Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,817,233	2 bp→GG	intergenic (+35333/+18150)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,817,233	0	C	G	100.0%	83.9 / NA	31	intergenic (+35333/+18151)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/18);  total (13/18)
*	1	1,817,234	0	A	G	100.0%	87.6 / NA	31	intergenic (+35334/+18150)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/18);  total (13/18)

TAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTT  >  1/1816996‑1817469                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                            tgaaCGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCg                                                                                                                                                                                                                                   <  1:384749/248‑1 (MQ=255)               aCTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGt                                                                                                                                                                                                                    >  1:881958/1‑251 (MQ=255)                 tttAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTaa                                                                                                                                                                                                                  <  1:325185/251‑1 (MQ=255)                     gTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggg                                                                                                                                                                                                              >  1:612072/1‑249 (MQ=255)                      tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtg                                                                                                                                                                                                             <  1:891276/251‑4 (MQ=255)                      tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtg                                                                                                                                                                                                             <  1:861517/251‑4 (MQ=255)                      tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATgg                                                                                                                                                                                                               <  1:778134/249‑2 (MQ=255)                         aCGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgaga                                                                                                                                                                                                          <  1:603299/251‑7 (MQ=255)                          cGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaa                                                                                                                                                                                                         <  1:620902/251‑8 (MQ=255)                          cGGCAACGACGGTTTGCAACATGTGCTTATCGCGTTGGCGAGAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCAGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACTCTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaa                                                                                                                                                                                                         <  1:452723/251‑8 (MQ=255)                           ggCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaat                                                                                                                                                                                                        >  1:165049/1‑243 (MQ=255)                                     gtttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag                                                                                                                                                                                              >  1:52282/3‑233 (MQ=255)                                     gtttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCCACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag                                                                                                                                                                                              >  1:55082/3‑233 (MQ=255)                                      tttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag                                                                                                                                                                                              <  1:375722/249‑19 (MQ=255)                                             ccTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggagcggataa                                                                                                                                                                                       >  1:332391/1‑225 (MQ=255)                                             ccTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggagcggataa                                                                                                                                                                                       >  1:799382/1‑225 (MQ=255)                                                                       aCGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATg                                                                                                                                                                                                                         >  1:258650/1‑190 (MQ=255)                                                                                 gatgaAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAAt                                                                                                                                                                                                                 <  1:260690/188‑1 (MQ=255)                                                                                                                                                                 ggTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATgg                                                                                                                                                                                                               >  1:132008/1‑110 (MQ=255)                                                                                                                                                                                              caccggggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGt                                       <  1:429082/237‑1 (MQ=255)                                                                                                                                                                                                   gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGAt                                                                                                                                 >  1:572457/10‑156 (MQ=255)                                                                                                                                                                                                   gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTtat                                                                                          >  1:699651/10‑195 (MQ=255)                                                                                                                                                                                                     gaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAg                                 >  1:884751/8‑250 (MQ=255)                                                                                                                                                                                                      aaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGc                                <  1:207804/244‑1 (MQ=255)                                                                                                                                                                                                            aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                         <  1:296055/251‑1 (MQ=255)                                                                                                                                                                                                            aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                         <  1:443109/251‑1 (MQ=255)                                                                                                                                                                                                                  cTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTc                                                                                                    <  1:845446/170‑1 (MQ=255)                                                                                                                                                                                                                        caccagAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc             <  1:873801/246‑1 (MQ=255)                                                                                                                                                                                                                                   cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt   <  1:295554/251‑1 (MQ=255)                                                                                                                                                                                                                                   cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt   <  1:214057/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAttt  >  1:881110/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                            TAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTT  >  1/1816996‑1817469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑