Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 862,433 T→C intergenic (+45235/+7595) unknown → / ← unknown tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1862,4330TC100.0% 242.6 / NA 83intergenic (+45235/+7595)unknown/unknowntRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/40);  total (43/40)

ATGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTTGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTCC  >  1/862197‑862672
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
aTGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTa                                                                                                                                                                                                                                   <  1:593569/251‑1 (MQ=255)
        gaTATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAAc                                                                                                                                                                                                                              <  1:544668/248‑1 (MQ=255)
             aaataaCAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTatat                                                                                                                                                                                                                      <  1:6119/251‑1 (MQ=255)
                       tgtgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACG‑AAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTa                                                                                                                                                                                                           >  1:350751/1‑251 (MQ=255)
                         tgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTa                                                                                                                                                                                                                    >  1:844757/1‑241 (MQ=255)
                         tgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTa                                                                                                                                                                                                           >  1:263969/1‑250 (MQ=255)
                               ttgttgGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:730836/1‑251 (MQ=255)
                                          aaTCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGc                                                                                                                                                                                         <  1:102899/251‑1 (MQ=255)
                                          aaTCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGc                                                                                                                                                                                         <  1:817938/251‑1 (MQ=255)
                                            tCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCaa                                                                                                                                                                                       >  1:349458/1‑251 (MQ=255)
                                                aaCATCGTACCAACCTTAGTACATAGTCTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAAcgc                                                                                                                                                                                   <  1:20686/251‑1 (MQ=255)
                                                  cATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAAcgc                                                                                                                                                                                   >  1:36425/1‑249 (MQ=255)
                                                     cGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAt                                                                                                                                                                              <  1:473077/251‑1 (MQ=255)
                                                      gTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAtt                                                                                                                                                                             >  1:146436/1‑251 (MQ=255)
                                                       tACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATtg                                                                                                                                                                            <  1:485169/251‑1 (MQ=255)
                                                           aaCCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  <  1:663162/193‑1 (MQ=255)
                                                            aCCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGa                                                                                                                                                                       <  1:861586/251‑1 (MQ=255)
                                                             ccTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACatat                                                                                                                                                                                                      >  1:490227/1‑219 (MQ=255)
                                                               ttAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTAc                                                                                                                                                                                                          <  1:488730/213‑1 (MQ=255)
                                                                  gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:521922/1‑216 (MQ=255)
                                                                  gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:244989/1‑251 (MQ=255)
                                                                  gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:646649/1‑251 (MQ=255)
                                                                  gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:299868/1‑251 (MQ=255)
                                                                    aCATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGta                                                                                                                                                                                                                         >  1:242674/1‑193 (MQ=255)
                                                                       tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGTCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:152439/251‑1 (MQ=255)
                                                                       tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:806072/251‑1 (MQ=255)
                                                                       tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:545726/251‑1 (MQ=255)
                                                                       tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:391983/251‑1 (MQ=255)
                                                                       tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGACGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:73574/251‑1 (MQ=255)
                                                                                      tgtgAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaaga                                                                                                                                                                                                               <  1:415974/185‑1 (MQ=255)
                                                                                      tgtgAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAt                                                                                                                                                                              <  1:256217/218‑1 (MQ=255)
                                                                                                              cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaag                                                                                                                                                                                                                <  1:307529/160‑1 (MQ=255)
                                                                                                              cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:75192/1‑172 (MQ=255)
                                                                                                              cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCgg                                                                                                                      >  1:5718/1‑250 (MQ=255)
                                                                                                                 ttGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGtt                                                                                                                  <  1:579963/251‑1 (MQ=255)
                                                                                                                       cTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGCCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGa                                                                                                            >  1:782035/1‑251 (MQ=255)
                                                                                                                            attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  >  1:463794/1‑128 (MQ=255)
                                                                                                                            attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTgg                                                                                                                                                                         >  1:95620/1‑185 (MQ=255)
                                                                                                                            attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            >  1:693846/1‑198 (MQ=255)
                                                                                                                               cTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaaga                                                                                                                                                                                                               <  1:861683/144‑1 (MQ=255)
                                                                                                                               cTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAACTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCg                                                                                                                                   >  1:722538/1‑220 (MQ=255)
                                                                                                                                    ttCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGaa                                                                                               <  1:216246/251‑1 (MQ=255)
                                                                                                                                     tCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGaaa                                                                                              <  1:362457/251‑1 (MQ=255)
                                                                                                                                           tCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:190086/183‑1 (MQ=255)
                                                                                                                                           tCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAgccg                                                                                        >  1:59907/1‑251 (MQ=255)
                                                                                                                                            cGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAgccg                                                                                        >  1:562417/1‑250 (MQ=255)
                                                                                                                                                     tattaGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTatat                                                                                                                                                                                                                      >  1:163033/1‑115 (MQ=255)
                                                                                                                                                     tattaGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCa                                                                              >  1:180814/1‑251 (MQ=255)
                                                                                                                                                              aaaaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:543371/164‑1 (MQ=255)
                                                                                                                                                                aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:475173/251‑1 (MQ=255)
                                                                                                                                                                aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:138932/251‑1 (MQ=255)
                                                                                                                                                                aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:616626/251‑1 (MQ=255)
                                                                                                                                                                         cAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcac                                                          <  1:532958/251‑1 (MQ=255)
                                                                                                                                                                             cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:547642/1‑250 (MQ=255)
                                                                                                                                                                             cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:869052/1‑250 (MQ=255)
                                                                                                                                                                             cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:507883/1‑250 (MQ=255)
                                                                                                                                                                             cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCacacac                                                      <  1:184539/251‑1 (MQ=255)
                                                                                                                                                                             cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCacacac                                                      <  1:264829/251‑1 (MQ=255)
                                                                                                                                                                                          aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  >  1:484991/1‑66 (MQ=255)
                                                                                                                                                                                          aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:526695/225‑1 (MQ=255)
                                                                                                                                                                                          aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   >  1:65141/1‑225 (MQ=255)
                                                                                                                                                                                           cATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATg                                        >  1:171309/1‑251 (MQ=255)
                                                                                                                                                                                           cATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATg                                        <  1:427494/251‑1 (MQ=255)
                                                                                                                                                                                             ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATc                                                                                                        >  1:62254/1‑185 (MQ=255)
                                                                                                                                                                                             ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:147221/1‑251 (MQ=255)
                                                                                                                                                                                             ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCAGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:438585/1‑251 (MQ=255)
                                                                                                                                                                                             ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACACCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:658522/1‑251 (MQ=255)
                                                                                                                                                                                                          cGAATTATTGGTCAATGTGACACCCAACGAAGCTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGATCGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGc                         <  1:378647/251‑1 (MQ=255)
                                                                                                                                                                                                            aaTTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCaa                       >  1:148364/1‑251 (MQ=255)
                                                                                                                                                                                                              ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTaa                                                                                                                                                                                                                   >  1:386450/1‑61 (MQ=255)
                                                                                                                                                                                                              ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATtgt                                                                                                                                                                           <  1:876742/101‑1 (MQ=255)
                                                                                                                                                                                                              ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGAcc                                                                                                                                               >  1:604258/1‑129 (MQ=255)
                                                                                                                                                                                                              ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGc                                                                               <  1:868106/193‑1 (MQ=255)
                                                                                                                                                                                                              ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAAtt                     >  1:316926/1‑251 (MQ=255)
                                                                                                                                                                                                                        aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAt                                                                                                                                                                  <  1:818772/100‑1 (MQ=255)
                                                                                                                                                                                                                        aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:281924/106‑1 (MQ=255)
                                                                                                                                                                                                                        aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCtt                                                                                                   >  1:647047/1‑163 (MQ=255)
                                                                                                                                                                                                                        aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:783639/195‑1 (MQ=255)
                                                                                                                                                                                                                        aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCCCACACTGAATGCGTGGATGAAAACGAACAAAAACAATTTGTGGTaaaa           >  1:723913/1‑251 (MQ=255)
                                                                                                                                                                                                                          gtgACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGa         >  1:648598/1‑251 (MQ=255)
                                                                                                                                                                                                                           tgACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGAc        <  1:710655/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCATACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTcc  >  1:712076/1‑250 (MQ=255)
                                                                                                                                                                                                                                  cAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTcc  >  1:225962/1‑250 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
ATGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTTGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTCC  >  1/862197‑862672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑