Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,722	A→G	intergenic (+41524/+11306)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,722	0	A	G	100.0%	196.4 / NA	68	intergenic (+41524/+11306)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/41);  total (27/41)

GGTTTGCGGCAATTGGTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCACCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGATAGGTGC  >  1/858478‑858962                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 ggTTTGCGGCAATTGGTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCA‑‑‑CATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCaa                                                                                                                                                                                                                                         >  1:644946/1‑251 (MQ=255)          cAATTGGTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAaaccaa                                                                                                                                                                                                                                    >  1:236070/1‑250 (MQ=255)             ttGGTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCg                                                                                                                                                                                                                                 >  1:826572/1‑250 (MQ=255)               ggTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCaa                                                                                                                                                                                                                              >  1:362913/1‑251 (MQ=255)                  tAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAacga                                                                                                                                                                                                                           <  1:633584/251‑1 (MQ=255)                        cGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGga                                                                                                                                                                                                                     >  1:845570/1‑251 (MQ=255)                             aTGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGgacgac                                                                                                                                                                                                                 >  1:419538/1‑250 (MQ=255)                                   aaGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAAc                                                                                                                                                                                                          <  1:48276/251‑1 (MQ=255)                                   aaGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAAc                                                                                                                                                                                                          <  1:816946/251‑1 (MQ=255)                                     ggTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACcg                                                                                                                                                                                                        >  1:200047/1‑251 (MQ=255)                                          tttctttcCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCg                                                                                                                                                                                                   >  1:462/1‑251 (MQ=255)                                             ctttcCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAAt                                                                                                                                                                                                >  1:204381/1‑251 (MQ=255)                                              tttcCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAAtt                                                                                                                                                                                               >  1:898461/1‑251 (MQ=255)                                                             ggTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACggg                                                                                                                                                                                >  1:182909/1‑251 (MQ=255)                                                                ttCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGa                                                                                                                                                                             >  1:414575/1‑251 (MQ=255)                                                                 tCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGAt                                                                                                                                                                            >  1:366622/1‑251 (MQ=255)                                                                    tAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTc                                                                                                                                                                         <  1:252369/251‑1 (MQ=255)                                                                         cGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCAc                                                                                                                                                                    <  1:461752/251‑1 (MQ=255)                                                                           gTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAacga                                                                                                                                                                                                                           >  1:82815/1‑194 (MQ=255)                                                                           gTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCAc                                                                                                                                                                    >  1:603771/1‑249 (MQ=255)                                                                           gTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCAc                                                                                                                                                                    >  1:177296/1‑249 (MQ=255)                                                                                aCGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTtc                                                                                                                                                             <  1:542607/251‑1 (MQ=255)                                                                                    cTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCGCCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTtcatca                                                                                                                                                         <  1:676215/251‑1 (MQ=255)                                                                                     ttCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCaa                                                                                                                                                        >  1:725357/1‑251 (MQ=255)                                                                                            tCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTc                                                                                                                                                 <  1:628136/251‑1 (MQ=255)                                                                                             cTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAATCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTc                                                                                                                                                 <  1:448794/250‑1 (MQ=255)                                                                                                 cgcgGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAg                                                                                                                                            <  1:493823/251‑1 (MQ=255)                                                                                                      cgAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAAc                                                                                                                                       <  1:814763/251‑1 (MQ=255)                                                                                                      cgAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAAc                                                                                                                                       <  1:149813/251‑1 (MQ=255)                                                                                                             ggCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGGAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAAc                                                                                                                                       <  1:176559/244‑1 (MQ=255)                                                                                                             ggCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACgg                                                                                                                                     <  1:142396/246‑1 (MQ=255)                                                                                                              gCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGAccgc                                                                                                                                <  1:872289/250‑1 (MQ=255)                                                                                                                     ccACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCAcg                                                                                                                        >  1:237259/1‑251 (MQ=255)                                                                                                                             tCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCa                                                                                                                >  1:472507/1‑251 (MQ=255)                                                                                                                                          ttttGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTtgatg                                                                                                   <  1:574781/251‑1 (MQ=255)                                                                                                                                           tttGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTtgatga                                                                                                  >  1:576103/1‑251 (MQ=255)                                                                                                                                                          gATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACAACGCGGACGACCGGTAACCGCGCCGAATTCGTTAc                                                                                                                                                                                         <  1:834325/149‑1 (MQ=255)                                                                                                                                                            ttttCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATcc                                                                                 <  1:9571/251‑1 (MQ=255)                                                                                                                                                            ttttCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATcc                                                                                 <  1:499722/251‑1 (MQ=255)                                                                                                                                                            ttttCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATcc                                                                                 <  1:240061/251‑1 (MQ=255)                                                                                                                                                                 aCTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGAtt                                                                            <  1:649898/251‑1 (MQ=255)                                                                                                                                                                    gcATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTgcg                                                                         >  1:481487/2‑251 (MQ=255)                                                                                                                                                                             ccGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAacac                                                                                        <  1:557435/227‑1 (MQ=255)                                                                                                                                                                               gTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTtgatg                                                                                                   >  1:408990/1‑214 (MQ=255)                                                                                                                                                                                tCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAAc                                                              <  1:30021/250‑1 (MQ=255)                                                                                                                                                                                tCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAAc                                                              <  1:368205/250‑1 (MQ=255)                                                                                                                                                                                     cACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGc                                                        <  1:396834/251‑1 (MQ=255)                                                                                                                                                                                       cGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGc                                                        >  1:877787/1‑249 (MQ=255)                                                                                                                                                                                          cTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATcc                                                                                 <  1:100738/221‑1 (MQ=255)                                                                                                                                                                                            aaTTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCAcgcg                                                                                                                      >  1:631115/1‑182 (MQ=255)                                                                                                                                                                                            aaTTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATc                                                                                  <  1:194265/218‑1 (MQ=255)                                                                                                                                                                                                          gAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAacac                                                                                        >  1:194951/1‑198 (MQ=255)                                                                                                                                                                                                           aaaCCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCcggcgg                                   <  1:632078/250‑1 (MQ=255)                                                                                                                                                                                                                       gAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGc                       <  1:566447/250‑1 (MQ=255)                                                                                                                                                                                                                       gAGTTCAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGc                       <  1:780622/250‑1 (MQ=255)                                                                                                                                                                                                                            cAGTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGc                                                                                                          >  1:563535/1‑162 (MQ=255)                                                                                                                                                                                                                             atttGAATAGCGCGGCGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTAc                                                                                                                                                                                   <  1:272414/86‑1 (MQ=255)                                                                                                                                                                                                                              gTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCAcgcg                                                                                                                      <  1:864322/148‑1 (MQ=255)                                                                                                                                                                                                                              gTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGAt                                                                             <  1:296682/189‑1 (MQ=255)                                                                                                                                                                                                                              gTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAg                                                         <  1:223046/209‑1 (MQ=255)                                                                                                                                                                                                                              gTTGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACa               >  1:555712/1‑251 (MQ=255)                                                                                                                                                                                                                                tGAATAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATa             <  1:231256/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaTAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATAc            <  1:356962/250‑1 (MQ=255)                                                                                                                                                                                                                                  aaTAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATAc            <  1:22861/250‑1 (MQ=255)                                                                                                                                                                                                                                    tAGCGCGACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGa         <  1:491567/251‑1 (MQ=255)                                                                                                                                                                                                                                       cgcgACGAATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGAt        <  1:41423/249‑1 (MQ=255)                                                                                                                                                                                                                                         cgacgaATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGATAGGt    <  1:650481/251‑1 (MQ=255)                                                                                                                                                                                                                                           acgaATTGCCGCCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGATAGGTGc  <  1:23968/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 GGTTTGCGGCAATTGGTTAACATCGGTGATGCCGAAGGTGTTTTCTTTCCAGCCCGGCAAGGTTTCGTAAATCGGTTCAACGCCTTCCCAATCTTTCGCGGCGAGCGGGGCGTATTCCACGATGTCACCGTTCGGCATTTTGTAGCCGACGCAGATTTTCACTTCATCGAAACCGTCCAGCACGTCTAATTTGGTCATGCAGAAACCGGAAATGGAGTTCAGTTGAATAGCGCGACGAATTGCCACCGCGTCAAACCAACCGCAACGACGCGGACGACCGGTAACCGCGCCGAATTCGTTACCTTTACGGGCGATTTCAGCACCTGTTTCATCAAATAATTCCGTAGTGAACGGACCGCCGCCCACGCGGGTGCAGTAGGCTTTGATGATTCCCAACACATAATCCAGATTGCGCGGACCGAAACCGGAGCCTGTCGCTACGCCGCCGGCGGTGGTGTTGGAGCTGGTTACATACGGATAGGTGC  >  1/858478‑858962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑