                                  <  1:214952/251‑1 (MQ=255)                                                                            aCGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                                                          >  1:889965/1‑247 (MQ=255)                                                                                           gcatttggTTTTCA‑ACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCAc                                                                                                                     <  1:123204/232‑1 (MQ=255)                                                                                              cGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCagcc                                                                                                       <  1:367699/251‑4 (MQ=255)                                                                                                             cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGtttcac                                                                                                                          <  1:5892/217‑4 (MQ=255)                                                                                                             cAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAg                                                                                        >  1:572754/1‑251 (MQ=255)                                                                                                               aCGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCa                                                                                      >  1:198051/1‑251 (MQ=255)                                                                                                                    aaTCTATTGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATtcttctcattggtga                                                                                                                                                                                                    >  1:164543/1‑124 (MQ=255)                                                                                                                          ttGAACGCCAAATTCGCGGCGAAAGTGAACCGCTCTATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAAt                                                                           <  1:108532/251‑1 (MQ=255)                                                                                                                                                          ctctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCTCGCGCTTATTTa                                           >  1:467338/1‑251 (MQ=255)                                                                                                                                                            ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCttatttat                                          >  1:154465/1‑250 (MQ=255)                                                                                                                                                            ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                         >  1:590757/1‑251 (MQ=255)                                                                                                                                                            ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                         >  1:823761/1‑251 (MQ=255)                                                                                                                                                            ctATAAAAGCAATAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatg                                         >  1:11930/1‑251 (MQ=255)                                                                                                                                                                       aTAAAAATATTAATGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGgcgtcag                              >  1:160657/1‑247 (MQ=255)                                                                                                                                                                              tattaaTGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTccc                                                       >  1:577465/5‑219 (MQ=255)                                                                                                                                                                                  aaTGTAGATTGGACGGATCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGgcgtcag                              >  1:558484/1‑236 (MQ=255)                                                                                                                                                                                            ggacggatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGctc           >  1:28446/5‑247 (MQ=255)                                                                                                                                                                                                 gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatga                                        <  1:373470/215‑1 (MQ=255)                                                                                                                                                                                                 gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATg              >  1:301001/1‑241 (MQ=255)                                                                                                                                                                                                 gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGccat    >  1:238826/1‑248 (MQ=255)                                                                                                                                                                                                   tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAGGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTatga                                        >  1:437524/1‑213 (MQ=255)                                                                                                                                                                                                   tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTc                                                         >  1:647836/1‑196 (MQ=255)                                                                                                                                                                                                   tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTg  >  1:179552/1‑251 (MQ=255)                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                   AACGAACGCAAACGCCGGCAAGGCTTGGCGGGCGACAGTCGTTCTGTTGAAAACCAAGTGCAAAAGCAACGACAAACGATAGACGATGCGTTTCGCCGTTTTCACACGCAACGAGAATCTATTGAACGCCAAATTCGCGGCGAAAGCGAACCGCTCTATAAAAGCAATAAAAATATCAATGTAGACTGGATGGATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTG  >  1/728330‑728774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑