Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	924,784	T→G	intergenic (‑67/‑190324)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	924,784	0	T	G	100.0%	194.1 / NA	66	intergenic (‑67/‑190324)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/38);  total (28/38)

GTCAGTTTGTATTGAAAAACACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGTAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAACAGGAAG  >  1/924538‑925023                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gcatgTTTGTATTGAAAAACACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGaaa                                                                                                                                                                                                                                              <  1:478102/246‑1 (MQ=255)         gTATTGAAAAACACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTAcgcg                                                                                                                                                                                                                                       >  1:157145/1‑249 (MQ=255)         gTATTGAAAAACACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTAcgcg                                                                                                                                                                                                                                       >  1:283994/1‑249 (MQ=255)                aaaaCACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtat                                                                                                                                                                                                                              <  1:785738/251‑1 (MQ=255)                 aaaCACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtata                                                                                                                                                                                                                             <  1:618849/251‑1 (MQ=255)                  aaCACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            <  1:333843/251‑1 (MQ=255)                    cacCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTatatat                                                                                                                                                                                                                          <  1:850251/251‑1 (MQ=255)                          gAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTcgac                                                                                                                                                                                                                     <  1:354978/250‑1 (MQ=255)                                   ccGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTcc                                                                                                                                                                                                           <  1:358705/251‑1 (MQ=255)                                               tctcAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCaaaaaa                                                                                                                                                                                               >  1:123340/1‑251 (MQ=255)                                                    aGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCt                                                                                                                                                                                                       <  1:775240/238‑1 (MQ=255)                                                      gTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            >  1:275330/1‑215 (MQ=255)                                                       tCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACTCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACg                                                                                                                                                                                       <  1:425342/251‑1 (MQ=255)                                                            cacaGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGataat                                                                                                                                                                                  <  1:781264/251‑1 (MQ=255)                                                                gAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatata                                                                                                                                                                                                                           >  1:223118/1‑206 (MQ=255)                                                                gAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            >  1:592536/1‑205 (MQ=255)                                                                gAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAgg                                                                                                                                                                               >  1:221540/1‑250 (MQ=255)                                                                gAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAgg                                                                                                                                                                               >  1:287390/1‑250 (MQ=255)                                                                 aaTAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGc                                                                                                                                                                              <  1:227610/250‑1 (MQ=255)                                                                            ccTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGAt                                                                                                                                                                  <  1:402501/251‑1 (MQ=255)                                                                            ccTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGAt                                                                                                                                                                  <  1:81596/251‑1 (MQ=255)                                                                              tCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAg                                                                                                                                                                <  1:834407/251‑1 (MQ=255)                                                                                gCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            >  1:156332/1‑189 (MQ=255)                                                                                gCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTcc                                                                                                                                                                                                           <  1:564665/206‑1 (MQ=255)                                                                                        gCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGa                                                                                                                                                        <  1:322852/249‑1 (MQ=255)                                                                                        gCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGa                                                                                                                                                        <  1:510708/249‑1 (MQ=255)                                                                                           aGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAatat                                                                                                                                                    <  1:576424/250‑1 (MQ=255)                                                                                                  gTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGc                                                                                                                                                                              >  1:268800/1‑217 (MQ=255)                                                                                                     aaTTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAgcgc                                                                                                                                          >  1:746212/1‑250 (MQ=255)                                                                                                                ggAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTAt                                                                                                                               <  1:221016/250‑1 (MQ=255)                                                                                                                         gCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCttatt                                                                                                                     >  1:837312/1‑251 (MQ=255)                                                                                                                               aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGcc                                                                                                               <  1:513260/251‑1 (MQ=255)                                                                                                                                      aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGc                                                                                                                <  1:414557/243‑1 (MQ=255)                                                                                                                                      aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTaaa                                                                                                        >  1:480261/1‑251 (MQ=255)                                                                                                                                                tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGt                                                                                               >  1:852989/1‑250 (MQ=255)                                                                                                                                                       gTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtat                                                                                                                                                                                                                              >  1:448788/1‑116 (MQ=255)                                                                                                                                                       gTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATg                                                                                                                                    >  1:55243/1‑206 (MQ=255)                                                                                                                                                       gTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAgcg                                                                                       >  1:380452/1‑251 (MQ=255)                                                                                                                                                               ctACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAatat                                                                                                                                                    >  1:544156/1‑182 (MQ=255)                                                                                                                                                                        gAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAatat                                                                                                                                                    <  1:696082/173‑1 (MQ=255)                                                                                                                                                                           cTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTaa                                                                     <  1:337897/249‑1 (MQ=255)                                                                                                                                                                             aTTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAg                                                                                                                                                                >  1:650266/1‑156 (MQ=255)                                                                                                                                                                               tCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTg                                                                 >  1:423280/1‑249 (MQ=255)                                                                                                                                                                                          caTAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGt                                                    <  1:211203/251‑1 (MQ=255)                                                                                                                                                                                                            gCATTATACGAATATTTTTGTGCAGTGCAAGTATACAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTc                                                         >  1:614393/1‑228 (MQ=255)                                                                                                                                                                                                            gCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAg                                                                                                                                                                <  1:101315/125‑1 (MQ=255)                                                                                                                                                                                                            gCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAg                                   <  1:867970/250‑1 (MQ=255)                                                                                                                                                                                                            gCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGt                                  >  1:464831/1‑251 (MQ=255)                                                                                                                                                                                                             cATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTAGCGATAATGAAAATAGt                                  <  1:44989/250‑1 (MQ=255)                                                                                                                                                                                                                 ataCGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            <  1:158434/60‑1 (MQ=255)                                                                                                                                                                                                                         atTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTcc                                                                                                  <  1:630288/174‑1 (MQ=255)                                                                                                                                                                                                                         atTTTTGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAgg                     >  1:593461/1‑251 (MQ=255)                                                                                                                                                                                                                          tttttGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTAttt                                                                                                                             <  1:523959/146‑1 (MQ=255)                                                                                                                                                                                                                          tttttGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGt                                  <  1:129522/237‑1 (MQ=255)                                                                                                                                                                                                                          tttttGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGc                              >  1:873773/1‑241 (MQ=255)                                                                                                                                                                                                                           ttttGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCa                   <  1:22736/251‑1 (MQ=255)                                                                                                                                                                                                                            tttGTGCAGTGCAAGTATAAAATCGTGGAAATTACGCGCTTGTTtatat                                                                                                                                                                                                                            <  1:713209/49‑1 (MQ=255)                                                                                                                                                                                                                               gtgcagtgcaAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATg                                                                                                     <  1:98007/165‑1 (MQ=255)                                                                                                                                                                                                                                    gtgcaAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTAt                                                                                                                               >  1:330876/1‑134 (MQ=255)                                                                                                                                                                                                                                    gtgcaAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGc                                                                                                                          <  1:816984/139‑1 (MQ=255)                                                                                                                                                                                                                                    gtgcaAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAg                                   >  1:148358/1‑226 (MQ=255)                                                                                                                                                                                                                                       caAGTATAAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAAc        <  1:834523/250‑1 (MQ=255)                                                                                                                                                                                                                                            ataAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAACAGGAAg  >  1:801227/1‑251 (MQ=255)                                                                                                                                                                                                                                            ataAAATCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAACAGGAAg  <  1:405479/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAAc        >  1:31277/1‑240 (MQ=255)                                                                                                                                                                                                                                                 aTCGTGGAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAAc        <  1:437217/240‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GTCAGTTTGTATTGAAAAACACCGGGAATTCTGACCGACTTTTCAGTCTCAAGGTCGCGCACAGAATAAAAAATCCCTCGCTGAAAGGCGAGGGATTGTAAATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTTCACATAAAACGAATGAGACGGCATTATACGAATATTTTTGTGCAGTGCAAGTATAAAATCGTGTAAATTACGCGCTTGTTTATATATTCGACGATTTAGTCCATCTTCAAAAAATGTTCACGATAATAGGCTAACTCTTTGATAGATTCACGAATATCGTCCAGCGCCAAATGCGTATTTTGCTTATTAAAGCCGTTTAAAATGTCCGGTCTCCAGCGGGCGGCAAGTTCTTTTAAGGTGCTGACGTCAAGGTGGCGATAATGAAAATAGTCGGCAAGTTCAGGCATGTATTTAAACAGGAAG  >  1/924538‑925023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑