Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	864,462	T→C	intergenic (+47264/+5566)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	864,462	0	T	C	100.0%	208.8 / NA	71	intergenic (+47264/+5566)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/27);  total (44/27)

GAAACGGTGCGCCCGGAAGTAGAAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCGGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGATACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGTATTCCCAC  >  1/864217‑864704                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   gAAACGGTGCGCCCGGAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTg                                                                                                                                                                                                                                                >  1:81857/1‑250 (MQ=255)        agccggcGGAATTAGCAGCCTGTTAAAAGCGGGGCGGCACCTACCAAAATCATATGTTCCCCCCCCTTTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGTTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTAATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTaa                                                                                                                                                                                                                                          <  1:788982/243‑1 (MQ=255)                gAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGc                                                                                                                                                                                                                                  >  1:202142/1‑249 (MQ=255)                gAAGTAGCAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGc                                                                                                                                                                                                                                  >  1:651444/1‑249 (MQ=255)                       caGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGTTCACCCCACGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTtctc                                                                                                                                                                                                                           <  1:358036/248‑1 (MQ=255)                             ggTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTCGATTATTTCACCGCAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGACAGCCTTTCTCTGGAAGa                                                                                                                                                                                                                    >  1:466103/1‑250 (MQ=255)                             ggTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCAAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGa                                                                                                                                                                                                                    >  1:464867/1‑250 (MQ=255)                                    gCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTg                                                                                                                                                                                                            >  1:109581/1‑251 (MQ=255)                                       ggTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGttt                                                                                                                                                                                                         >  1:531167/1‑251 (MQ=255)                                                 tATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                                                                                                                 >  1:668879/1‑249 (MQ=255)                                                  aTCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                                                                                                                 >  1:835888/1‑248 (MQ=255)                                                         caatcTGGTCTACCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAACCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAAcc                                                                                                                                                                                       <  1:493477/249‑1 (MQ=255)                                                               gttcaccccgcgttttACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                                                                                                                  <  1:174325/237‑1 (MQ=255)                                                                   accccACGCTTTCCCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGt                                                                                                                                                                             <  1:43364/249‑1 (MQ=255)                                                                    tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAAc                                                                                                                                                                                                                                        >  1:156010/1‑191 (MQ=255)                                                                    tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTa                                                                                                                                                                            >  1:726729/1‑251 (MQ=255)                                                                    tCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTa                                                                                                                                                                            >  1:654217/1‑251 (MQ=255)                                                                         cgttttccccGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                                                                                                                  <  1:781859/233‑1 (MQ=255)                                                                                cccGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtt                                                                                                                                                                >  1:87815/1‑251 (MQ=255)                                                                                   gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCCCTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttt                                                                                                                                                              >  1:437102/1‑250 (MQ=255)                                                                                   gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTaa                                                                                                                                                                                                                                          <  1:12120/174‑1 (MQ=255)                                                                                   gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATcc                                                                                                                                                                                                >  1:636353/1‑216 (MQ=255)                                                                                   gCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttt                                                                                                                                                              >  1:575899/1‑250 (MQ=255)                                                                                    ccTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGtttcc                                                                                                                                                             >  1:809456/1‑248 (MQ=255)                                                                                       aGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCccg                                                                                                                                                          >  1:641911/1‑250 (MQ=255)                                                                                                 gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                                                                                                               >  1:338978/1‑251 (MQ=255)                                                                                                 gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                                                                                                               >  1:658903/1‑251 (MQ=255)                                                                                                 gAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCaa                                                                                                                                               >  1:790972/1‑251 (MQ=255)                                                                                                         cACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCAcc                                                                                                                                                                                  <  1:330284/208‑1 (MQ=255)                                                                                                          cccaaaaGTGCGGTAGATTATTTCACCAAAAATAAAGAAAAACCCATCAGTGATTTTGCCGAAAATCTTGCGAATCCCGCCCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTAtgt                                                                                                                                      <  1:563536/247‑1 (MQ=255)                                                                                                          aCCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTAtgt                                                                                                                                      >  1:478385/1‑251 (MQ=255)                                                                                                                  tGCGGTAGATTATTCCCCCGAAAAAAAAGAAAAACCCACCAGTGATTTTGCCGAAAATCTGGCGAATACCGACACTTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAAc                                                                                                                                <  1:613537/249‑1 (MQ=255)                                                                                                                  ggCGGTAGATTATTCCCCCGAAAAAAAAAAAAAACCCATCAGTAATTTTGCCAAAAATCTTGCAAATCCCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAAc                                                                                                                                <  1:335059/248‑1 (MQ=255)                                                                                                                   gCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATc                                                                                                                                                                                                 >  1:443935/1‑183 (MQ=255)                                                                                                                      gTAGATTATTTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAAc                                                                                                                                                                                                                                        >  1:114565/1‑141 (MQ=255)                                                                                                                       tAGATTATTTCCCCGAAAATAAAAAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTcc                                                                                                                         <  1:865371/251‑1 (MQ=255)                                                                                                                          attattTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGt                                                                                                                      >  1:313483/1‑251 (MQ=255)                                                                                                                           ttattTCACCGAAAATAAAGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGtt                                                                                                                     >  1:814518/1‑251 (MQ=255)                                                                                                                                       aaaTAAAAAAAAACCCATCAGTGATTTTGCCAAAATTTTTGCGAATCCCGACCCCTATTCCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGc                                                                                                         <  1:698539/251‑1 (MQ=255)                                                                                                                                             aGAAAAATCCATCAGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTg                                                                                                                                                                                >  1:514423/1‑174 (MQ=255)                                                                                                                                                    tccatcagggtttttGCCGAAAATCTTGCGAATACCGACACCTTTTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGa                                                                                            <  1:735408/240‑1 (MQ=255)                                                                                                                                                    cccATCAGTGTTTTTGCCGAAAATCTTGCAAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGa                                                                                            <  1:106372/250‑1 (MQ=255)                                                                                                                                                                   gCCGAAAATCTTGCGAATACCGACCCCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAg                                                                                                                                                                        <  1:505452/160‑1 (MQ=255)                                                                                                                                                                    ccaaaaaTCTTGCGAATACCGCCACCTATTCCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACg                                                                            <  1:734494/248‑1 (MQ=255)                                                                                                                                                                              tGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGaaaa                                                                  >  1:248357/1‑251 (MQ=255)                                                                                                                                                                                          cacCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGCTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAAccc                                                       >  1:802627/1‑250 (MQ=255)                                                                                                                                                                                            ccTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCAtt                                                    >  1:738913/1‑251 (MQ=255)                                                                                                                                                                                               aTTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGCAAAATATGAAACCCATTtgt                                                 >  1:413088/1‑251 (MQ=255)                                                                                                                                                                                                         ccTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTaa                                       >  1:106812/1‑251 (MQ=255)                                                                                                                                                                                                           tCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCCCCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAgc                                     <  1:134943/251‑1 (MQ=255)                                                                                                                                                                                                           tCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCCCCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAgc                                     <  1:493791/251‑1 (MQ=255)                                                                                                                                                                                                            cGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTgtgt                                               >  1:539144/1‑240 (MQ=255)                                                                                                                                                                                                             gCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGa                                                                                            >  1:788572/1‑194 (MQ=255)                                                                                                                                                                                                                   gACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTgg                                                                                                                                    >  1:399742/1‑148 (MQ=255)                                                                                                                                                                                                                   gACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCggg                             >  1:189143/1‑251 (MQ=255)                                                                                                                                                                                                                   gACTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCggg                             >  1:288600/1‑251 (MQ=255)                                                                                                                                                                                                                    aCTTAGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCggg                             >  1:280138/1‑250 (MQ=255)                                                                                                                                                                                                                        aGTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGa                                                                                            >  1:7644/1‑183 (MQ=255)                                                                                                                                                                                                                         gTGCAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGTGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAg                       >  1:484030/1‑251 (MQ=255)                                                                                                                                                                                                                            cAACTTGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAgcgc                    <  1:311368/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGCACATTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGaaa               >  1:207885/1‑251 (MQ=255)                                                                                                                                                                                                                                   cacaTTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTCGAAGACATTCTTGTTTTCCCAATCCTGCGCACCCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAg              <  1:122852/250‑1 (MQ=255)                                                                                                                                                                                                                                     caTTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTgg           >  1:750725/1‑251 (MQ=255)                                                                                                                                                                                                                                     caTTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTgg           >  1:23818/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTTTAATCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGt          >  1:737352/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaTCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGTATTc      <  1:539164/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTCCAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGTATTcc     <  1:87579/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAg                                                                                       <  1:627275/166‑1 (MQ=255)                                                                                                                                                                                                                                              cAGTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAgc                                     <  1:48343/216‑1 (MQ=255)                                                                                                                                                                                                                                                gTCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGAt                                                                                           <  1:370710/160‑1 (MQ=255)                                                                                                                                                                                                                                                 tCGGACACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGCCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGTATTCCCAc  <  1:26163/248‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   GAAACGGTGCGCCCGGAAGTAGAAGCCTGGTTAAAGCAGGTCGGCAGCTATCAAAATCATCTGGTCATCCCACGCTTTACCCGCCTAGGGCTGCCTGAATATGCCACCCAAAGTGCGGTAGATTATTTCACCGAAAATAAAGAAAAATCCATCGGTGATTTTGCCGAAAATCTTGCGAATACCGACACCTATTTCGCCCGCCTCGCCCCTGACTTAGTGCAACTTGCACATTTAATCCAGTCGGATACTGCACTAAACGGCAGCCTTTCTCTGGAAGACATTCTTGTTTTCCCAATCCTGCGCAACCTCACCTGCGTAAAAGGCATCGTTTTCCCGCCGAAAGTCAACGCCTATGTGGAAACCATGTCCCGTTTGAGCCAAGTGCCGTTGTATTGGGATAAAGCGATTTAAACGAGGAGGAAAAATATGAAACCCATTTGTGTCGTCCTAAGCGGCGCGGGCATCAGCGCTGAAAGTGGTATTCCCAC  >  1/864217‑864704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑