Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	795,324	C→T	intergenic (+8048/‑21788)	unknown → / → unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	795,324	0	C	T	100.0%	201.5 / NA	71	intergenic (+8048/‑21788)	unknown/unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/41);  total (30/41)

GCTAATTAAAATTGCTCCCCAAATACGTTTTAAGGTTATGGAGAAGCTGTGTTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGCAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGGGATAAAAACATAAAAAACTCCATTTTGATTTAACACAATGTTTTCCTCACGCAGGATGATGT  >  1/795081‑795559                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            gCTAATTAAAATTGCTCCCCAAATACGTTTTAAGGTTATGGAGAAGCTGTGTTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATg                                                                                                                                                                                                                                      >  1:841701/1‑251 (MQ=255)                             tttAAGGTTATGGAGAAGCTGTGTTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATagag                                                                                                                                                                                                          <  1:355565/251‑1 (MQ=255)                                            aaGCTGTGTTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAAt                                                                                                                                                                                           <  1:731396/251‑1 (MQ=255)                                                 gtgtTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATagag                                                                                                                                                                                                          <  1:168579/231‑1 (MQ=255)                                                 gtgtTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATCTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGaca                                                                                                                                                                                       >  1:188365/1‑250 (MQ=255)                                                           gTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATgg                                                                                                                                                                                                                                >  1:565669/1‑199 (MQ=255)                                                           gTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAatat                                                                                                                                                                                              >  1:820043/1‑233 (MQ=255)                                                             aTTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCAt                                                                                                                                                                          <  1:49044/251‑1 (MQ=255)                                                                   gTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAAt                                                                                                                                                                                           <  1:620912/228‑1 (MQ=255)                                                                              tGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAg                                                                                                                                                         <  1:607423/251‑1 (MQ=255)                                                                                    ggTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGacac                                                                                                                                                                                      >  1:115930/1‑216 (MQ=255)                                                                                         aTACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAAt                                                                                                                                              >  1:785819/1‑251 (MQ=255)                                                                                         aTACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAAt                                                                                                                                              <  1:756012/251‑1 (MQ=255)                                                                                         aTACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAAt                                                                                                                                              >  1:640698/1‑251 (MQ=255)                                                                                           aCTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGa                                                                                                                                            <  1:401419/251‑1 (MQ=255)                                                                                           aCTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGa                                                                                                                                            <  1:27836/251‑1 (MQ=255)                                                                                              cACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAg                                                                                                                                                                                                                  >  1:877284/1‑178 (MQ=255)                                                                                               aCCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTg                                                                                                                                        >  1:321586/1‑251 (MQ=255)                                                                                               aCCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTg                                                                                                                                        >  1:659071/1‑251 (MQ=255)                                                                                                  aaGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGt                                                                                                                                     <  1:695973/251‑1 (MQ=255)                                                                                                    gCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTaa                                                                                                                                   >  1:852341/1‑251 (MQ=255)                                                                                                      aCCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATgg                                                                                                                                                                                                                                <  1:256384/156‑1 (MQ=255)                                                                                                               tGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGc                                                                                                                        <  1:209424/251‑1 (MQ=255)                                                                                                                gATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGt                                                                                                                                <  1:448882/242‑1 (MQ=255)                                                                                                                            gATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACt                                                                                                            >  1:827484/1‑250 (MQ=255)                                                                                                                                          gAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGttt                                                                                                                                                                               <  1:521346/169‑1 (MQ=255)                                                                                                                                               aagaagATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAg                                                                                        <  1:25983/251‑1 (MQ=255)                                                                                                                                                      ttACTAAATCTATAGCTTATCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTaa                                                                                 >  1:674138/1‑251 (MQ=255)                                                                                                                                                            aaTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:64430/251‑1 (MQ=255)                                                                                                                                                            aaTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:33610/251‑1 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATgg                                                                                                                                                                                                                                <  1:190450/101‑1 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCg                                                                                                                                                                                                                              >  1:727522/1‑103 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACa                                                                                                                                                                                                  <  1:623133/131‑1 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAAt                                                                                                                                                                                           >  1:572248/1‑138 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGacac                                                                                                                                                                                      >  1:421884/1‑143 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAAt                                                                                                                                                              <  1:85817/167‑1 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTc                                                                                                           <  1:560461/218‑1 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAAtt                                                                               >  1:574795/1‑246 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATtata                                                                            >  1:389145/1‑249 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATtata                                                                            >  1:558156/1‑249 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           >  1:889881/1‑250 (MQ=255)                                                                                                                                                             aTCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:654650/250‑1 (MQ=255)                                                                                                                                                                 ataGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTcc                                                                      <  1:387993/251‑1 (MQ=255)                                                                                                                                                                   aGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGc                                                                                                                                                        >  1:324844/1‑167 (MQ=255)                                                                                                                                                                      ttGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAAttt                                                                                                      >  1:47525/1‑214 (MQ=255)                                                                                                                                                                        gTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGa                                                                                                                                                                                         <  1:772919/129‑1 (MQ=255)                                                                                                                                                                           ccTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATag                                                                                                                                                                                                            <  1:84323/107‑1 (MQ=255)                                                                                                                                                                            ctctGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGacac                                                                                                                                                                                      <  1:297909/128‑1 (MQ=255)                                                                                                                                                                              ctGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGa                                                                                                                                                                                         <  1:788594/123‑1 (MQ=255)                                                                                                                                                                                gACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:811845/231‑1 (MQ=255)                                                                                                                                                                                       cAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAAt                                                                                >  1:490158/1‑219 (MQ=255)                                                                                                                                                                                         ggAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGc                                                                                                                                                                                                                                     <  1:856835/68‑1 (MQ=255)                                                                                                                                                                                         ggAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGAt                                                                                                                                           <  1:303111/158‑1 (MQ=255)                                                                                                                                                                                           agagaTTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACt                                             >  1:417093/1‑250 (MQ=255)                                                                                                                                                                                                tttGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCAttt                                       <  1:459793/251‑1 (MQ=255)                                                                                                                                                                                                 ttGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCAtttt                                      >  1:757393/1‑251 (MQ=255)                                                                                                                                                                                                     ttttCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCATTTTGAtt                                  <  1:187544/251‑1 (MQ=255)                                                                                                                                                                                                            ttAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCATTTTGATTTAacac                            <  1:560458/250‑1 (MQ=255)                                                                                                                                                                                                                ggAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAAttt                                                                                                      >  1:222385/1‑172 (MQ=255)                                                                                                                                                                                                                 gAAATAATGACTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCAt                                                                                                                 <  1:681086/160‑1 (MQ=255)                                                                                                                                                                                                                           cTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCttt                                                                                                                                                 >  1:131779/1‑118 (MQ=255)                                                                                                                                                                                                                           cTTCATTTGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           >  1:537470/1‑188 (MQ=255)                                                                                                                                                                                                                                tttGGCAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCATTTTGATTTAACACAATGTTTTCCTCACGCAGgat       <  1:559307/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCATTTTGATTTAACACAATGTTTTCCTCACGCAGGATGATGt  <  1:130349/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAACATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGAGATAAAAACATAAAAAACTCCATTTTGATTTAACACAATGTTTTCCTCACGCAGGATGATGt  <  1:246653/251‑1 (MQ=255)                                                                                                                                                                                                                                       aCATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:337683/176‑1 (MQ=255)                                                                                                                                                                                                                                       aCATTGACGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTg                                                                         <  1:380949/178‑1 (MQ=255)                                                                                                                                                                                                                                              cGATTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCAACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATAc                                                                           <  1:530314/169‑1 (MQ=255)                                                                                                                                                                                                                                                aTTGTAACAATGCTATGGCGATGTTGGCAAAGAAATagag                                                                                                                                                                                                          >  1:319184/1‑40 (MQ=255)                                                                                                                                                                                                                                                aTTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAAttt                                                                                                      >  1:396727/1‑140 (MQ=255)                                                                                                                                                                                                                                                aTTGTAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTgaga                                                              <  1:272110/180‑4 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            GCTAATTAAAATTGCTCCCCAAATACGTTTTAAGGTTATGGAGAAGCTGTGTTGGAAGGTATTAAAGTGACGCAGACTGATTTGGTGAATACTCACCAAGCACCATGCACTGATAAAAATATAGATAAATTGTTTCAGAAAAAAGAAGATTACTAAATCTATAGCTTGTCCCTCTGACATCGCAGGAGAGATTTGTTTTTCGGTTAAGGAAATAATGACTTCATTTGGCAACATTGACGATTGCAACAATGCTATGGCGATGTTGGCAAAGAAATAGAGTGCCGACAATATAATGACACTTTGTTTTTCATTTTGTATGAAATGGAAGCTATCTTTAATGATTTGGGTAAAGTTAATTGGCATTTCATCTACTCAATTTGATGTGAAATAAAGTGCTTAATTATACTGTCCTTTTGGGATAAAAACATAAAAAACTCCATTTTGATTTAACACAATGTTTTCCTCACGCAGGATGATGT  >  1/795081‑795559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑