Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	879,065	A→G	intergenic (‑8746/‑17916)	unknown ← / → unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	879,065	0	A	G	100.0%	127.7 / NA	43	intergenic (‑8746/‑17916)	unknown/unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

CTATTGATTCTGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAAAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGAAAAAGGCCAGCTGAAATT  >  1/878822‑879305                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 cTATTGATTCTGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGaaaa                                                                                                                                                                                                                                           >  1:582676/1‑251 (MQ=255)  tATTGATTCTGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGaaaaa                                                                                                                                                                                                                                          <  1:182445/251‑1 (MQ=255)    ttGATTCTGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATc                                                                                                                                                                                                                                        <  1:455554/251‑1 (MQ=255)           tGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATc                                                                                                                                                                                                                                 <  1:547270/251‑1 (MQ=255)            gCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAAt                                                                                                                                                                                                                                  >  1:685685/1‑249 (MQ=255)                     cACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCa                                                                                                                                                                                                                       <  1:99095/251‑1 (MQ=255)                                             cAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATc                                                                                                                                                                                                                        >  1:539969/1‑226 (MQ=255)                                              aattaattCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATc                                                                                                                                                                                                                        <  1:64919/225‑1 (MQ=255)                                                   attCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGa                                                                                                                                                                                         <  1:428446/251‑1 (MQ=255)                                                        ggTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCtt                                                                                                                                                                                    >  1:174657/1‑251 (MQ=255)                                                        ggTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCtt                                                                                                                                                                                    >  1:458033/1‑251 (MQ=255)                                                                        gcgAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATgg                                                                                                                                                                     <  1:203671/250‑1 (MQ=255)                                                                                       gaagaaTTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTc                                                                                                                                                                                                             >  1:741444/1‑195 (MQ=255)                                                                                        aagaaTTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTg                                                                                                                                                    <  1:660737/251‑1 (MQ=255)                                                                                             tttGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAg                                                                                                                                               <  1:56937/251‑1 (MQ=255)                                                                                                           aTTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATc                                                                                                                                                                                                                        >  1:723065/1‑164 (MQ=255)                                                                                                           aTTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATagagag                                                                                                                                  >  1:583510/1‑250 (MQ=255)                                                                                                           aTTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATGGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGagc                                                                                                                                 <  1:439756/251‑1 (MQ=255)                                                                                                                     gCGAAGAAATTCCTTGATACTCAATCGCAATTTGTGTTTAGTCCGACATTAAATAATCAAGCCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGaa                                                                                                                       >  1:800140/1‑251 (MQ=255)                                                                                                                        aagaaATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGGTGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAAt                                                                                                                    >  1:438356/1‑251 (MQ=255)                                                                                                                        aagaaATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAAt                                                                                                                    >  1:501407/1‑251 (MQ=255)                                                                                                                                ccTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCa                                                                                                                                                                                                                       >  1:549106/1‑144 (MQ=255)                                                                                                                                ccTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATagagag                                                                                                                                  >  1:260170/1‑229 (MQ=255)                                                                                                                                         tCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGtga                                                                                                   <  1:692513/251‑1 (MQ=255)                                                                                                                                                cAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGaaa                                                                                                                      >  1:817666/1‑225 (MQ=255)                                                                                                                                                   tttGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGgtgt                                                                                         >  1:395306/1‑251 (MQ=255)                                                                                                                                                                  aCATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGc                                                                                                                                                                                      >  1:747770/1‑143 (MQ=255)                                                                                                                                                                      tAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCaa                                                                      <  1:866301/251‑1 (MQ=255)                                                                                                                                                                         cTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGtgaatga                                                                                               >  1:149415/1‑223 (MQ=255)                                                                                                                                                                           aaTCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCg                                                                                                                                                                   <  1:861241/153‑1 (MQ=255)                                                                                                                                                                                  tCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGt                                                           <  1:243830/250‑1 (MQ=255)                                                                                                                                                                                   cGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTgg                                                            >  1:464012/1‑248 (MQ=255)                                                                                                                                                                                        aaaTTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGAc                                                    <  1:786655/251‑1 (MQ=255)                                                                                                                                                                                                   aaTTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGtgaatga                                                                                               <  1:98839/197‑1 (MQ=255)                                                                                                                                                                                                             tttCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATagag                                                                                                                                    <  1:848467/150‑1 (MQ=255)                                                                                                                                                                                                             tttCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCGGCCACACAAGtg                                                                                                    >  1:451667/1‑182 (MQ=255)                                                                                                                                                                                                             tttCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGAt                               >  1:698526/1‑251 (MQ=255)                                                                                                                                                                                                             tttCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGAt                               >  1:403173/1‑251 (MQ=255)                                                                                                                                                                                                                  aTTGCAAGCTCTGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGt                          >  1:341531/1‑251 (MQ=255)                                                                                                                                                                                                                          ctctGATTTTGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGaa                   <  1:508412/250‑1 (MQ=255)                                                                                                                                                                                                                                   tGAAAATTCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGAAAAAGGCCAg          >  1:637410/1‑250 (MQ=255)                                                                                                                                                                                                                                         ttCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGAAAAAGGCCAGCTGAAAt   <  1:240012/251‑1 (MQ=255)                                                                                                                                                                                                                                          tCTATTAGCAGAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGAAAAAGGCCAGCTGAAAtt  >  1:820154/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 CTATTGATTCTGCTTTTAATCACGTTGACGGTGAAACTTTTAATCAATTAATTCAGGTTTATACCGAGGCGGCGAAAAAGCAGCAAGAAGAATTTGCCGAAGCCAAATTACAGGCAGCGAAGAAATTCCTTGATACTCAACCGCAATTTGTGTTTAGTCCGACATTAACTAATCAAGTCGGTAAAATTGCCTCAAATTTAGATATTTCTATTGCAAGCTCTGATTTTGAAAATTCTATTAGCAAAGGAAAAATCTTCAATCTATTTAATCACTTTATTTTCAAAGTGGATGCGAACAAAGATGCTTTAATCGAAGAAATGGCGAACGCACTGCAATTGAAAAGCCCGGATAGAGAGCAAGCAATGAAAATGGCAGCCACACAAGTGAATGAAGGTGTGAAAAGCGGTGTTCTGCAAGGTGTATTGGTCGAAGACGGTAAACAAGTAAAATTGGATCTCGTATTGGAAAAAGGCCAGCTGAAATT  >  1/878822‑879305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑