Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	793,277	T→G	intergenic (+6001/‑23835)	unknown → / → unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	793,277	0	T	G	100.0%	144.5 / NA	49	intergenic (+6001/‑23835)	unknown/unknown	tRNA‑Leu‑GAG/tRNA‑Leu‑CAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/29);  total (20/29)

TTACGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATTTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAATTGTGCGATGACGGTGGAGCCTGAAAAACCCGCGTCGCCCGGGTTTTCATC  >  1/793035‑793510                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          ttACGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                                                                                                   <  1:599295/251‑1 (MQ=255)       gggACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCtg                                                                                                                                                                                                                              <  1:808297/250‑1 (MQ=255)                              aGCGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGgaga                                                                                                                                                                                                                   >  1:172022/1‑238 (MQ=255)                                cGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTg                                                                                                                                                                                                      <  1:363503/249‑1 (MQ=255)                                cGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGt                                                                                                                                                                                                     <  1:538376/250‑1 (MQ=255)                                  ttGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTaa                                                                                                                                                                                                  <  1:727687/251‑1 (MQ=255)                                    ggCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAAt                                                                                                                                                                                                >  1:791322/1‑251 (MQ=255)                                                 aCCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAg                                                                                                                                                                                   >  1:67230/1‑251 (MQ=255)                                                    ccAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                                                                                                   >  1:631957/1‑200 (MQ=255)                                                    ccAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                                                                                                   <  1:811818/200‑1 (MQ=255)                                                          gggTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAgcgc                                                                                                                                                                                >  1:512527/1‑245 (MQ=255)                                                          gggTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTAtct                                                                                                                                                                           >  1:379704/1‑250 (MQ=255)                                                            gTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTa                                                                                                                                                                                     <  1:667861/238‑1 (MQ=255)                                                               aGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAAc                                                                                                                                                                      <  1:398261/250‑1 (MQ=255)                                                                        gtTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                                                                                                   <  1:100100/180‑1 (MQ=255)                                                                             cGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAac                                                                                                                                                       <  1:839122/251‑1 (MQ=255)                                                                                     cGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATACTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCtt                                                                                                                                               >  1:839088/1‑251 (MQ=255)                                                                                      gAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAac                                                                                                                                                       <  1:628045/242‑1 (MQ=255)                                                                                       aaTAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAgc                                                                                                                                                                                  >  1:129069/1‑214 (MQ=255)                                                                                              tGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTaa                                                                                                                                                                                                  <  1:413968/191‑1 (MQ=255)                                                                                                    aaGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACaa                                                                                                                                 <  1:169253/250‑1 (MQ=255)                                                                                                                  ggATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAAc                                                                                                                                   <  1:239702/234‑1 (MQ=255)                                                                                                                      ttATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGcc                                                                                                              <  1:311880/251‑1 (MQ=255)                                                                                                                      ttATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGcc                                                                                                              <  1:69794/251‑1 (MQ=255)                                                                                                                                gTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAAc                                                                                                                                           <  1:711034/212‑1 (MQ=255)                                                                                                                                  ttCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACaa                                                                                                                                 >  1:470071/1‑220 (MQ=255)                                                                                                                                  ttCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATAc                                                                                                                 <  1:453825/236‑1 (MQ=255)                                                                                                                                    ccACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAgc                                                                                                                                                                                  <  1:774019/169‑1 (MQ=255)                                                                                                                                      acaAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGa                                                                                              <  1:177224/251‑1 (MQ=255)                                                                                                                                         aGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                                                                                                   >  1:522047/1‑115 (MQ=255)                                                                                                                                         aGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTGTTGATTAAAACGCTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGa                                                                                              <  1:868979/248‑1 (MQ=255)                                                                                                                                          gATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACt                                                                                                                            >  1:844402/1‑217 (MQ=255)                                                                                                                                                             gaTTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTg                                                                        >  1:827490/1‑250 (MQ=255)                                                                                                                                                                       ccTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACaa                                                                                                                                 >  1:604560/1‑183 (MQ=255)                                                                                                                                                                       ccTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACaa                                                                                                                                 >  1:862035/1‑183 (MQ=255)                                                                                                                                                                       ccTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAgg                                                             >  1:273199/1‑251 (MQ=255)                                                                                                                                                                       ccTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAgg                                                             <  1:180778/251‑1 (MQ=255)                                                                                                                                                                                ttCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAAt                                                    <  1:548855/251‑1 (MQ=255)                                                                                                                                                                                ttCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAAt                                                    <  1:304999/251‑1 (MQ=255)                                                                                                                                                                                      cGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATAc                                                                                                                 >  1:855844/1‑184 (MQ=255)                                                                                                                                                                                          tCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAATTGTGCGATGa                                          <  1:401699/251‑1 (MQ=255)                                                                                                                                                                                              cTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGc                                                            <  1:722167/229‑1 (MQ=255)                                                                                                                                                                                                              gCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAAt                                                    >  1:786204/1‑221 (MQ=255)                                                                                                                                                                                                               ccAAGTTTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAATTGTGCGATGACGGTGGAGCCTGAAAAAccc                      >  1:653219/1‑250 (MQ=255)                                                                                                                                                                                                                                  atataAATAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAATTGTGCGATGACGGTGGAGCCTGAAAAACCCGCGTCGCCCGGGTTTtcatc  <  1:588997/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaTAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCaa                                                      <  1:344580/194‑1 (MQ=255)                                                                                                                                                                                                                                         tAGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGc                                                                                                                                                                                              <  1:661859/56‑1 (MQ=255)                                                                                                                                                                                                                                          aGTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGc                                                                                                                                                                                              >  1:397462/1‑55 (MQ=255)                                                                                                                                                                                                                                           gTTACAATGTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTAtct                                                                                                                                                                           >  1:172484/1‑73 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          TTACGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGACCCCAAAGGGGTGAGCAAGTAAGTTTTCGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTCCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCTCAGGGCTTCGCCCGGCTCCCCTCGTGAACGAGGGAAGCCAAGTTTTTTTTCTTTTTATATAAATAGTTACAATTTTTCGAACAGCTGTGGTCGGGAGACGCGGGCTATCTGTTAAATGCCATCTATTAAGCGCTATCTCAAACGCTATTGATTAAAACACTTTCTTAAACGGTTTAACAATCACTTCGCCATATACGCCGGCTTCTACATAAGGATCCTGTGCTGCCCAGTCTTTTGCGGCTTGCAGGCTATCAAATTGTGCGATGACGGTGGAGCCTGAAAAACCCGCGTCGCCCGGGTTTTCATC  >  1/793035‑793510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑