Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	949,085	G→A	intergenic (‑24368/‑166023)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	949,085	0	G	A	100.0%	217.8 / NA	76	intergenic (‑24368/‑166023)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/36);  total (40/36)

CTTATCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGGCATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATCCAATG  >  1/948840‑949327                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   cTTATCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTa                                                                                                                                                                                                                                               >  1:584213/1‑251 (MQ=255)   tATCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACg                                                                                                                                                                                                                                             <  1:136589/251‑1 (MQ=255)    aTCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACg                                                                                                                                                                                                                                             >  1:593611/1‑250 (MQ=255)    aTCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACg                                                                                                                                                                                                                                             >  1:48649/1‑250 (MQ=255)    aTCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGc                                                                                                                                                                                                                                            <  1:102532/251‑1 (MQ=255)                       cGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTggg                                                                                                                                                                                                                          >  1:827022/1‑250 (MQ=255)                         aagaaataaatcaccacTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCaa                                                                                                                                                                                                                       >  1:214845/17‑251 (MQ=255)                           gaaataaatcaccacTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAAc                                                                                                                                                                                                                      >  1:738515/15‑250 (MQ=255)                             aCTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCctc                                                                                                                                                                                                                              >  1:144352/1‑240 (MQ=255)                                             ggCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTAAGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCtt                                                                                                                                                                                                   >  1:356756/1‑251 (MQ=255)                                             ggCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGCGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCtt                                                                                                                                                                                                   >  1:600782/1‑251 (MQ=255)                                              gCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCctc                                                                                                                                                                                                                              >  1:509198/1‑223 (MQ=255)                                              gCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGc                                                                                                                                                                                                                         >  1:192687/1‑228 (MQ=255)                                              gCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCg                                                                                                                                                                                                                    >  1:517724/1‑233 (MQ=255)                                                aTTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCt                                                                                                                                                                                                <  1:337953/251‑1 (MQ=255)                                                  tATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCTCATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTAt                                                                                                                                                                                              <  1:245083/251‑1 (MQ=255)                                                   aTGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGc                                                                                                                                                                                                                                            >  1:145439/1‑204 (MQ=255)                                                   aTGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCt                                                                                                                                                                                                    <  1:114072/244‑1 (MQ=255)                                                             atatTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTc                                                                                                                                                                                          >  1:481110/1‑244 (MQ=255)                                                              tatTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTaaaa                                                                                                                                                                                   >  1:377422/1‑250 (MQ=255)                                                              tatTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAAc                                                                                                                                                                                  <  1:369248/251‑1 (MQ=255)                                                                        gcagGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCa                                                                                                                                                                        >  1:811269/1‑251 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGc                                                                                                                                                                                                                                            >  1:200177/1‑180 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGc                                                                                                                                                                                                                         <  1:657284/199‑1 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTc                                                                                                                                                                                          <  1:614887/230‑1 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCttt                                                                                                                                                                                       >  1:486766/1‑233 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTg                                                                                                                                                                     >  1:708892/1‑251 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTg                                                                                                                                                                     >  1:776074/1‑251 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTg                                                                                                                                                                     >  1:608345/1‑251 (MQ=255)                                                                           ggCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTg                                                                                                                                                                     >  1:618465/1‑251 (MQ=255)                                                                             cTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCa                                                                                                                                                                   >  1:79377/1‑251 (MQ=255)                                                                             cTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCa                                                                                                                                                                   >  1:467698/1‑251 (MQ=255)                                                                              tACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCaa                                                                                                                                                                  <  1:1383/251‑1 (MQ=255)                                                                                       gttgttATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCctc                                                                                                                                                                                                                              <  1:761751/182‑1 (MQ=255)                                                                                        ttgttATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGa                                                                                                                                                        <  1:4108/251‑1 (MQ=255)                                                                                        ttgttATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGa                                                                                                                                                        >  1:863875/1‑251 (MQ=255)                                                                                                   gTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTgggg                                                                                                                                             >  1:625383/1‑251 (MQ=255)                                                                                                    tATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTgggg                                                                                                                                             <  1:391897/250‑1 (MQ=255)                                                                                                    tATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTgggg                                                                                                                                             <  1:495619/250‑1 (MQ=255)                                                                                                    tATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGAGTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTggggg                                                                                                                                            <  1:445581/251‑1 (MQ=255)                                                                                                          cacaTTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAg                                                                                                                                        <  1:361615/249‑1 (MQ=255)                                                                                                            caTTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCTGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTAt                                                                                                                                    >  1:751579/1‑251 (MQ=255)                                                                                                                 tATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGtt                                                                                                                               >  1:686340/1‑251 (MQ=255)                                                                                                                 tATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGtt                                                                                                                               >  1:182117/1‑251 (MQ=255)                                                                                                                  aTCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGttt                                                                                                                              <  1:428558/251‑1 (MQ=255)                                                                                                                        gCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAAc                                                                                                                                                                                  >  1:109079/1‑193 (MQ=255)                                                                                                                            tCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCg                                                                                                                    <  1:407504/251‑1 (MQ=255)                                                                                                                                aTGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGt                                                                                                                                       >  1:392523/1‑228 (MQ=255)                                                                                                                                      aaTTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGAc                                                                                                          <  1:268386/251‑1 (MQ=255)                                                                                                                                       aTTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGt                                                                                                                                <  1:512482/228‑1 (MQ=255)                                                                                                                                            aTGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGGTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGt                                                                                                    >  1:654070/1‑251 (MQ=255)                                                                                                                                                   ttcATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAAc                                                                                              <  1:59186/250‑1 (MQ=255)                                                                                                                                                         tggtggGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGa                                                                                                                                                        <  1:418410/186‑1 (MQ=255)                                                                                                                                                         tggtggGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGt                                                                                                                                >  1:200330/1‑210 (MQ=255)                                                                                                                                                              ggATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGtt                                                                                  >  1:540793/1‑251 (MQ=255)                                                                                                                                                                  tGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAAt                                                                              <  1:18638/251‑1 (MQ=255)                                                                                                                                                                   gCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTTGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATg                                                                             >  1:74266/1‑251 (MQ=255)                                                                                                                                                                    cTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATg                                                                             <  1:175004/250‑1 (MQ=255)                                                                                                                                                                    cTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATg                                                                             <  1:295170/250‑1 (MQ=255)                                                                                                                                                                    cTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGa                                                                            <  1:747295/251‑1 (MQ=255)                                                                                                                                                                                   gaTTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCa                                                             >  1:365565/1‑251 (MQ=255)                                                                                                                                                                                         ttCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAg                                                       <  1:651340/251‑1 (MQ=255)                                                                                                                                                                                                                        cACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAggg                        <  1:233926/251‑1 (MQ=255)                                                                                                                                                                                                                        cACGACGTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAggg                        <  1:507412/251‑1 (MQ=255)                                                                                                                                                                                                                          cgacgTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAggg                        <  1:892591/249‑1 (MQ=255)                                                                                                                                                                                                                            acgTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTg                                                                                                                                 <  1:787947/142‑1 (MQ=255)                                                                                                                                                                                                                            acgTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGATGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCATTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGGGCCAAAAGTGAGTTACTGAAAGGGTTAt                    >  1:438960/1‑251 (MQ=255)                                                                                                                                                                                                                              gTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTg                  >  1:745805/1‑251 (MQ=255)                                                                                                                                                                                                                              gTACCGCTATTTACGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTg                  >  1:500234/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATcc      <  1:675425/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATcc      <  1:5958/251‑1 (MQ=255)                                                                                                                                                                                                                                           cGGCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATCCa     <  1:808774/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCGTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATCCaa    <  1:62716/250‑1 (MQ=255)                                                                                                                                                                                                                                               gTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTg                                                                                                                                 <  1:29723/123‑1 (MQ=255)                                                                                                                                                                                                                                               gTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAAt                                                                              <  1:469675/174‑1 (MQ=255)                                                                                                                                                                                                                                               gTTATGGACATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATCCAATg  >  1:355990/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   CTTATCCTATTCGCTATTTTGACGAAAAACTCACCAACGGCTTTGGCATTATGGTGGACGATATTTTGGCAGCAGGCTACAGCATCGTTGTTATTTTTGTATTAGCACATTTTATCTAGGCAATCAGATGTTAAATTTAATGTTCGTTCATTTGGTGGGATTGCTTTCCCCCGGACCGGATTTTTTCTATGTGAGCCGTGTCGCGGCAATCCGTTCACGACGTACCGCTATTTACGGCGTTATGGGCATTACGCTCGGCGTGTTCCTCTGGGCAACCGCTGCCGTGTTGGGGCTTGCTATCGTCTTTAAAACCTCGCCGGCATTGCAAGGCGTGGTGATGGCATTGGGGGGCAGTTATTTGTTTTATCTTGGCGTGAAAATGACGCGCGTTAAAACCAATGCGGTGTTTAATGATTCGGCGGAAGGGCAAAAAAGCGTGGGTGCCAAAAGTGAGTTACTGAAAGGGTTATTGGTCAATTTATCCAATG  >  1/948840‑949327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑