Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	835,900	A→G	intergenic (+18702/+34128)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	835,900	0	A	G	100.0%	188.3 / NA	64	intergenic (+18702/+34128)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/42);  total (22/42)

GTATTAAAGCGGCTTACGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATAATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGTCATACAAGGTTT  >  1/835657‑836144                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gTATTAAAGCGGCTTACGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACAGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGaa                                                                                                                                                                                                                                               >  1:427210/1‑251 (MQ=255)       aaGCGGCTTACGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGAc                                                                                                                                                                                                                                         <  1:364537/251‑1 (MQ=255)            gCTTACGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCa                                                                                                                                                                                                                                    >  1:627385/1‑251 (MQ=255)                 cGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAg                                                                                                                                                                                                                               <  1:885328/251‑1 (MQ=255)                 cGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAg                                                                                                                                                                                                                               <  1:70922/251‑1 (MQ=255)                   aCAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGcc                                                                                                                                                                                                                             <  1:132954/251‑1 (MQ=255)                   aCAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGcc                                                                                                                                                                                                                             <  1:761488/251‑1 (MQ=255)                               aaTTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAg                                                                                                                                                                                                                  <  1:768741/250‑1 (MQ=255)                               aaTTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAg                                                                                                                                                                                                                  <  1:266658/250‑1 (MQ=255)                               aaTTAGAAGGGCGTTTTGTGATTATGGTGGC‑AACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGt                                                                                                                                                                                                                 <  1:650557/250‑1 (MQ=255)                                aTTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGtt                                                                                                                                                                                                                <  1:874307/251‑1 (MQ=255)                                     aaGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAg                                                                                                                                                                                                            <  1:766671/250‑1 (MQ=255)                                                 tgATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAg                                                                                                                                                                                               <  1:710012/251‑1 (MQ=255)                                                 tgATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAg                                                                                                                                                                                               <  1:682611/251‑1 (MQ=255)                                                  gATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTcc                                                                                                                                                                                                                        <  1:556902/225‑1 (MQ=255)                                                   aTTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTg                                                                                                                                                                                             <  1:542973/251‑1 (MQ=255)                                                                aCCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCa                                                                                                                                                                                                                                    <  1:282453/199‑1 (MQ=255)                                                                aCCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAg                                                                                                                                                                                                                               <  1:340021/204‑1 (MQ=255)                                                                aCCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTcc                                                                                                                                                                                                                        <  1:669867/211‑1 (MQ=255)                                                                aCCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAg                                                                                                                                                                                                            <  1:108311/223‑1 (MQ=255)                                                                                      aTTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTActc                                                                                                                                                          <  1:829809/251‑1 (MQ=255)                                                                                          ggTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTc                                                                                                                                                       >  1:144591/1‑250 (MQ=255)                                                                                              tATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAAcc                                                                                                                                                  <  1:851553/251‑1 (MQ=255)                                                                                              tATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAAcc                                                                                                                                                  <  1:158985/251‑1 (MQ=255)                                                                                                      ggCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAAcc                                                                                                                                                  <  1:629656/243‑1 (MQ=255)                                                                                                        cATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCaa                                                                                                                                        <  1:179345/251‑1 (MQ=255)                                                                                                          tGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCaaaa                                                                                                                                      >  1:345550/1‑251 (MQ=255)                                                                                                                         gAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATg                                                                                                                        <  1:184672/250‑1 (MQ=255)                                                                                                                          aaCGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGa                                                                                                                      <  1:795002/251‑1 (MQ=255)                                                                                                                                     ccGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGAtt                                                                                                           <  1:561399/251‑1 (MQ=255)                                                                                                                                                  gttgtCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCaa                                                                                                                                        <  1:150896/209‑1 (MQ=255)                                                                                                                                                         gCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCaaaa                                                                                       >  1:863163/1‑251 (MQ=255)                                                                                                                                                                 aGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTc                                                                                                                                                       >  1:883694/1‑179 (MQ=255)                                                                                                                                                                       gACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTcaca                                                                         >  1:591157/1‑251 (MQ=255)                                                                                                                                                                           gcggGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAAt                                                                      >  1:197777/1‑250 (MQ=255)                                                                                                                                                                                    cAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCAc                                                                                                                                                <  1:140078/167‑1 (MQ=255)                                                                                                                                                                                    cAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTg                                                             >  1:881463/1‑250 (MQ=255)                                                                                                                                                                                    cAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTg                                                             >  1:887297/1‑250 (MQ=255)                                                                                                                                                                                    cAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTg                                                             >  1:583024/1‑250 (MQ=255)                                                                                                                                                                                      ggTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTc                                                                                                                                                       >  1:749099/1‑158 (MQ=255)                                                                                                                                                                                      ggTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAAcc                                                                                                                                                  <  1:155822/163‑1 (MQ=255)                                                                                                                                                                                       gTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGc                                                            <  1:400124/248‑1 (MQ=255)                                                                                                                                                                                          aaGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATc                                                       <  1:445368/250‑1 (MQ=255)                                                                                                                                                                                                  aataatGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTc                                                                                                                                                                                                          <  1:756192/95‑1 (MQ=255)                                                                                                                                                                                                               ttATCGGACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGa                                 <  1:682320/251‑1 (MQ=255)                                                                                                                                                                                                                    ggACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCaa                            >  1:168487/1‑251 (MQ=255)                                                                                                                                                                                                                     gACAGACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAg                           >  1:727121/1‑251 (MQ=255)                                                                                                                                                                                                                         gACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGa                                 <  1:32988/241‑1 (MQ=255)                                                                                                                                                                                                                         gACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCg                       >  1:306682/1‑251 (MQ=255)                                                                                                                                                                                                                         gACTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCg                       >  1:520751/1‑251 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTtata                                                                                                                                                                                                                   >  1:272025/1‑61 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAg                                                                                                                                                                                                            <  1:759327/68‑1 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGAtt                                                                                                           <  1:172051/165‑1 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATc                                                       <  1:568704/217‑1 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTaa                                                  >  1:832438/1‑222 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCggg                     >  1:870385/1‑251 (MQ=255)                                                                                                                                                                                                                           cTTTATGTCTGTCCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCggg                     >  1:131807/1‑251 (MQ=255)                                                                                                                                                                                                                                  tctgtcCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTg               <  1:510432/250‑1 (MQ=255)                                                                                                                                                                                                                                  tctgtcCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGt              <  1:492757/251‑1 (MQ=255)                                                                                                                                                                                                                                     gtcCGATAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGTCAt           >  1:751967/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTAACCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGTCATACaa       <  1:394474/250‑1 (MQ=255)                                                                                                                                                                                                                                             aCCCGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGa                                 <  1:184864/221‑1 (MQ=255)                                                                                                                                                                                                                                              cccGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGtt                   <  1:199294/234‑1 (MQ=255)                                                                                                                                                                                                                                              cccGATGATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGTCATACAAGGttt  >  1:342488/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GTATTAAAGCGGCTTACGACAAACCGGAAGAATTAGAAGGGCGTTTTGTGATTATGGTGGCAAACCTGGCACCGCGCAAAATGAAATTTGGTGTATCCGAAGGCATGATTTTAAGTGCGGGAACGGGCGGTGCCGATTTATTCTTGTTGTCTGCCGACGAAGGCGTGACGGCGGGAATGCAGGTGAAGTAGTCAATAATGACAAATTTATCGGACAGACTTTATGTCTGTCCGATAACCCGATAATATGAAAAGGACAGGCATAAAGCCTGTCCTTATAGTTAAAGTCTGCGATTAAAGTGCGGTTGCTTTTCCGAGAGAATTTTTACTCGTACTCTTCCAACCACTTCAGCAAAATGGCTTCTAAAATGGATTCGTTAGATTTTTGCGGATCGTCATCAAAATTGTCCAGCTCACAAATCCAGCGGTGCAGATCGGTAAAACGCACGGTTTTTGGATCCAAGTCCGGGTTATTGTCATACAAGGTTT  >  1/835657‑836144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑