Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	935,810	A→G	intergenic (‑11093/‑179298)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	935,810	0	A	G	98.6%	203.0 / NA	73	intergenic (‑11093/‑179298)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (31/41);  minor base T (0/1);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTATCAACAACCACGATTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATATTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACTTTGCCCATTTACGGCGT  >  1/935573‑936055                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gATTATCAACAACCACGATTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAAtttt                                                                                                                                                                                                                                          >  1:284962/1‑251 (MQ=255) gATTATCAACAACCACGATTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAAtttt                                                                                                                                                                                                                                          >  1:32325/1‑251 (MQ=255)             ccACGATTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCAt                                                                                                                                                                                                                              <  1:832745/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:893500/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:820711/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:807572/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:688391/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:575457/251‑1 (MQ=255)                  aTTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         <  1:35223/251‑1 (MQ=255)                     ccTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATaa                                                                                                                                                                                                                       >  1:776774/1‑250 (MQ=255)                     ccTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATaa                                                                                                                                                                                                                       >  1:256056/1‑250 (MQ=255)                      cTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAAcc                                                                                                                                                                                                                     <  1:495958/251‑1 (MQ=255)                       tttACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCt                                                                                                                                                                                                                    <  1:92548/251‑1 (MQ=255)                       tttACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCt                                                                                                                                                                                                                    <  1:31728/251‑1 (MQ=255)                          aCTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTtg                                                                                                                                                                                                                                                    >  1:277615/1‑216 (MQ=255)                          aCTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCt                                                                                                                                                                                                                    >  1:138277/1‑248 (MQ=255)                                     aGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         >  1:137669/1‑232 (MQ=255)                                         gATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATtatat                                                                                                                                                                                                                         >  1:664013/1‑228 (MQ=255)                                         gATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACgg                                                                                                                                                                                                  >  1:743490/1‑251 (MQ=255)                                         gATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACgg                                                                                                                                                                                                  >  1:257052/1‑251 (MQ=255)                                          aTTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAAc                                                                                                                                                                                                                      <  1:785548/230‑1 (MQ=255)                                                      gTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCAt                                                                                                                                                                                                                              <  1:381652/210‑1 (MQ=255)                                                       tttGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCtgt                                                                                                                                                                                    <  1:594426/251‑1 (MQ=255)                                                               tGCCGTTTCGGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCt                                                                                                                                                                             <  1:217427/250‑1 (MQ=255)                                                               tGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCt                                                                                                                                                                             <  1:482099/250‑1 (MQ=255)                                                                   gTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCCCCCCGCCACGGGCAGGCAAAGCTGTTGTAt                                                                                                                                                                               <  1:893195/244‑1 (MQ=255)                                                                                   gtgGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGc                                                                                                                                                                                       <  1:163205/220‑1 (MQ=255)                                                                                    tgGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTAtt                                                                                                                                                        <  1:880371/250‑1 (MQ=255)                                                                                              ggATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTg                                                                                                                                              >  1:4152/1‑250 (MQ=255)                                                                                                           gTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAAc                                                                                                                                                 <  1:142051/234‑1 (MQ=255)                                                                                                                        cccATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATAc                                                                                                                   <  1:174755/251‑1 (MQ=255)                                                                                                                        cccATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTtata                                                                                                                    >  1:775372/1‑250 (MQ=255)                                                                                                                        cccATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATAc                                                                                                                   <  1:258799/251‑1 (MQ=255)                                                                                                                         ccATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATAcc                                                                                                                  <  1:848967/251‑1 (MQ=255)                                                                                                                         ccATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATAcc                                                                                                                  <  1:246301/251‑1 (MQ=255)                                                                                                                           aTTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCAt                                                                                                                >  1:882427/1‑251 (MQ=255)                                                                                                                              tGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGAAAATCCATTTTCGGCGTGGGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATAc                                                                                                                   <  1:673402/245‑1 (MQ=255)                                                                                                                                  tATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTggg                                                                                                         >  1:820224/1‑251 (MQ=255)                                                                                                                                   ttttCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGc                                                                                                        <  1:357292/250‑1 (MQ=255)                                                                                                                                   aTTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGc                                                                                                        <  1:348375/251‑1 (MQ=255)                                                                                                                                             gCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGggc                                                                                                                                                                                                >  1:553600/1‑153 (MQ=255)                                                                                                                                                ccGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGt                                                                                                                         >  1:853064/1‑221 (MQ=255)                                                                                                                                                ccGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCg                                                                                           >  1:314454/1‑251 (MQ=255)                                                                                                                                                 cGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCg                                                                                           >  1:42098/1‑250 (MQ=255)                                                                                                                                                  gATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCt                                                                                                                                                                             <  1:868156/167‑1 (MQ=255)                                                                                                                                                    ttggCCGCGTGGGTTTCGGAAATTATTTGCCTTGTTGGAGCGTTATCGCCGGTGCAAGTCCATTTTGGGTGTGTGTTAAGGACGTCAAGTTTTGTGCGAATTTTTCGGTGCGGCGTTATGTAACCTGCGGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAGCTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCAtt                                                                                       <  1:290551/248‑1 (MQ=255)                                                                                                                                                             tgcgtATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACg                                                                               >  1:368895/1‑250 (MQ=255)                                                                                                                                                                     cGCAATTTTTTGCTTTGTTGGAACGTTATCACCGGTGCAAATCCTTTTTCGGCGTGTGTTTAGGCCATCAAATGTTGTGCGAATTTTTCGGTGCGGCTTTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAg                                                                      <  1:452378/251‑1 (MQ=255)                                                                                                                                                                        aaTTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCATTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGAtt                                                                   <  1:316018/251‑1 (MQ=255)                                                                                                                                                                         attattTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCaaa                                                                                                                              <  1:26983/191‑1 (MQ=255)                                                                                                                                                                         attattTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAGATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTa                                                                  >  1:409501/1‑251 (MQ=255)                                                                                                                                                                         attattTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTa                                                                  >  1:101564/1‑251 (MQ=255)                                                                                                                                                                          ttattTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCt                                                                                                                                                                                                                    <  1:766013/104‑1 (MQ=255)                                                                                                                                                                          ttattTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTa                                                                  >  1:764680/1‑250 (MQ=255)                                                                                                                                                                                     gttgGAACGTTATCACCGGTGCAAATCCATTTTCTGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCCGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTa                                                      >  1:746190/1‑251 (MQ=255)                                                                                                                                                                                     gttgGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGAt                                                                    <  1:58624/237‑1 (MQ=255)                                                                                                                                                                                     gttgGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTAc                                                                 <  1:494007/240‑1 (MQ=255)                                                                                                                                                                                      ttgGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAg                                                     <  1:703608/251‑1 (MQ=255)                                                                                                                                                                                             gTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATaa                                               >  1:611075/1‑250 (MQ=255)                                                                                                                                                                                             gTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATaa                                               >  1:538147/1‑250 (MQ=255)                                                                                                                                                                                              ttATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAAc                                              <  1:381639/250‑1 (MQ=255)                                                                                                                                                                                                  cACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGccc                                                                                   >  1:236540/1‑209 (MQ=255)                                                                                                                                                                                                       gTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAAcc                                                                                            <  1:148769/195‑1 (MQ=255)                                                                                                                                                                                                        tGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGc                                   <  1:259876/251‑1 (MQ=255)                                                                                                                                                                                                                        gCGTTTGTTTAGGCCATCAAATGTTGGGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAAc                    <  1:432918/250‑1 (MQ=255)                                                                                                                                                                                                                         cGTGTGTTTAGGACATCAAATGTTGGGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCCCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACtt                  <  1:34480/251‑1 (MQ=255)                                                                                                                                                                                                                              gtTTAGGACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGAtt                                                                   >  1:339610/1‑197 (MQ=255)                                                                                                                                                                                                                              gtTTAGGACATAAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCg                                                                                                <  1:331805/168‑1 (MQ=255)                                                                                                                                                                                                                                   ggACATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACTTTGCCCATTTa        >  1:88160/1‑251 (MQ=255)                                                                                                                                                                                                                                      cATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATACCCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGcc                                                             <  1:802901/195‑1 (MQ=255)                                                                                                                                                                                                                                      cATCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCt                                                                                                                                                                             >  1:253569/1‑83 (MQ=255)                                                                                                                                                                                                                                       aTCAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACTTTGCCCATTTACgg     >  1:682397/1‑250 (MQ=255)                                                                                                                                                                                                                                         cAAATGTTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACTTTGCCCATTTACGGCGt  >  1:548959/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GATTATCAACAACCACGATTCCTTTACTTTCAATTTAGTGGATTTAATCCGCCGTTTGGCAGTGCCGTTTCAGGTGGTGAATGTGGAAGACCTGGATTTGGACGCGGTGGAAAATTTCTCCCATTTGCTTATTTCTCCCGGCCCCGATGTGCCGCGTGCGTATCCGCAATTATTTGCCTTGTTGGAACGTTATCACCGGTGCAAATCCATTTTCGGCGTGTGTTTAGGACATCAAATATTGTGCGAATTTTTCGGTGCGGCATTATATAACCTGCCGTCGCCCCGCCACGGGCAGGCAAAGCTGTTGTATCTTGTGTCGGATTCGCCGTTATTCAAAAACTTGCCGCCAAGCTTTCAAATCGGTTTATACCATTCCTGGGCGGTGGGCGAACCGCATTGCCCCACGCAACTACAGATTACTGCCCGTTGTAGCGATAACATCATCATGGCAATGCAGCACAAAACTTTGCCCATTTACGGCGT  >  1/935573‑936055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑